In order to identify the transcriptional units expressed from an entire nucleopolyhedrovirus (NPV) genome during infection, we constructed a full-length-enriched cDNA library from NPV (BmNPV)-infected BmN cells. We randomly sequenced 11 520 clones from both ends to obtain a total of 4679 BmNPV-derived transcriptional units. The data revealed a number of novel transcripts, including putative non-coding RNAs, most of which are expressed from recognized baculovirus early or late promoter motifs. These findings provide new insights into the complex transcriptional regulation of an NPV genome and suggest roles for as-yet-uncharacterized transcripts.


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Transcriptional units identified in BmNPV-infected BmN cells. [PDF](491 KB)


Putative ncRNAs (56 transcripts) expressed from the BmNPV genome. [PDF](147 KB)

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