1887

Abstract

We have used high-throughput Illumina sequencing to identify novel recombinants between (DWV) and (VDV-1), which accumulate to higher levels than DWV in both honeybees and mites. The recombinants, VDV-1 and VDV-1, exhibit crossovers between the 5′-UTR and the regions encoding the structural (capsid) and non-structural viral proteins. This implies that the genomes are modular and that each region may evolve independently, as demonstrated in human enteroviruses. Individual honeybee pupae were infected with a mixture of observed recombinants and DWV. A strong correlation was observed between VDV-1 levels in honeybee pupae and associated mites, suggesting that this recombinant, with a DWV-derived 5′-UTR and non-structural protein region flanking a VDV-1-derived capsid-encoding region, is better adapted to transmission between and honeybees than the parental DWV or a recombinant bearing the VDV-1-derived 5′-UTR (VDV-1).

Loading

Article metrics loading...

/content/journal/jgv/10.1099/vir.0.025965-0
2011-01-01
2019-11-20
Loading full text...

Full text loading...

/deliver/fulltext/jgv/92/1/156.html?itemId=/content/journal/jgv/10.1099/vir.0.025965-0&mimeType=html&fmt=ahah

References

  1. Chen, Y. P. & Siede, R. ( 2007; ). Honeybee viruses. Adv Virus Res 70, 33–80.
    [Google Scholar]
  2. de Miranda, J. R. & Genersch, E. ( 2010; ). Deformed wing virus. J Invertebr Pathol 103, S48–S61.[CrossRef]
    [Google Scholar]
  3. Fujiyuki, T., Takeuchi, H., Ono, M., Ohka, S., Sasaki, T., Nomoto, A. & Kubo, T. ( 2004; ). Novel insect picorna-like virus identified in the brains of aggressive worker honeybees. J Virol 78, 1093–1100.[CrossRef]
    [Google Scholar]
  4. Gisder, S., Aumeier, P. & Genersch, E. ( 2009; ). Deformed wing virus: replication and viral load in mites (Varroa destructor). J Gen Virol 90, 463–467.[CrossRef]
    [Google Scholar]
  5. Highfield, A. C., El Nagar, A., Mackinder, L. C., Noël, L. M., Hall, M. J., Martin, S. J. & Schroeder, D. C. ( 2009; ). Deformed wing virus implicated in overwintering honeybee colony losses. Appl Environ Microbiol 75, 7212–7220.[CrossRef]
    [Google Scholar]
  6. Jay, S. C. ( 1962; ). Colour changes in honeybee pupae. Bee World 43, 119–122.
    [Google Scholar]
  7. Koonin, E. V. & Dolja, V. V. ( 1993; ). Evolution and taxonomy of positive-strand RNA viruses: implications of comparative analysis of amino acid sequences. Crit Rev Biochem Mol Biol 28, 375–430.[CrossRef]
    [Google Scholar]
  8. Langmead, B., Trapnell, C., Pop, M. & Salzberg, S. L. ( 2009; ). Ultrafast and memory-efficient alignment of short DNA sequences to the human genome. Genome Biol 10, R25.[CrossRef]
    [Google Scholar]
  9. Lanzi, G., de Miranda, J. R., Boniotti, M. B., Cameron, C. E., Lavazza, A., Capucci, L., Camazine, S. M. & Rossi, C. ( 2006; ). Molecular and biological characterization of deformed wing virus of honeybees (Apis mellifera L.). J Virol 80, 4998–5009.[CrossRef]
    [Google Scholar]
  10. Lole, K. S., Bollinger, R. C., Paranjape, R. S., Gadkari, D., Kulkarni, S. S., Novak, N. G., Ingersoll, R., Sheppard, H. W. & Ray, S. C. ( 1999; ). Full-length human immunodeficiency virus type 1 genomes from subtype C-infected seroconverters in India, with evidence of intersubtype recombination. J Virol 73, 152–160.
    [Google Scholar]
  11. Lukashev, A. N. ( 2005; ). Role of recombination in evolution of enteroviruses. Rev Med Virol 15, 157–167.[CrossRef]
    [Google Scholar]
  12. Lukashev, A. N., Lashkevich, V. A., Ivanova, O. E., Koroleva, G. A., Hinkkanen, A. E. & Ilonen, J. ( 2005; ). Recombination in circulating Human enterovirus B: independent evolution of structural and non-structural genome regions. J Gen Virol 86, 3281–3290.[CrossRef]
    [Google Scholar]
  13. McWilliam Leitch, E. C., Bendig, J., Cabrerizo, M., Cardosa, J., Hyypiä, T., Ivanova, O. E., Kelly, A., Kroes, A. C., Lukashev, A. & other authors ( 2009; ). Transmission networks and population turnover of echovirus 30. J Virol 83, 2109–2118.[CrossRef]
    [Google Scholar]
  14. McWilliam Leitch, E. C., Cabrerizo, M., Cardosa, J., Harvala, H., Ivanova, O. E., Kroes, A. C., Lukashev, A., Muir, P., Odoom, J. & other authors ( 2010; ). Evolutionary dynamics and temporal/geographical correlates of recombination in the human enterovirus echovirus types 9, 11, and 30. J Virol 84, 9292–9300.[CrossRef]
    [Google Scholar]
  15. Oberste, M. S., Maher, K. & Pallansch, M. A. ( 2004; ). Evidence for frequent recombination within species human enterovirus B based on complete genomic sequences of all thirty-seven serotypes. J Virol 78, 855–867.[CrossRef]
    [Google Scholar]
  16. Ongus, J. R., Peters, D., Bonmatin, J. M., Bengsch, E., Vlak, J. M. & van Oers, M. M. ( 2004; ). Complete sequence of a picorna-like virus of the genus Iflavirus replicating in the mite Varroa destructor. J Gen Virol 85, 3747–3755.[CrossRef]
    [Google Scholar]
  17. Ongus, J. R., Roode, E. C., Pleij, C. W., Vlak, J. M. & van Oers, M. M. ( 2006; ). The 5′ non-translated region of Varroa destructor virus 1 (genus Iflavirus): structure prediction and IRES activity in Lymantria dispar cells. J Gen Virol 87, 3397–3407.[CrossRef]
    [Google Scholar]
  18. Ryabov, E. V., Keane, G., Naish, N., Evered, C. & Winstanley, D. ( 2009; ). Densovirus induces winged morphs in asexual clones of the rosy apple aphid, Dysaphis plantaginea. Proc Natl Acad Sci U S A 106, 8465–8470.[CrossRef]
    [Google Scholar]
  19. Santillán-Galicia, M. T., Carzaniga, R., Ball, B. V. & Alderson, P. G. ( 2008; ). Immunolocalization of deformed wing virus particles within the mite Varroa destructor. J Gen Virol 89, 1685–1689.[CrossRef]
    [Google Scholar]
  20. Simmonds, P. ( 2006; ). Recombination and selection in the evolution of picornaviruses and other mammalian positive-stranded RNA viruses. J Virol 80, 11124–11140.[CrossRef]
    [Google Scholar]
  21. van Rij, R. P. & Berezikov, E. ( 2009; ). Small RNAs and the control of transposons and viruses in Drosophila. Trends Microbiol 17, 163–171.[CrossRef]
    [Google Scholar]
  22. Yang, X. & Cox-Foster, D. L. ( 2005; ). Impact of an ectoparasite on the immunity and pathology of an invertebrate: evidence for host immunosuppression and viral amplification. Proc Natl Acad Sci U S A 102, 7470–7475.[CrossRef]
    [Google Scholar]
  23. Yue, C. & Genersch, E. ( 2005; ). RT-PCR analysis of Deformed wing virus in honeybees (Apis mellifera) and mites (Varroa destructor). J Gen Virol 86, 3419–3424.[CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/vir.0.025965-0
Loading
/content/journal/jgv/10.1099/vir.0.025965-0
Loading

Data & Media loading...

Supplements

vol. , part 1, pp. 156 - 161

Oligonucleotides used in this study. [PDF](53 KB)



PDF

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error