RT Journal Article SR Electronic(1) A1 Tayebi, Mourad A1 Taylor, William Alexander A1 Jones, Daryl Rhys A1 Bate, Clive A1 David, MoniqueYR 2010 T1 PrP-specific camel antibodies with the ability to immunodetect intracellular prion protein JF Journal of General Virology, VO 91 IS 8 SP 2121 OP 2131 DO https://doi.org/10.1099/vir.0.018754-0 PB Microbiology Society, SN 1465-2099, AB Although there is currently no effective treatment for prion diseases, significant advances have been made in suppressing its progress, using antibodies that block the conversion of PrPC into PrPSc. In order to be effective in treating individuals that have prion diseases, antibodies must be capable of arresting disease in its late stages. This requires the development of antibodies with higher affinity for PrPSc and systems for effective translocation of antibodies across the blood–brain barrier in order to achieve high concentrations of inhibitor at the site of protein replication. An additional advantage is the ability of these antibodies to access the cytosol of affected cells. To this end, we have generated PrP-specific antibodies (known as PrioV) by immunization of camels with murine scrapie material adsorbed to immunomagnetic beads. The PrioV antibodies display a range of specificities with some recognizing the PrP27–30 proteinase K-resistant fragment, others specific for PrPC and a number with dual binding specificity. Independent of their PrP conformation specificity, one of the PrioV antibodies (PrioV3) was shown to bind PrPC in the cytosol of neuroblastoma cells. In marked contrast, conventional anti-PrP antibodies produced in mouse against similar target antigen were unable to cross the neuronal plasma membrane and instead formed a ring around the cells. The PrioV anti-PrP antibodies could prove to be a valuable tool for the neutralization/clearance of PrPSc in intracellular compartments of affected neurons and could potentially have wider applicability for the treatment of so-called protein-misfolding diseases., UL https://www.microbiologyresearch.org/content/journal/jgv/10.1099/vir.0.018754-0