1887

Abstract

Chandipura virus (CHPV) is an emerging human pathogen associated with acute encephalitis and is related closely to vesicular stomatitis virus (VSV), a prototype rhabdovirus. Here, we demonstrate that the RNA polymerase L protein of CHPV exhibits a VSV-like RNA : GDP polyribonucleotidyltransferase (PRNTase) activity, which transfers the 5′-monophosphorylated (p-) viral mRNA start sequence to GDP to produce a capped RNA, and that the conserved HR motif in the CHPV L protein is essential for the PRNTase activity. Interestingly, the CHPV L protein was found to form two distinct SDS-resistant complexes with the CHPV mRNA and leader RNA start sequences; mutations in the HR motif significantly reduced the formation of the former complex (a putative covalent enzyme–pRNA intermediate in the PRNTase reaction), but not the latter complex. These results suggest that the rhabdoviral L proteins universally use the active-site HR motif for the PRNTase reaction at the step of the enzyme–pRNA intermediate formation.

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2010-05-01
2019-12-11
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References

  1. Bourhy, H., Kissi, B. & Tordo, N. ( 1993; ). Molecular diversity of the Lyssavirus genus. Virology 194, 70–81.[CrossRef]
    [Google Scholar]
  2. Chadha, M. S., Arankalle, V. A., Jadi, R. S., Joshi, M. V., Thakare, J. P., Mahadev, P. V. & Mishra, A. C. ( 2005; ). An outbreak of Chandipura virus encephalitis in the eastern districts of Gujarat state, India. Am J Trop Med Hyg 73, 566–570.
    [Google Scholar]
  3. Chang, S. H., Hefti, E., Obijeski, J. F. & Bishop, D. H. ( 1974; ). RNA transcription by the virion polymerases of five rhabdoviruses. J Virol 13, 652–661.
    [Google Scholar]
  4. Furuichi, Y. & Shatkin, A. J. ( 2000; ). Viral and cellular mRNA capping: past and prospects. Adv Virus Res 55, 135–184.
    [Google Scholar]
  5. Hoffmann, B., Schutze, H. & Mettenleiter, T. C. ( 2002; ). Determination of the complete genomic sequence and analysis of the gene products of the virus of spring viremia of carp, a fish rhabdovirus. Virus Res 84, 89–100.[CrossRef]
    [Google Scholar]
  6. Marriott, A. C. ( 2005; ). Complete genome sequences of Chandipura and Isfahan vesiculoviruses. Arch Virol 150, 671–680.[CrossRef]
    [Google Scholar]
  7. McWilliam, S. M., Kongsuwan, K., Cowley, J. A., Byrne, K. A. & Walker, P. J. ( 1997; ). Genome organization and transcription strategy in the complex GNS–L intergenic region of bovine ephemeral fever rhabdovirus. J Gen Virol 78, 1309–1317.
    [Google Scholar]
  8. Ogino, T. & Banerjee, A. K. ( 2007; ). Unconventional mechanism of mRNA capping by the RNA-dependent RNA polymerase of vesicular stomatitis virus. Mol Cell 25, 85–97.[CrossRef]
    [Google Scholar]
  9. Ogino, T. & Banerjee, A. K. ( 2008; ). Formation of guanosine(5′)tetraphospho(5′)adenosine cap structure by an unconventional mRNA capping enzyme of vesicular stomatitis virus. J Virol 82, 7729–7734.[CrossRef]
    [Google Scholar]
  10. Ogino, T., Yadav, S. P. & Banerjee, A. K. ( 2010; ). Histidine-mediated RNA transfer to GDP for unique mRNA capping by vesicular stomatitis virus RNA polymerase. Proc Natl Acad Sci U S A 107, 3463–3468.[CrossRef]
    [Google Scholar]
  11. Rao, B. L., Basu, A., Wairagkar, N. S., Gore, M. M., Arankalle, V. A., Thakare, J. P., Jadi, R. S., Rao, K. A. & Mishra, A. C. ( 2004; ). A large outbreak of acute encephalitis with high fatality rate in children in Andhra Pradesh, India, in 2003, associated with Chandipura virus. Lancet 364, 869–874.[CrossRef]
    [Google Scholar]
  12. Shuman, S. ( 2001; ). Structure, mechanism, and evolution of the mRNA capping apparatus. Prog Nucleic Acid Res Mol Biol 66, 1–40.
    [Google Scholar]
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