Chimeric yellow fever virus 17D (YFV-17D) and dengue virus type 2 (DENV2) carrying the surface proteins of Modoc virus (MODV), a not-known-vector (NKV) flavivirus, replicated efficiently in mammalian (Vero-B) and mosquito (C6/36) cells, whereas MODV failed to replicate in mosquito cells. Transfection of C6/36 cells with MODV RNA did not result in virus replication; however, transfection of these mosquito cells with YFV-17D or DENV2 RNA did. The inability of NKV viruses (such as MODV) to infect and replicate in arthropod cells is thus not determined by the viral envelope, but by a post-entry event.


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vol. , part 7, pp. 1693–1697

Release of viral RNA in supernatants of Vero-B and C6/36 mosquito cells infected with MODV, YFV-17D or MOD/YFV.

Release of viral RNA in supernatants of Vero-B and C6/36 mosquito cells infected with MODV, DENV2 or MOD/DENV2.

Increase in YFV RNA levels (either intracellularly or extracellularly) in Vero-B and C6/36 cells that have been transfected with YFV RNA in the absence or presence of ribavirin.

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