1887

Abstract

In order to identify the transcriptional units expressed from an entire nucleopolyhedrovirus (NPV) genome during infection, we constructed a full-length-enriched cDNA library from NPV (BmNPV)-infected BmN cells. We randomly sequenced 11 520 clones from both ends to obtain a total of 4679 BmNPV-derived transcriptional units. The data revealed a number of novel transcripts, including putative non-coding RNAs, most of which are expressed from recognized baculovirus early or late promoter motifs. These findings provide new insights into the complex transcriptional regulation of an NPV genome and suggest roles for as-yet-uncharacterized transcripts.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/vir.0.025908-0
2011-01-01
2020-01-24
Loading full text...

Full text loading...

/deliver/fulltext/jgv/92/1/200.html?itemId=/content/journal/jgv/10.1099/vir.0.025908-0&mimeType=html&fmt=ahah

References

  1. Dean, F. B., Nelson, J. R., Giesler, T. L. & Lasken, R. S. ( 2001; ). Rapid amplification of plasmid and phage DNA using Phi29 DNA polymerase and multiply-primed rolling circle amplification. Genome Res 11, 1095–1099.[CrossRef]
    [Google Scholar]
  2. Happ, B., Li, J. & Doerfler, W. ( 1991; ). Proteins encoded in the 81.2- to 85.0-map-unit fragment of Autographa californica nuclear polyhedrosis virus DNA can be translated in vitro and in Spodoptera frugiperda cells. J Virol 65, 89–97.
    [Google Scholar]
  3. Huang, J., Hao, B., Deng, F., Sun, X., Wang, H. & Hu, Z. ( 2008; ). Open reading frame Bm21 of Bombyx mori nucleopolyhedrovirus is not essential for virus replication in vitro, but its deletion extends the median survival time of infected larvae. J Gen Virol 89, 922–930.[CrossRef]
    [Google Scholar]
  4. Iwanaga, M., Takaya, K., Katsuma, S., Ote, M., Tanaka, S., Kamita, S. G., Kang, W., Shimada, T. & Kobayashi, M. ( 2004; ). Expression profiling of baculovirus genes in permissive and nonpermissive cell lines. Biochem Biophys Res Commun 323, 599–614.[CrossRef]
    [Google Scholar]
  5. Jiang, S. S., Chang, I. S., Huang, L. W., Chen, P. C., Wen, C. C., Liu, S. C., Chien, L. C., Lin, C. Y., Hsiung, C. A. & Juang, J. L. ( 2006; ). Temporal transcription program of recombinant Autographa californica multiple nucleopolyhedrosis virus. J Virol 80, 8989–8999.[CrossRef]
    [Google Scholar]
  6. Kato, S., Ohtoko, K., Ohtake, H. & Kimura, T. ( 2005; ). Vector-capping: a simple method for preparing a high-quality full-length cDNA library. DNA Res 12, 53–62.[CrossRef]
    [Google Scholar]
  7. Kikhno, I., Gutiérrez, S., Croizier, L., Croizier, G. & Ferber, M. L. ( 2002; ). Characterization of pif, a gene required for the per os infectivity of Spodoptera littoralis nucleopolyhedrovirus. J Gen Virol 83, 3013–3022.
    [Google Scholar]
  8. Ooi, B. G. & Miller, L. K. ( 1990; ). Transcription of the baculovirus polyhedrin gene reduces the levels of an antisense transcript initiated downstream. J Virol 64, 3126–3129.
    [Google Scholar]
  9. Smith, I. ( 2007; ). Misleading messengers? Interpreting baculovirus transcriptional array profiles. J Virol 81, 7819–7820.[CrossRef]
    [Google Scholar]
  10. Yamagishi, J., Isobe, R., Takebuchi, T. & Bando, H. ( 2003; ). DNA microarrays of baculovirus genomes: differential expression of viral genes in two susceptible insect cell lines. Arch Virol 148, 587–597.[CrossRef]
    [Google Scholar]
  11. Yang, D. H., Barari, M., Arif, B. M. & Krell, P. J. ( 2007; ). Development of an oligonucleotide-based DNA microarray for transcriptional analysis of Choristoneura fumiferana nucleopolyhedrovirus (CfMNPV) genes. J Virol Methods 143, 175–185.[CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/vir.0.025908-0
Loading
/content/journal/jgv/10.1099/vir.0.025908-0
Loading

Data & Media loading...

Supplements

Transcriptional units identified in BmNPV-infected BmN cells. [PDF](491 KB)

PDF

Putative ncRNAs (56 transcripts) expressed from the BmNPV genome. [PDF](147 KB)

PDF

Most cited articles

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error