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Volume 91, Issue 12

Mycoviruses related to chrysovirus affect vegetative growth in the rice blast fungus Free

Abstract

Mycoviruses causing impaired growth and abnormal pigmentation of the host were found in the rice blast fungus, . Four dsRNAs, dsRNA 1 (3554 bp), dsRNA 2 (3250 bp), dsRNA 3 (3074 bp) and dsRNA 4 (3043 bp), were detected in isolate S-0412-II 1a of . By picking up single conidia of S-0412-II 1a, cured strains of the fungus were isolated that had completely lost the mycovirus. The cured strains had normal mycelial growth and pigmentation, suggesting that this mycovirus modulates host traits. The buoyant densities of isometric virus particles (∼35 nm diameter) containing these dsRNAs in CsCl ranged from 1.37 to 1.40 g cm. The single ORF (3384 nt) of dsRNA 1 encoded a gene product highly homologous to the viral RNA-dependent RNA polymerase of members of the family . It is noteworthy that mycovirus S-0412-II 1a was detected not only in host cells but also in culture supernatant. Furthermore, abnormal aggregation of mycelia was observed after adding the mycovirus-containing culture supernatant to an uninfected strain of and mycoviral dsRNAs were detectable from the aggregated mycelia. This novel dsRNA mycovirus was named chrysovirus 1.

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2010-12-01
2024-04-19
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Mycoviruses related to chrysovirus affect vegetative growth in the rice blast fungus Magnaporthe oryzae
J. Gen. Virol. 91, 3085 (2010); https://doi.org/10.1099/vir.0.025411-0
/content/journal/jgv/10.1099/vir.0.025411-0
/content/journal/jgv/10.1099/vir.0.025411-0
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vol. , part 12, pp. 3085 - 3094

GenBank/protein accession numbers for the phylogenetic analysis of the RdRp sequences of MoCV1 and 31 other selected viruses [PDF](13 KB)

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