Yellow head virus (YHV) is a highly virulent pathogen of shrimp that is classified in the genus , family , in the order . Separation of virion proteins treated with peptide--glycosidase-F (PNGase-F) in SDS-polyacrylamide gels and the use of glycoprotein-specific staining methods indicated that the gp116 and gp64 envelope glycoproteins possess -linked rather than -linked glycans. Competitive binding inhibition of lectins with various oligosaccharide specificities indicated that glycans linked to gp64 are mannose-rich, whilst glycans linked to gp116 possess terminal -acetylgalactosamine and -acetylglucosamine in addition to terminal mannose-type sugars. Mass spectrometry analyses of peptides generated from YHV proteins before and after deglycosylation with PNGase-F, using combinations of the endoproteinases trypsin, Asp-N and LysC, confirmed occupancy of six of the seven potential linked glycosylation sites in gp116 and three of the four potential sites in gp64.


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vol. , part 10, pp. 2463–2473

Blotting of human blood type O erythrocyte membrane proteins with lectins, including:

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