1887

Abstract

The placenta is important in the horizontal transmission of the aetiological agent in scrapie-affected sheep. It has been demonstrated that the placentas of fetuses carrying the dimorphism Q171R of the gene is resistant to pathological prion protein (PrP) accumulation in the placenta. To test whether other polymorphisms are associated with a lack of placental PrP deposition, we carried out a study on 26 naturally and 11 experimentally scrapie-affected ewes with or without clinical signs. PrP was detected in the placenta of ARQ/ARQ fetuses by Western blot and immunohistochemical analysis, but not in ARQN/ARQK or, as expected, ARQ/ARR samples. Furthermore, three of four ALRQ/AFRQ placentas were also PrP negative, suggesting that the dimorphism at codon 141 may also mediate placental deposition of PrP. This finding demonstrates for the first time that fetal polymorphisms, other than those at codon 171, are associated with the lack of placental deposition of PrP.

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2010-09-01
2019-11-12
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References

  1. Alverson, J., O'Rourke, K. I. & Baszler, T. V. ( 2006; ). PrPSc accumulation in fetal cotyledons of scrapie-resistant lambs is influenced by fetus location in the uterus. J Gen Virol 87, 1035–1041.[CrossRef]
    [Google Scholar]
  2. Andréoletti, O., Lacroux, C., Chabert, A., Monnereau, L., Tabouret, G., Lantier, F., Berthon, P., Eychenne, F., Lafond-Benestad, S. & other authors ( 2002; ). PrPSc accumulation in placentas of ewes exposed to natural scrapie: influence of foetal genotype and effect on ewe-to-lamb transmission. J Gen Virol 83, 2607–2616.
    [Google Scholar]
  3. Basler, K., Oesch, B., Scott, M., Westaway, D., Wälchli, M., Groth, D. F., McKinley, M. P., Prusiner, S. B. & Weissmann, C. ( 1986; ). Scrapie and cellular PrP isoforms are encoded by the same chromosomal gene. Cell 46, 417–428.[CrossRef]
    [Google Scholar]
  4. Caplazi, P., O'Rourke, K., Wolf, C., Shaw, D. & Baszler, T. V. ( 2004; ). Biology of PrPSc accumulation in two natural scrapie-infected sheep flocks. J Vet Diagn Invest 16, 489–496.[CrossRef]
    [Google Scholar]
  5. Ersdal, C., Ulvund, M. J., Espenes, A., Benestad, S. L., Sarradin, P. & Landsverk, T. ( 2005; ). Mapping PrPSc propagation in experimental and natural scrapie in sheep with different PrP genotypes. Vet Pathol 42, 258–274.[CrossRef]
    [Google Scholar]
  6. Goldmann, W., Baylis, M., Chihota, C., Stevenson, E. & Hunter, N. ( 2005; ). Frequencies of PrP gene haplotypes in British sheep flocks and the implications from breeding programmes. J Appl Microbiol 98, 1294–1302.[CrossRef]
    [Google Scholar]
  7. Goldmann, W., Houston, F., Stewart, P., Perucchini, M., Foster, J. & Hunter, N. ( 2006; ). Ovine prion protein variant A(136) R(154) L(168) Q(171) increases resistance to experimental challenge with bovine spongiform encephalopathy agent. J Gen Virol 87, 3741–3745.[CrossRef]
    [Google Scholar]
  8. Hunter, N. ( 1997; ). PrP genetics in sheep and the implications for scrapie and BSE. Trends Microbiol 5, 331–334.[CrossRef]
    [Google Scholar]
  9. Lacroux, C., Corbière, F., Tabouret, G., Lugan, S., Costes, P., Mathey, J., Delmas, J. M., Weisbecker, J. L., Foucras, G. & other authors ( 2007; ). Dynamics and genetics of PrPSc placental accumulation in sheep. J Gen Virol 88, 1056–1061.[CrossRef]
    [Google Scholar]
  10. Laegreid, W. W., Clawson, M. L., Heaton, M. P., Green, B. T., O'Rourke, K. I. & Knowles, D. P. ( 2008; ). Scrapie resistance in ARQ sheep. J Virol 82, 10318–10320.[CrossRef]
    [Google Scholar]
  11. Ligios, C., Cancedda, M. G., Madau, L., Santucciu, C., Maestrale, C., Agrimi, U., Ru, G. & Di Guardo, G. ( 2006; ). PrPSc deposition in nervous tissues without lymphoid tissue involvement is frequently found in ARQ/ARQ Sarda breed sheep preclinically affected with natural scrapie. Arch Virol 151, 2007–2020.[CrossRef]
    [Google Scholar]
  12. Maestrale, C., Carta, A., Attene, S., Galistu, A., Santucciu, C., Cancedda, M. G., Saba, M., Sechi, S., Patta, C. & other authors ( 2009; ). p.Asn176Lys and p.Met137Thr dimorphisms of the PRNP gene significantly decrease the susceptibility to classical scrapie in ARQ/ARQ sheep. Anim Genet 40, 982–985.[CrossRef]
    [Google Scholar]
  13. Moum, T., Olsaker, I., Hopp, P., Moldal, T. K., Valheim, M., Moum, T. & Benestad, S. L. ( 2005; ). Polymorphisms at codons 141 and 154 in the ovine prion protein gene are associated with scrapie Nor98 cases. J Gen Virol 86, 231–235.[CrossRef]
    [Google Scholar]
  14. Saunders, G. C., Lantier, I., Cawthraw, S., Berthon, P., Moore, S. J., Arnold, M., Windl, O., Simmons, M. M., Andréoletti, O. & other authors ( 2009; ). Protective effect of the T112 PrP variant in sheep challenged with BSE. J Gen Virol 90, 2569–2574.[CrossRef]
    [Google Scholar]
  15. Tuo, W., O'Rourke, K. I., Zhuang, D., Cheevers, W. P., Spraker, T. R. & Knowles, D. P. ( 2002; ). Pregnancy status and fetal prion genetics determine PrPSc accumulation in placentomes of scrapie-infected sheep. Proc Natl Acad Sci U S A 99, 6310–6315.[CrossRef]
    [Google Scholar]
  16. Vaccari, G., Petraroli, R., Agrimi, U., Eleni, C., Perfetti, M. G., Di Bari, M. A., Morelli, L., Ligios, C., Butani, L. & other authors ( 2001; ). PrP genotype in Sarda breed sheep and its relevance to scrapie. Arch Virol 146, 2029–2037.[CrossRef]
    [Google Scholar]
  17. Vaccari, G., D'Agostino, C., Nonno, R., Rosone, F., Conte, M., Di Bari, M. A., Chiappini, B., Esposito, E., De Grossi, L. & other authors ( 2007; ). Prion protein alleles showing a protective effect on the susceptibility of sheep to scrapie and bovine spongiform encephalopathy. J Virol 81, 7306–7309.[CrossRef]
    [Google Scholar]
  18. Vaccari, G., Scavia, G., Sala, M., Cosseddu, G., Chiappini, B., Conte, M., Esposito, E., Lorenzetti, R., Perfetti, G. & other authors ( 2009; ). Protective effect of AT137RQ and ARQK176 PrP allele against classical scrapie in Sarda breed sheep. Vet Res 40, 19 [CrossRef]
    [Google Scholar]
  19. Wells, G. A. H. & McGill, I. S. ( 1992; ). Recently described scrapie-like encephalopathies of animals: case definitions. Res Vet Sci 53, 1–10.[CrossRef]
    [Google Scholar]
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vol. , part 9, pp. 2402 - 2407

Quantification of PrP in ARQ/ARQ and AL RQ/AF RQ placental homogenates from the twin pregnancy [PDF](253 KB)



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