1887

Abstract

Dragonfly cyclovirus (DfCyV), a new species of ssDNA virus discovered using viral metagenomics in dragonflies (familyLibellulidae) from the Kingdom of Tonga. Metagenomic sequences of DfCyV were similar to viruses of the recently proposed genus Cyclovirus within the family. Specific PCRs resulted in the recovery of 21 DfCyV genomes from three dragonfly species (, and ). The 1741 nt DfCyV genomes share >95 % nucleotide identity and are classified into 11 subtypes representing a single strain. The DfCyV genomes share 48–63 % genome-wide nucleotide identity with cycloviruses identified in human faecal samples. Recombination analysis revealed three recombinant DfCyV genomes, suggesting that recombination plays an important role in cyclovirus evolution. To our knowledge, this is the first report of a circular ssDNA virus identified in insects, and the data may help elucidate evolutionary links among novel recently identified in animals and environmental samples.

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2011-06-01
2019-09-20
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References

  1. Altschul S. F. , Madden T. L. , Schäffer A. A. , Zhang J. H. , Zhang Z. , Miller W. , Lipman D. J. . ( 1997; ). Gapped blast and psi-blast: a new generation of protein database search programs. . Nucleic Acids Res 25:, 3389–3402. [CrossRef] [PubMed]
    [Google Scholar]
  2. Anderson R. C. . ( 2009; ). Do dragonflies migrate across the western Indian Ocean?. J Trop Ecol 25:, 347–358. [CrossRef]
    [Google Scholar]
  3. Blinkova O. , Victoria J. , Li Y. , Keele B. F. , Sanz C. , Ndjango J.-B. N. , Peeters M. , Travis D. , Lonsdorf E. V. et al. ( 2010; ). Novel circular DNA viruses in stool samples of wild-living chimpanzees. . J Gen Virol 91:, 74–86. [CrossRef] [PubMed]
    [Google Scholar]
  4. Carstens E. B. . ( 2010; ). Ratification vote on taxonomic proposals to the International Committee on Taxonomy of Viruses (2009). . Arch Virol 155:, 133–146. [CrossRef] [PubMed]
    [Google Scholar]
  5. Charpentier R. . ( 1979; ). A nonoccluded virus in nymphs of the dragonfly Leucorrhinia dubia (Odonata, Anisoptera). . J Invertebr Pathol 34:, 95–98. [CrossRef]
    [Google Scholar]
  6. Corbet P. S. . ( 1999; ). Dragonflies: Behaviour and Ecology of Odonata. Colchester, UK:: Harley Books;.
    [Google Scholar]
  7. Corbet P. , Brooks S. . ( 2008; ). Dragonflies: In New Naturalist Library, London:: Harper-Collins;.
    [Google Scholar]
  8. Desbiez C. , David C. , Mettouchi A. , Laufs J. , Gronenborn B. . ( 1995; ). Rep protein of tomato yellow leaf curl geminivirus has an ATPase activity required for viral DNA replication. . Proc Natl Acad Sci U S A 92:, 5640–5644. [CrossRef] [PubMed]
    [Google Scholar]
  9. Duffy S. , Shackelton L. A. , Holmes E. C. . ( 2008; ). Rates of evolutionary change in viruses: patterns and determinants. . Nat Rev Genet 9:, 267–276. [CrossRef] [PubMed]
    [Google Scholar]
  10. Fauquet C. M. , Mayo M. A. , Maniloff J. , Desselberger U. , Ball L. A. . ( 2005; ). Virus Taxonomy: Eighth Report of the International Committee on Taxonomy of Viruses. San Diego, CA:: Academic Press;.
    [Google Scholar]
  11. Guindon S. P. , Gascuel O. . ( 2003; ). A simple, fast, and accurate algorithm to estimate large phylogenies by maximum likelihood. . Syst Biol 52:, 696–704. [CrossRef] [PubMed]
    [Google Scholar]
  12. Heath L. , Williamson A. L. , Rybicki E. P. . ( 2006; ). The capsid protein of beak and feather disease virus binds to the viral DNA and is responsible for transporting the replication-associated protein into the nucleus. . J Virol 80:, 7219–7225. [CrossRef] [PubMed]
    [Google Scholar]
  13. Hogenhout S. A. , Ammar D. , Whitfield A. E. , Redinbaugh M. G. . ( 2008; ). Insect vector interactions with persistently transmitted viruses. . Annu Rev Phytopathol 46:, 327–359. [CrossRef] [PubMed]
    [Google Scholar]
  14. Ilyina T. V. , Koonin E. V. . ( 1992; ). Conserved sequence motifs in the initiator proteins for rolling circle DNA replication encoded by diverse replicons from eubacteria, eucaryotes and archaebacteria. . Nucleic Acids Res 20:, 3279–3285. [CrossRef] [PubMed]
    [Google Scholar]
  15. Kapoor A. , Simmonds P. , Lipkin W. I. , Zaidi S. , Delwart E. . ( 2010; ). Use of nucleotide composition analysis to infer hosts for three novel picorna-like viruses. . J Virol 84:, 10322–10328. [CrossRef] [PubMed]
    [Google Scholar]
  16. Kim K. H. , Chang H. W. , Nam Y. D. , Roh S. W. , Kim M. S. , Sung Y. , Jeon C. O. , Oh H. M. , Bae J. W. . ( 2008; ). Amplification of uncultured single-stranded DNA viruses from rice paddy soil. . Appl Environ Microbiol 74:, 5975–5985. [CrossRef] [PubMed]
    [Google Scholar]
  17. Lefeuvre P. , Martin D. P. , Hoareau M. , Naze F. , Delatte H. , Thierry M. , Varsani A. , Becker N. , Reynaud B. , Lett J. M. . ( 2007; ). Begomovirus ‘melting pot’ in the south-west Indian Ocean islands: molecular diversity and evolution through recombination. . J Gen Virol 88:, 3458–3468. [CrossRef] [PubMed]
    [Google Scholar]
  18. Lefeuvre P. , Lett J. M. , Varsani A. , Martin D. P. . ( 2009; ). Widely conserved recombination patterns among single-stranded DNA viruses. . J Virol 83:, 2697–2707. [CrossRef] [PubMed]
    [Google Scholar]
  19. Li L. , Kapoor A. , Slikas B. , Bamidele O. S. , Wang C. , Shaukat S. , Masroor M. A. , Wilson M. L. , Ndjango J.-B. N. et al. ( 2010; a). Multiple diverse circoviruses infect farm animals and are commonly found in human and chimpanzee feces. . J Virol 84:, 1674–1682. [CrossRef] [PubMed]
    [Google Scholar]
  20. Li L. , Victoria J. G. , Wang C. , Jones M. , Fellers G. M. , Kunz T. H. , Delwart E. . ( 2010; b). Bat guano virome: predominance of dietary viruses from insects and plants plus novel mammalian viruses. . J Virol 84:, 6955–6965. [CrossRef] [PubMed]
    [Google Scholar]
  21. Liu Q. G. , Tikoo S. K. , Babiuk L. A. . ( 2001; ). Nuclear localization of the ORF2 protein encoded by porcine circovirus type 2. . Virology 285:, 91–99. [CrossRef] [PubMed]
    [Google Scholar]
  22. López-Bueno A. , Tamames J. , Velázquez D. , Moya A. , Quesada A. , Alcamí A. . ( 2009; ). High diversity of the viral community from an Antarctic lake. . Science 326:, 858–861. [CrossRef] [PubMed]
    [Google Scholar]
  23. Martin D. P. , Williamson C. , Posada D. . ( 2005; ). rdp2: recombination detection and analysis from sequence alignments. . Bioinformatics 21:, 260–262. [CrossRef] [PubMed]
    [Google Scholar]
  24. Meerts P. , Misinzo G. , McNeilly F. , Nauwynck H. J. . ( 2005; ). Replication kinetics of different porcine circovirus 2 strains in PK-15 cells, fetal cardiomyocytes and macrophages. . Arch Virol 150:, 427–441. [CrossRef] [PubMed]
    [Google Scholar]
  25. Rosario K. , Duffy S. , Breitbart M. . ( 2009; ). Diverse circovirus-like genome architectures revealed by environmental metagenomics. . J Gen Virol 90:, 2418–2424. [CrossRef] [PubMed]
    [Google Scholar]
  26. Tamura K. , Dudley J. , Nei M. , Kumar S. . ( 2007; ). mega4: Molecular evolutionary genetics analysis (mega) software version 4.0. . Mol Biol Evol 24:, 1596–1599. [CrossRef] [PubMed]
    [Google Scholar]
  27. Theischinger G. , Hawking J. . ( 2006; ). The Complete Field Guide to Dragonflies of Australia. Collingwood:: CSIRO;.
    [Google Scholar]
  28. Thompson J. D. , Higgins D. G. , Gibson T. J. . ( 1994; ). clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. . Nucleic Acids Res 22:, 4673–4680. [CrossRef] [PubMed]
    [Google Scholar]
  29. Todd D. . ( 2000; ). Circoviruses: immunosuppressive threats to avian species: a review. . Avian Pathol 29:, 373–394. [CrossRef] [PubMed]
    [Google Scholar]
  30. Varsani A. , Shepherd D. N. , Monjane A. L. , Owor B. E. , Erdmann J. B. , Rybicki E. P. , Peterschmitt M. , Briddon R. W. , Markham P. G. et al. ( 2008; ). Recombination, decreased host specificity and increased mobility may have driven the emergence of maize streak virus as an agricultural pathogen. . J Gen Virol 89:, 2063–2074. [CrossRef] [PubMed]
    [Google Scholar]
  31. Varsani A. , Regnard G. L. , Bragg R. R. , Hitzeroth I. I. , Rybicki E. P. . ( 2011; ). Global genetic diversity and geographical and host species distribution of beak and feather disease virus isolates. . J Gen Virol 92:, 752–767.[PubMed] [CrossRef]
    [Google Scholar]
  32. Walker J. E. , Saraste M. , Runswick M. J. , Gay N. J. . ( 1982; ). Distantly related sequences in the α- and β-subunits of ATP synthase, myosin, kinases and other ATP-requiring enzymes and a common nucleotide binding fold. . EMBO J 1:, 945–951.[PubMed]
    [Google Scholar]
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vol. , part 6, pp. 1302–1308

Sequence annotation of DfCyV genomes. Two-dimensional graphical representation of pairwise nucleotide and amino acid sequence identities of Cap and Rep ORFs.

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