Complete genome sequences were determined for two distinct strains of slow bee paralysis virus (SBPV) of honeybees (). The SBPV genome is approximately 9.5 kb long and contains a single ORF flanked by 5′- and 3′-UTRs and a naturally polyadenylated 3′ tail, with a genome organization typical of members of the family . The two strains, labelled ‘Rothamsted’ and ‘Harpenden’, are 83 % identical at the nucleotide level (94 % identical at the amino acid level), although this variation is distributed unevenly over the genome. The two strains were found to co-exist at different proportions in two independently propagated SBPV preparations. The natural prevalence of SBPV for 847 colonies in 162 apiaries across five European countries was <2 %, with positive samples found only in England and Switzerland, in colonies with variable degrees of infestation.


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vol. , part 10, pp. 2524–2530

Primers and performance indicators of several universal and strain-specific RT-qPCR assays [ PDF] (73 KB)

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