@article{mbs:/content/journal/jgv/10.1099/vir.0.018887-0, author = "Drexler, Jan Felix and Baumgarte, Sigrid and de Souza Luna, Luciano Kleber and Stöcker, Andreas and Almeida, Patrícia Silva and Ribeiro, Tereza Cristina Medrado and Petersen, Nadine and Herzog, Petra and Pedroso, Célia and Brites, Carlos and da Costa Ribeiro, Hugo and Gmyl, Anatoly and Drosten, Christian and Lukashev, Alexander", title = "Genomic features and evolutionary constraints in Saffold-like cardioviruses", journal= "Journal of General Virology", year = "2010", volume = "91", number = "6", pages = "1418-1427", doi = "https://doi.org/10.1099/vir.0.018887-0", url = "https://www.microbiologyresearch.org/content/journal/jgv/10.1099/vir.0.018887-0", publisher = "Microbiology Society", issn = "1465-2099", type = "Journal Article", abstract = "This study identified the complete genomic sequence of four type 2 and type 3 human Saffold-like cardioviruses (SLCVs) isolated in Germany and Brazil. The secondary structures of the SLCV internal ribosome entry sites (IRESs) were deduced based on RNA base-pairing conservation and co-variation, using an established Theiler's murine encephalomyelitis virus (TMEV) IRES structure as a reference. The SLCV IRES was highly similar to that of TMEV, but motifs critical in TMEV for binding of the polypyrimidine tract-binding protein (PTB) were disrupted. In TMEV, corresponding alterations have been associated with reduced neurovirulence in mice. In the non-structural genome region, there was evidence of multiple intertypic recombination events between different SLCV types. Between viruses of the same type, recombination also occurred in the capsid-encoding genome region. There were apparently no recombination events between mouse TMEV and human SLCV. In another genus of the family Picornaviridae, Enterovirus, natural recombination occurs strictly within species and can serve as an additional criterion for delimiting species. Accordingly, the results of this study suggest that SLCV and TMEV may represent distinct species within the genus Cardiovirus.", }