RT Journal Article SR Electronic(1) A1 Bouzalas, Ilias G. A1 Dovas, Chrysostomos I. A1 Banos, Georgios A1 Papanastasopoulou, Maria A1 Kritas, Spyridon A1 Oevermann, Anna A1 Papakostaki, Dimitra A1 Evangelia, Chatzinasiou A1 Papadopoulos, Orestis A1 Seuberlich, Torsten A1 Koptopoulos, GeorgiosYR 2010 T1 Caprine PRNP polymorphisms at codons 171, 211, 222 and 240 in a Greek herd and their association with classical scrapie JF Journal of General Virology, VO 91 IS 6 SP 1629 OP 1634 DO https://doi.org/10.1099/vir.0.017350-0 PB Microbiology Society, SN 1465-2099, AB The association between PRNP variation and scrapie incidence was investigated in a highly affected Greek goat herd. Four mutations were identified at codons 171Q/R, 211R/Q, 222Q/K and 240P/S. Lysine at codon 222 was found to be associated with the protection from natural scrapie (P=0.0111). Glutamine at codon 211 was observed in eight animals, all of them being scrapie-negative, indicating a possible protective role of this polymorphism although statistical analysis failed to support it (P=0.1074). A positive association (P=0.0457) between scrapie-affected goats and the wild-type Q171R211Q222S240 allele is presented for the first time. In addition, a novel R171RQS allele, which is identical to the A136R154R171 allele that has been associated with resistance to classical scrapie in sheep, was observed in low frequency. Resistant alleles that include K222 and Q211 are absent or rare in sheep and can provide the basis for the development of a feasible breeding programme for scrapie eradication in goats., UL https://www.microbiologyresearch.org/content/journal/jgv/10.1099/vir.0.017350-0