%0 Journal Article %A Soowannayan, Chumporn %A Cowley, Jeff A. %A Pearson, Roger D. %A Wallis, Tristan P. %A Gorman, Jeffrey J. %A Michalski, Wojtek P. %A Walker, Peter J. %T Glycosylation of gp116 and gp64 envelope proteins of yellow head virus of Penaeus monodon shrimp %D 2010 %J Journal of General Virology, %V 91 %N 10 %P 2463-2473 %@ 1465-2099 %R https://doi.org/10.1099/vir.0.022111-0 %I Microbiology Society, %X Yellow head virus (YHV) is a highly virulent pathogen of Penaeus monodon shrimp that is classified in the genus Okavirus, family Roniviridae, in the order Nidovirales. Separation of virion proteins treated with peptide-N-glycosidase-F (PNGase-F) in SDS-polyacrylamide gels and the use of glycoprotein-specific staining methods indicated that the gp116 and gp64 envelope glycoproteins possess N-linked rather than O-linked glycans. Competitive binding inhibition of lectins with various oligosaccharide specificities indicated that glycans linked to gp64 are mannose-rich, whilst glycans linked to gp116 possess terminal N-acetylgalactosamine and N-acetylglucosamine in addition to terminal mannose-type sugars. Mass spectrometry analyses of peptides generated from YHV proteins before and after deglycosylation with PNGase-F, using combinations of the endoproteinases trypsin, Asp-N and Lys-C, confirmed occupancy of six of the seven potential N-linked glycosylation sites in gp116 and three of the four potential sites in gp64. %U https://www.microbiologyresearch.org/content/journal/jgv/10.1099/vir.0.022111-0