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Volume 92, Issue 2

Thermal transition of native tobacco mosaic virus and RNA-free viral proteins into spherical nanoparticles Free

Abstract

Spherical nanoparticles (SNPs) were generated by two-step thermal remodelling of native tobacco mosaic virus (TMV) at 94 °C. Particles of irregular shape and varying size were generated by TMV at 90 °C. They could be converted into SNPs by heating at 94 °C and were considered to be intermediate precursors of SNPs. In addition to SNP monomers (53 nm diameter), generated by individual TMV virions, large SNPs (100–800 nm diameter) were assembled. The size of the SNPs depended on the TMV concentration. The SNPs could be generated by distinct forms of RNA-free TMV coat protein (CP) aggregates and individual CP subunits. A one-step SNP assembly appeared to occur in these cases. These results show that SNPs represent a new type of particle nanoplatform for producing compositions of SNPs with foreign protein molecules bound to their surface.

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  • Accepted:
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2011-02-01
2024-04-19
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References

  1. Anderer, F. A.(1959). Das molekulargemicht der peptide inheit im protein des Tabakmosaikvirus. Z Naturforsch B 14, 24–28 (in German ). [Google Scholar]
  2. Anderer, F. A.(1963). Recent studies on the structure of tobacco mosaic virus. Adv Protein Chem 18, 1–35. [Google Scholar]
  3. Butler, P. J. G.(1999). Self-assembly of tobacco mosaic virus: the role of intermediate aggregate in generating both specificity and speed. Philos Trans R Soc Lond B Biol Sci 354, 537–550.[CrossRef] [Google Scholar]
  4. Butler, P. J. J. & Klug, A.(1978). The assembly of a virus. Sci Am 239, 62–69. [Google Scholar]
  5. Caspar, D. L. D.(1963). Assembly and stability of the tobacco mosaic virus particle. Adv Protein Chem 18, 37–121. [Google Scholar]
  6. Díaz-Avalos, R. & Caspar, D. L.(1998). Structure of the stacked disk aggregate of tobacco mosaic virus protein. Biophys J 74, 595–603.[CrossRef] [Google Scholar]
  7. Fraenkel-Conrat, H. & Singer, B.(1999). Virus reconstitution and the proof of the existence of the genomic RNA. Philos Trans R Soc Lond B Biol Sci 354, 583–586.[CrossRef] [Google Scholar]
  8. Hart, R. G.(1956). Morphological changes accompanying thermal denaturation of tobacco mosaic virus. Biochim Biophys Acta 20, 388–389.[CrossRef] [Google Scholar]
  9. Klug, A.(1999). The tobacco mosaic virus particle: structure and assembly. Philos Trans R Soc Lond B Biol Sci 354, 531–535.[CrossRef] [Google Scholar]
  10. Lauffer, M. A. & Price, W. C.(1940). Thermal denaturation of tobacco mosaic virus. J Biol Chem 133, 1–15. [Google Scholar]
  11. Lauffer, M. A. & Stevens, C. L.(1968). Structure of the tobacco mosaic virus particle: polymerization of tobacco mosaic virus protein. Adv Virus Res 13, 1–63. [Google Scholar]
  12. Namba, K., Pattanayek, R. & Stubbs, G.(1989). Visualization of protein–nucleic acid interactions in a virus. Refined structure of intact tobacco mosaic virus at 2.9 Å resolution by X-ray fiber diffraction. J Mol Biol 208, 307–325.[CrossRef] [Google Scholar]
  13. Schramm, G. & Zillig, W.(1955). Über die Struktur des Tabakmosaikvirus. IV. Mitt.: Die Reaggregation des nucleinsäure-freinen Proteins. Z Naturforsch B 10, 493–499 (in German). [Google Scholar]
  14. Wittmann, H. G.(1959). Darstellung und physikochemiche Peptidketten des Tabakmosaikvirus. Experientia 15, 174–175 (in German ).[CrossRef] [Google Scholar]
  15. Zaitlin, M. & Israel, H. W.(1975). Tobacco mosaic virus (type strain). CMI/AAB Descriptions of Plant Viruses, No. 151.
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Thermal transition of native tobacco mosaic virus and RNA-free viral proteins into spherical nanoparticles
J. Gen. Virol. 92, 453 (2011); https://doi.org/10.1099/vir.0.024356-0
/content/journal/jgv/10.1099/vir.0.024356-0
/content/journal/jgv/10.1099/vir.0.024356-0
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vol. , part 2, pp. 453 - 456

A single protein band with the mobility of TMV CP was revealed by SDS-PAGE of CP isolated from native virus and SNPs

Comparison of the antigenic specificity of native TMV and SNPs by TAS-ELISA [Single PDF file](189 KB)

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