RT Journal Article SR Electronic(1) A1 Orba, Yasuko A1 Kobayashi, Shintaro A1 Nakamura, Ichiro A1 Ishii, Akihiro A1 Hang'ombe, Bernard M. A1 Mweene, Aaron S. A1 Thomas, Yuka A1 Kimura, Takashi A1 Sawa, HirofumiYR 2011 T1 Detection and characterization of a novel polyomavirus in wild rodents JF Journal of General Virology, VO 92 IS 4 SP 789 OP 795 DO https://doi.org/10.1099/vir.0.027854-0 PB Microbiology Society, SN 1465-2099, AB To investigate polyomavirus infection in wild rodents, we analysed DNA samples from the spleens of 100 wild rodents from Zambia using a broad-spectrum PCR-based assay. A previously unknown polyomavirus genome was identified in a sample from a multimammate mouse (Mastomys species) and the entire viral genome of 4899 bp was subsequently sequenced. This viral genome contained potential ORFs for the capsid proteins, VP1, VP2 and VP3, and early proteins, small t antigen and large T antigen. Phylogenetic analysis showed that it was a novel member of the family Polyomaviridae, and thus the virus was tentatively named mastomys polyomavirus. After transfection of the viral genome into several mammalian cell lines, transient expression of the VP1 and large T antigen proteins was confirmed by immunoblotting and immunocytochemical analyses. Comparison of large T antigen function in mastomys polyomavirus with that in rhesus monkey polyomavirus SV40 and human polyomavirus JC virus revealed that the large T antigen from mastomys polyomavirus interacted with the tumour suppressor protein pRb, but not with p53., UL https://www.microbiologyresearch.org/content/journal/jgv/10.1099/vir.0.027854-0