@article{mbs:/content/journal/jgv/10.1099/vir.0.022418-0, author = "Miyamura, Kohei and Nishimura, Yorihiro and Abo, Masahiro and Wakita, Takaji and Shimizu, Hiroyuki", title = "Adaptive mutations in the genomes of enterovirus 71 strains following infection of mouse cells expressing human P-selectin glycoprotein ligand-1", journal= "Journal of General Virology", year = "2011", volume = "92", number = "2", pages = "287-291", doi = "https://doi.org/10.1099/vir.0.022418-0", url = "https://www.microbiologyresearch.org/content/journal/jgv/10.1099/vir.0.022418-0", publisher = "Microbiology Society", issn = "1465-2099", type = "Journal Article", abstract = "We recently identified human P-selectin glycoprotein ligand-1 (PSGL-1) as a functional enterovirus 71 (EV71) receptor and demonstrated PSGL-1-dependent replication for some EV71 strains in human leukocytes. Here, we report that four out of five PSGL-1-binding strains of EV71 replicated poorly in mouse L929 cells stably expressing human PSGL-1 (L-PSGL-1 cells). Therefore, we compared the replication kinetics and entire genomic sequence of five original EV71 strains and the corresponding EV71 variants (EV71-LPS), which were propagated once in L-PSGL-1 cells. Direct sequence comparison of the entire genome of the original EV71 strains and EV71-LPS variants identified several possible adaptive mutations during the course of replication in L-PSGL-1 cells, including a putative determinant of the adaptive phenotype in L-PSGL-1 cells at VP2-149. The results suggest that an adaptive mutation, along with a PSGL-1-binding phenotype, may facilitate efficient PSGL-1-dependent replication of the EV71 strains in L-PSGL-1 cells.", }