1887

Journal of General Virology header logo

Volume 92, Issue 4

Intrafamiliar transmission of Kaposi's sarcoma-associated herpesvirus and seronegative infection in family members of classic Kaposi's sarcoma patients Free

Abstract

The link between Kaposi's sarcoma-associated herpesvirus (KSHV or human herpesvirus 8) and Kaposi's sarcoma has been proven, but the transmission routes, especially in the heterosexual population, are not yet completely understood. To assess the intrafamilial patterns of transmission among first-degree relatives of Italian classic Kaposi's sarcoma (cKS) patients, KSHV seroprevalence and the presence of viral DNA in blood and saliva were evaluated in 18 families (32 cKS patients and 35 family members), comparing the results with those obtained in 200 elderly healthy controls without known exposure to KSHV. The KSHV genotype of variable region VR1 of the hypervariable ORF K1 gene was subsequently analysed in all KSHV-positive samples. The results showed that KSHV infection was significantly higher in relatives of cKS patients (11/35 cases) than in healthy controls (17/200 cases; =0.001). The 11 infected relatives included spouses (=3), siblings (=2) and offspring (=6) of the cKS patients; the same KSHV genotype was shared within the same family in the majority of cases (85 %), indicating the presence of person-to-person transmission within families. Viral DNA was mostly observed in the saliva of infected relatives (45.4 %); detection of DNA in blood was less frequent (27.3 %). Notably, KSHV DNA was present in saliva and/or blood of three KSHV-infected relatives with indeterminate or negative serostatus. Thus, the risk of KSHV infection is greatly enhanced within families of cKS patients, where close contacts (horizontal and/or sexual) can contribute to the spread of KSHV.

  • Received:
  • Accepted:
  • Published Online:
© SGM
Loading

Article metrics loading...

/content/journal/jgv/10.1099/vir.0.027847-0
2011-04-01
2024-04-24
Loading full text...

Full text loading...

/deliver/fulltext/jgv/92/4/744.html?itemId=/content/journal/jgv/10.1099/vir.0.027847-0&mimeType=html&fmt=ahah

References

  1. Angeloni, A., Heston, L., Uccini, S., Sirianni, M. C., Cottoni, F., Masala, M. V., Cerimele, D., Lin, S. F., Sun, R. & other authors(1998). High prevalence of antibodies to human herpesvirus 8 in relatives of patients with classic Kaposi's sarcoma from Sardinia. J Infect Dis 177, 1715–1718. [CrossRef] [Google Scholar]
  2. Atzori, L., Fadda, D., Ferreli, C., Pastorelli, C., Iannelli, P., Rais, M., Faa, G., Cocco, P. & Aste, N.(2004). Classic Kaposi's sarcoma in southern Sardinia, Italy. Br J Cancer 91, 1261–1262. [CrossRef] [Google Scholar]
  3. Blackbourn, D. J., Lennette, E. T., Ambroziak, J., Mourich, D. V. & Levy, J. A.(1998). Human herpesvirus 8 detection in nasal secretions and saliva. J Infect Dis 177, 213–216. [CrossRef] [Google Scholar]
  4. Bourboulia, D., Whitby, D., Boshoff, C., Newton, R., Beral, V., Carrara, H., Lane, A. & Sitas, F.(1998). Serologic evidence for mother-to-child transmission of Kaposi sarcoma-associated herpesvirus infection. JAMA 280, 31–32. [CrossRef] [Google Scholar]
  5. Brambilla, L., Boneschi, V., Ferrucci, S., Taglioni, M. & Berti, E.(2000). Human herpesvirus-8 infection among heterosexual partners of patients with classical Kaposi's sarcoma. Br J Dermatol 143, 1021–1025. [CrossRef] [Google Scholar]
  6. Calabrò, M. L., Sheldon, J., Favero, A., Simpson, G. R., Fiore, J. R., Gomes, E., Angarano, G., Chieco-Bianchi, L. & Schulz, T. F.(1998). Seroprevalence of Kaposi's sarcoma-associated herpesvirus/human herpesvirus 8 in several regions of Italy. J Hum Virol 1, 207–213. [Google Scholar]
  7. Cannon, M. J., Dollard, S. C., Smith, D. K., Klein, R. S., Schuman, P., Rich, J. D., Vlahov, D. & Pellett, P. E., HIV Epidemiology Research Study Group(2001). Blood-borne and sexual transmission of human herpesvirus 8 in women with or at risk for human immunodeficiency virus infection. N Engl J Med 344, 637–643. [CrossRef] [Google Scholar]
  8. Casper, C., Krantz, E., Selke, S., Kuntz, S. R., Wang, J., Huang, M. L., Pauk, J. S., Corey, L. & Wald, A.(2007). Frequent and asymptomatic oropharyngeal shedding of human herpesvirus 8 among immunocompetent men. J Infect Dis 195, 30–36. [CrossRef] [Google Scholar]
  9. Challine, D., Roudot-Thoraval, F., Sarah, T., Laperche, L., Boisson, B., Mauberquez, S., Dubernet, F., Rigot, P., Lefrère, F. & other authors(2001). Seroprevalence of human herpes virus 8 antibody in populations at high or low risk of transfusion, graft, or sexual transmission of viruses. Transfusion 41, 1120–1125. [CrossRef] [Google Scholar]
  10. Cook, R. D., Hodgson, T. A., Waugh, A. C., Molyneux, E. M., Borgstein, E., Sherry, A., Teo, C. G. & Porter, S. R.(2002). Mixed patterns of transmission of human herpesvirus-8 (Kaposi's sarcoma-associated herpesvirus) in Malawian families. J Gen Virol 83, 1613–1619. [Google Scholar]
  11. Crispo, A., Tamburini, M., De Marco, M. R., Ascierto, P., Silvestro, P., Ronga, D., Tridente, V., Desicato, S., Carbone, S. & other authors(2001). HHV-8 prevalence, immunosuppression and Kaposi's sarcoma in South Italy. Int J Mol Med 7, 535–538. [Google Scholar]
  12. Dal Maso, L., Polesel, J., Ascoli, V., Zambon, P., Budroni, M., Ferretti, S., Tumino, R., Tagliabue, G., Patriarca, S. & other authors(2005). Cancer and AIDS Registry Linkage Study. Classic Kaposi's sarcoma in Italy, 1985–1998. Br J Cancer 92, 188–193. [CrossRef] [Google Scholar]
  13. Dedicoat, M., Newton, R., Alkharsah, K. R., Sheldon, J., Szabados, I., Ndlovu, B., Page, T., Casabonne, D., Gilks, C. F. & other authors(2004). Mother-to-child transmission of human herpesvirus-8 in South Africa. J Infect Dis 190, 1068–1075. [CrossRef] [Google Scholar]
  14. Dukers, N. H. & Rezza, G.(2003). Human herpesvirus 8 epidemiology: what we do and do not know. AIDS 17, 1717–1730. [CrossRef] [Google Scholar]
  15. Guttman-Yassky, E., Kra-Oz, Z., Dubnov, J., Friedman-Birnbaum, R., Segal, I., Zaltzman, N., Roth, T., Schwartz, F., Linn, S. & other authors(2005). Infection with Kaposi's sarcoma-associated herpesvirus among families of patients with classic Kaposi's sarcoma. Arch Dermatol 141, 1429–1434. [Google Scholar]
  16. Hladik, W., Dollard, S. C., Mermin, J., Fowlkes, A. L., Downing, R., Amin, M. M., Banage, F., Nzaro, E., Kataaha, P. & other authors(2006). Transmission of human herpesvirus 8 by blood transfusion. N Engl J Med 355, 1331–1338. [CrossRef] [Google Scholar]
  17. Malope, B. I., Pfeiffer, R. M., Mbisa, G., Stein, L., Ratshikhopha, E. M., O'Connell, D. L., Sitas, F., MacPhail, P. & Whitby, D.(2007). Transmission of Kaposi sarcoma-associated herpesvirus between mothers and children in a South African population. J Acquir Immune Defic Syndr 44, 351–355. [CrossRef] [Google Scholar]
  18. Mancuso, R., Biffi, R., Valli, M., Bellinvia, M., Athanasia, T., Ferrucci, S., Brambilla, L., Delbue, S., Ferrante, P. & other authors(2008). HHV8 a subtype is associated with rapidly evolving classic Kaposi's sarcoma. J Med Virol 80, 2153–2160. [CrossRef] [Google Scholar]
  19. Marcelin, A. G., Gorin, I., Morand, P., Ait-Arkoub, Z., Deleuze, J., Morini, J. P., Calvez, V. & Dupin, N.(2004). Quantification of Kaposi's sarcoma-associated herpesvirus in blood, oral mucosa, and saliva in patients with Kaposi's sarcoma. AIDS Res Hum Retroviruses 20, 704–708. [CrossRef] [Google Scholar]
  20. Masala, M. V., Carcassi, C., Cottoni, F., Mulargia, M., Contu, L. & Cerimele, D.(2005). Classic Kaposi's sarcoma in Sardinia: HLA positive and negative associations. Int J Dermatol 44, 743–745. [CrossRef] [Google Scholar]
  21. Mbulaiteye, S. M., Pfeiffer, R. M., Engels, E. A., Marshall, V., Bakaki, P. M., Owor, A. M., Ndugwa, C. M., Katongole-Mbidde, E., Goedert, J. J. & other authors(2004). Detection of Kaposi sarcoma-associated herpesvirus DNA in saliva and buffy-coat samples from children with sickle cell disease in Uganda. J Infect Dis 190, 1382–1386. [CrossRef] [Google Scholar]
  22. Miyazawa, M., Lopalco, L., Mazzotta, F., Lo Caputo, S., Veas, F. & Clerici, M., ESN Study Group(2009). The ‘immunologic advantage’ of HIV-exposed seronegative individuals. AIDS 23, 161–175. [CrossRef] [Google Scholar]
  23. Nishimura, M., Kermode, A. G., Clerici, M., Shearer, G. M., Berzofsky, J. A., Uchiyama, T., Wiktor, S. Z., Plate, E., Maloney, B. & other authors(1994). Demonstration of human T lymphotropic virus type I (HTLV-I)-specific T cell responses from seronegative and polymerase chain reaction-negative persons exposed to HTLV-1. J Infect Dis 170, 334–338.[CrossRef] [Google Scholar]
  24. Pauk, J., Huang, M. L., Brodie, S. J., Wald, A., Koelle, D. M., Schacker, T., Celum, C., Selke, S. & Corey, L.(2000). Mucosal shedding of human herpesvirus 8 in men. N Engl J Med 343, 1369–1377. [CrossRef] [Google Scholar]
  25. Pellett, P. E., Wright, D. J., Engels, E. A., Ablashi, D. V., Dollard, S. C., Forghani, B., Glynn, S. A., Goedert, J. J., Jenkins, F. J. & other authors(2003). Multicenter comparison of serologic assays and estimation of human herpesvirus 8 seroprevalence among US blood donors. Transfusion 43, 1260–1268. [CrossRef] [Google Scholar]
  26. Plancoulaine, S., Abel, L., Trégouët, D., Duprez, R., van Beveren, M., Tortevoye, P., Froment, A. & Gessain, A.(2004). Respective roles of serological status and blood specific antihuman herpesvirus 8 antibody levels in human herpesvirus 8 intrafamilial transmission in a highly endemic area. Cancer Res 64, 8782–8787. [CrossRef] [Google Scholar]
  27. Rabkin, C. S., Schulz, T. F., Whitby, D., Lennette, E. T., Magpantay, L. I., Chatlynne, L. & Biggar, R. J.(1998). Interassay correlation of human herpesvirus 8 serologic tests. HHV-8 Interlaboratory Collaborative Group. J Infect Dis 178, 304–309.[CrossRef] [Google Scholar]
  28. Renne, R., Zhong, W., Herndier, B., McGrath, M., Abbey, N., Kedes, D. & Ganem, D.(1996). Lytic growth of Kaposi's sarcoma-associated herpesvirus (human herpesvirus 8) in culture. Nat Med 2, 342–346. [CrossRef] [Google Scholar]
  29. Schulz, T. F.(2000). Kaposi's sarcoma-associated herpesvirus (human herpesvirus 8): epidemiology and pathogenesis. J Antimicrob Chemother 45 (Suppl. T3) 15–27. [Google Scholar]
  30. Sitas, F. & Newton, R.(2001). Kaposi's sarcoma in South Africa. J Natl Cancer Inst Monogr 28, 1–4. [Google Scholar]
  31. Sosa, C., Benetucci, J., Hanna, C., Sieczkowski, L., Deluchi, G., Canizal, A. M., Mantina, H., Klaskala, W., Baum, M. & Wood, C.(2001). Human herpesvirus 8 can be transmitted through blood in drug addicts. Medicina (B Aires) 61, 291–294. [Google Scholar]
  32. Spira, T. J., Lam, L., Dollard, S. C., Meng, Y.-X., Pau, C. P., Black, J. B., Burns, D., Cooper, B., Hamid, M. & other authors(2000). Comparison of serologic assays and PCR for diagnosis of human herpesvirus 8 infection. J Clin Microbiol 38, 2174–2180. [Google Scholar]
  33. Tedeschi, R., De Paoli, P., Schulz, T. F. & Dillner, J.(1999). Human serum antibodies to a major defined epitope of human herpesvirus 8 small viral capsid antigen. J Infect Dis 179, 1016–1020. [CrossRef] [Google Scholar]
  34. Thompson, J. D., Higgins, D. G. & Gibson, T. J.(1994).clustalw: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22, 4673–4680. [CrossRef] [Google Scholar]
  35. Thompson, J. D., Gibson, T. J., Plewniak, F., Jeanmougin, F. & Higgins, D. G.(1997). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25, 4876–4882. [CrossRef] [Google Scholar]
  36. Vieira, J., Huang, M.-L., Koelle, D. M. & Corey, L.(1997). Transmissible Kaposi's sarcoma-associated herpesvirus (human herpesvirus 8) in saliva of men with a history of Kaposi's sarcoma. J Virol 71, 7083–7087. [Google Scholar]
  37. Vitale, F., Viviano, E., Perna, A. M., Bonura, F., Mazzola, G., Ajello, F. & Romano, N.(2000). Serological and virological evidence of non-sexual transmission of human herpesvirus type 8 (HHV8). Epidemiol Infect 125, 671–675. [CrossRef] [Google Scholar]
  38. Vitale, F., Briffa, D. V., Whitby, D., Maida, I., Grochowska, A., Levin, A., Romano, N. & Goedert, J. J.(2001). Kaposi's sarcoma herpes virus and Kaposi's sarcoma in the elderly populations of 3 Mediterranean islands. Int J Cancer 91, 588–591. [CrossRef] [Google Scholar]
  39. Whitby, D., Luppi, M., Barozzi, P., Boshoff, C., Weiss, R. A. & Torelli, G.(1998). Human herpesvirus 8 seroprevalence in blood donors and lymphoma patients from different regions of Italy. J Natl Cancer Inst 90, 395–397. [CrossRef] [Google Scholar]
  40. Whitby, D., Luppi, M., Sabin, C., Barozzi, P., Di Biase, A. R., Balli, F., Cucci, F., Weiss, R. A., Boshoff, C. & Torelli, G.(2000). Detection of antibodies to human herpesvirus 8 in Italian children: evidence for horizontal transmission. Br J Cancer 82, 702–704.[CrossRef] [Google Scholar]
  41. Zhu, L., Wang, R., Sweat, A., Goldstein, E., Horvat, R. & Chandran, B.(1999). Comparison of human sera reactivities in immunoblots with recombinant human herpesvirus (HHV)-8 proteins associated with the latent (ORF73) and lytic (ORFs 65, K8.1A, and K8.1B) replicative cycles and in immunofluorescence assays with HHV-8-infected BCBL-1 cells. Virology 256, 381–392. [CrossRef] [Google Scholar]
  42. Zong, J.-C., Ciufo, D. M., Alcendor, D. J., Wan, X., Nicholas, J., Browning, P. J., Rady, P. L., Tyring, S. K., Orenstein, J. M. & other authors(1999). High-level variability in the ORF-K1 membrane protein gene at the left end of the Kaposi's sarcoma-associated herpesvirus genome defines four major virus subtypes and multiple variants or clades in different human populations. J Virol 73, 4156–4170. [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/vir.0.027847-0
Loading
Intrafamiliar transmission of Kaposi's sarcoma-associated herpesvirus and seronegative infection in family members of classic Kaposi's sarcoma patients
J. Gen. Virol. 92, 744 (2011); https://doi.org/10.1099/vir.0.027847-0
/content/journal/jgv/10.1099/vir.0.027847-0
/content/journal/jgv/10.1099/vir.0.027847-0
Loading

Data & Media loading...

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error