RT Journal Article SR Electronic(1) A1 Lai, Jimmy C. C. A1 Chan, Wallace W. L. A1 Kien, François A1 Nicholls, John M. A1 Peiris, J. S. Malik A1 Garcia, Jean-MichelYR 2010 T1 Formation of virus-like particles from human cell lines exclusively expressing influenza neuraminidase JF Journal of General Virology, VO 91 IS 9 SP 2322 OP 2330 DO https://doi.org/10.1099/vir.0.019935-0 PB Microbiology Society, SN 1465-2099, AB The minimal virus requirements for the generation of influenza virus-like particle (VLP) assembly and budding were reassessed. Using neuraminidase (NA) from the H5N1 and H1N1 subtypes, it was found that the expression of NA alone was sufficient to generate and release VLPs. Biochemical and functional characterization of the NA-containing VLPs demonstrated that they were morphologically similar to influenza virions. The NA oligomerization was comparable to that of the live virus, and the enzymic activity, whilst not required for the release of NA-VLPs, was preserved. Together, these findings indicate that NA plays a key role in virus budding and morphogenesis, and demonstrate that NA-VLPs represent a useful tool in influenza research., UL https://www.microbiologyresearch.org/content/journal/jgv/10.1099/vir.0.019935-0