Genomic heterogeneity and quasispecies composition of GB virus C (GBV-C) within plasma and lymphocyte subsets in a naturally infected blood donor were investigated. For this purpose, fragments from the 5′ untranslated region (5′ UTR) and the E2 gene recovered from plasma, B and T lymphocytes, were cloned and sequenced. A total of 63 clones was analysed: 95.2 % of them (=60) – obtained from plasma and cells – were assigned to genotype 2b, while only three derived from plasma corresponded to genotyope 3. The G215A transition within this region was present in 90.9 % of the clones from B lymphocytes, but absent in the remaining cell compartments (<0.01). Apparently, most of the circulating GBV-C quasispecies in this blood donor were related to the viral population infecting CD8 T cells, and B cells to a lesser extent. This is the first report showing the quasispecies nature of GBV-C in lymphocyte subsets within peripheral blood mononuclear cells.


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  1. Alter, H. J.(1997). G-pers creepers, where’d you get those papers? A reassessment of the literature on the hepatitis G virus. Transfusion 37, 569–572.[CrossRef] [Google Scholar]
  2. Fogeda, M., Navas, S., Martin, J., Casqueiro, M., Rodríguez, E., Arocena, C. & Carreño, V.(1999).In vitro infection of human peripheral blood mononuclear cells by GB virus C/hepatitis G virus. J Virol 73, 4052–4061. [Google Scholar]
  3. Fogeda, M., López-Alcorocho, J. M., Bartolomé, J., Arocena, C., Martín, M. A. & Carreño, V.(2000). Existence of distinct GB virus C/Hepatitis G virus variants with different tropism. J Virol 74, 7936–7942.[CrossRef] [Google Scholar]
  4. Gamberale, R., Galmarini, C. M., Fernández Calotti, P., Jordheim, L., Sánchez Avalos, J., Dumontet, C., Geffner, J. & Giordano, M.(2003).In vitro susceptibility of CD4+ and CD8+ T cell subsets to fludarabine. Biochem Pharmacol 66, 2185–2191.[CrossRef] [Google Scholar]
  5. George, S. L., Xiang, J. & Stapleton, J. T.(2003). Clinical isolates of GB virus type C vary in their ability to persist and replicate in peripheral blood mononuclear cells. Virology 316, 191–201.[CrossRef] [Google Scholar]
  6. George, S. L., Varmaz, D. & Stapleton, J. T.(2006). GB virus C replicates in primary T and B lymphocytes. J Infect Dis 193, 451–454.[CrossRef] [Google Scholar]
  7. Handa, A. & Brown, K. E.(2000). GB virus C/hepatitis G virus replicates in human haematopoietic cells and vascular endothelial cells. J Gen Virol 81, 2461–2469. [Google Scholar]
  8. Hsieh, S. Y., Yang, P. Y., Chen, H. C. & Liaw, Y. F.(1997). Cloning and characterization of the extreme 5′-terminal sequences of the RNA genomes of GB virus C/hepatitis G virus. Proc Natl Acad Sci U S A 94, 3206–3210.[CrossRef] [Google Scholar]
  9. Laskus, T., Radkowski, M., Wang, L. F., Vargas, H. & Rakela, J.(1997). Lack of evidence for hepatitis G virus in the livers of patients co-infected with hepatitits C and G viruses. J Virol 71, 7804–7806. [Google Scholar]
  10. Laskus, T., Radkowski, M., Wang, L. F., Vargas, H. & Rakela, J.(1998). Detection of hepatitis G virus replication sites by using highly strand-specific Tth-based reverse trasncriptase PCR. J Virol 72, 3072–3075. [Google Scholar]
  11. Linnen, J., Wages, J., Zhank-Keck, Z. Y., Fry, K. E., Krawczynski, K. Z., Alter, H., Koonin, E., Gallagher, M., Alter, M. & other authors(1996). Molecular cloning and disease association of hepatitis G virus: a transfusion transmissible agent. Science 271, 505–508.[CrossRef] [Google Scholar]
  12. Mathet, V. L., Feld, M., Espinola, L., Sanchez, D. O., Ruiz, V., Mando, O., Carballal, G., Quarleri, J. F., D’Mello, F. & other authors(2003). Hepatitis B virus S gene mutants in a patient with chronic active hepatitis with circulating anti-HBs antibodies. J Med Virol 69, 18–26.[CrossRef] [Google Scholar]
  13. Mathet, V. L., López, J. L., Ruiz, V., Sanchez, D. O., Carballal, G., Campos, R. H. & Oubina, J. R.(2007). Dynamics of a hepatitis B virus e antigen minus population ascribed to genotype F during the course of a chronic infection despite the presence of anti-HBs antibodies. Virus Res 123, 72–85.[CrossRef] [Google Scholar]
  14. Mellor, J., Haydon, G., Blair, C., Livingstone, W. & Simmonds, P.(1998). Low level or absent in vivo replication of hepatitis C virus and hepatitis G virus/GB virus C in peripheral blood mononuclear cells. J Gen Virol 79, 705–714. [Google Scholar]
  15. Oubiña, J. R., Mathet, V., Feld, M., Della Latta, M. P., Ferrario, D., Verdun, R., Libonatti, O., Fernandez, J., Carballal, G. & other authors(1999). Genetic diversity of GBV-C/HGV strains among HIV infected-IVDU and blood donors from Buenos Aires. Virus Res 65, 121–129.[CrossRef] [Google Scholar]
  16. Quarleri, J. F., Mathet, V. L., Feld, M., Ferrario, D., Della Latta, M. P., Verdún, R., Sánchez, D. O. & Oubiña, J. R.(1999). GB virus C/hepatitis G virus groups and subgroups: classification by a restriction fragment length polymorphism method based on phylogenetic analysis of the 5′ untranslated region. J Clin Microbiol 37, 1340–1347. [Google Scholar]
  17. Radkowski, M., Wang, L. F., Cianciara, J., Rakela, J. & Laskus, T.(1999). Analysis of hepatitis G virus/GB virus C quasispecies and replication sites in human subjects. Biochem Biophys Res Commun 258, 296–299.[CrossRef] [Google Scholar]
  18. Ruiz, V., Espínola, L., Mathet, V. L., Perandones, C. E. & Oubiña, J. R.(2006). Design, development and evaluation of a competitive RT-PCR for quantitation of GBV-C RNA. J Virol Methods 136, 58–64.[CrossRef] [Google Scholar]
  19. Smith, D. B., Cuceanu, N., Davidson, F., Jarvis, L. M., Mokili, J. L., Hamid, S., Ldlam, C. A. & Simmonds, P.(1997). Discrimination of hepatitis G virus/GBV-C geographical variants by analysis of the 5′ non-coding region. J Gen Virol 78, 1533–1542. [Google Scholar]
  20. Stapleton, J. T., Williams, C. F. & Xiang, J.(2004). GB virus C: a beneficial infection? J Clin Microbiol 42, 3915–3919.[CrossRef] [Google Scholar]
  21. Takahashi, K., Hijikata, M., Aoyama, K., Hoshino, H., Hino, K. & Mishiro, S.(1997). Characterization of GBV-C/HGV viral genome: comparison among different isolates for a 2kb sequence that covers entire E1 and most of 5′-UTR and E2. Int Hepatol Commun 6, 253–263.[CrossRef] [Google Scholar]

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vol. , part 7, pp. 1687 - 1692

Phylogenetic tree generated by NJ constructed with GBV-C 5′ UTR sequences

Phylogenetic tree generated by NJ constructed with GBV-C E2 sequences

Alignment of E2 deduced amino acid sequences of clones isolated from plasma (PE), CD4 TL (CD4E), CD8 TL (CD8E) and BL (B) [Single PDF file](83 KB)


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