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Journal of General Virology header logo Journal of General Virology

Volume 91, Issue 9

Cytoplasmic tails of hantavirus glycoproteins interact with the nucleocapsid protein No Access

Abstract

Here we characterize the interaction between the glycoproteins (Gn and Gc) and the ribonucleoprotein (RNP) of Puumala virus (PUUV; genus , family ). The interaction was initially established with native proteins by co-immunoprecipitating PUUV nucleocapsid (N) protein with the glycoprotein complex. Mapping of the interaction sites revealed that the N protein has multiple binding sites in the cytoplasmic tail (CT) of Gn and is also able to bind to the predicted CT of Gc. The importance of Gn- and Gc-CTs to the recognition of RNP was further verified in pull-down assays using soluble peptides with binding capacity to both recombinant N protein and the RNPs of PUUV and Tula virus. Additionally, the N protein of PUUV was demonstrated to interact with peptides of Gn and Gc from a variety of hantavirus species, suggesting a conserved RNP-recognition mechanism within the genus. Based on these and our previous results, we suggest that the complete hetero-oligomeric (Gn–Gc) spike complex of hantaviruses mediates the packaging of RNP into virions.

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2010-09-01
2025-02-09
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Cytoplasmic tails of hantavirus glycoproteins interact with the nucleocapsid protein
J. Gen. Virol. 91, 2341 (2010); https://doi.org/10.1099/vir.0.021006-0
/content/journal/jgv/10.1099/vir.0.021006-0
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vol. , part 9, pp. 2341–2350

(a) BacN binding to Gc-CT of PUUV. (b) RNP binding to Gc-CT of PUUV.

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