1887

Abstract

Of 58 enterovirus strains isolated from Japanese travellers returning from Asian countries, eight were non-serotypable with existing antisera. By sequencing a part of the VP1 region, six of these strains were typed as echovirus 9, enterovirus (EV)-73, EV-79 or EV-97. The nucleotide identity of the VP1 region of isolate T92-1499 to all enterovirus prototypes was <70 %. The VP1 sequence of isolate TN94-0349 was closely related to coxsackievirus (CV)-A9 (73.3 % nucleotide identity), but the virus could not be neutralized with a serum raised against the prototype CV-A9 strain. On the basis of complete molecular comparisons, T92-1499 and TN94-0349 were identified as EV-98 and EV-107, respectively, by the ICTV Picornavirus Study Group. Serum neutralization tests of Japanese individuals revealed a seroprevalence rate of 11 % for EV-73, and even lower seroprevalence rates, 1.0–3.8 %, were found for the other new enteroviruses, suggesting that prior circulation of these viruses in Japan was unlikely.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/vir.0.016014-0
2010-04-01
2019-11-18
Loading full text...

Full text loading...

/deliver/fulltext/jgv/91/4/1063.html?itemId=/content/journal/jgv/10.1099/vir.0.016014-0&mimeType=html&fmt=ahah

References

  1. Caro, V., Guillot, S., Delpeyroux, F. & Crainic, R. ( 2001; ). Molecular strategy for ‘serotyping’ of human enteroviruses. J Gen Virol 82, 79–91.
    [Google Scholar]
  2. Chang, K. H., Day, C., Walker, J., Hyypia, T. & Stanway, G. ( 1992; ). The nucleotide sequences of wild-type coxsackievirus A9 strains imply that an RGD motif in VP1 is functionally significant. J Gen Virol 73, 621–626.[CrossRef]
    [Google Scholar]
  3. Ishiko, H., Shimada, Y., Yonaha, M., Hashimoto, O., Hayashi, A., Sakae, K. & Takeda, N. ( 2002; ). Molecular diagnosis of human enteroviruses by phylogeny-based classification by use of the VP4 sequence. J Infect Dis 185, 744–754.[CrossRef]
    [Google Scholar]
  4. King, A. M. Q., Brown, F., Christian, P., Hovi, T., Hyypia, T., Knowles, N. J., Lemon, S. M., Minor, P. D., Palmenberg, A. C. &other authors ( 2000; ). Picornaviridae. In Virus Taxonomy: Seventh Report of the International Committee on Taxonomy of Viruses, pp. 657–678. Edited by M. H. V. van Regenmortel, C. M. Fauquet, D. H. L. Bishop, E. B. Carstens, M. K. Estes, S. M. Lemon, J. Maniloff, M. A. Mayo, D. J. McGeoch, C. R. Pringle & R. B. Wickner. San Diego: Academic Press.
  5. Lukashev, A. N., Lashkevich, V. A., Ivanova, O. E., Koroleva, G. A., Hinkkanen, A. E. & Ilonen, J. ( 2005; ). Recombination in circulating Human enterovirus B: independent evolution of structural and non-structural genome regions. J Gen Virol 86, 3281–3290.[CrossRef]
    [Google Scholar]
  6. Melnick, J. L. ( 1996; ). Enteroviruses: polioviruses, coxsackieviruses, echoviruses, and newer enteroviruses. In Fields Virology, 3rd edn, pp. 655–712. Edited by B. N. Fields, D. M. Knipe & P. M. Howley. Philadelphia: Lippincott Williams & Wilkins.
  7. Oberste, M. S., Maher, K., Kilpatrick, D. R. & Pallansh, M. A. ( 1999; ). Molecular evolution of the human enteroviruses: correlation of serotyping with VP1 sequence and application to picornavirus classification. J Virol 73, 1941–1948.
    [Google Scholar]
  8. Oberste, M. S., Maher, K., Flemister, M. R., Marchetti, G., Kilpatrick, D. R. & Pallansh, M. A. ( 2000; ). Comparison of classic and molecular approaches for the identification of untypeable enteroviruses. J Clin Microbiol 38, 1170–1174.
    [Google Scholar]
  9. Oberste, M. S., Schnurr, D., Maher, K., al-Busaidy, S. & Pallansch, M. A. ( 2001; ). Molecular identification of new picornaviruses and characterization of a proposed enterovirus 73 serotype. J Gen Virol 82, 409–416.
    [Google Scholar]
  10. Pallansch, M. A. & Roos, R. ( 2007; ). Enteroviruses: polioviruses, coxsackieviruses, echoviruses, and newer enteroviruses. In Fields Virology, 5th edn, pp. 839–893. Edited by D. M. Knipe & P. M. Howley. Philadelphia: Lippincott Williams & Wilkins.
  11. Santti, J., Harvala, H., Kinnunen, L. & Hyypia, T. ( 2000; ). Molecular epidemiology and evolution of coxsackievirus A9. J Gen Virol 81, 1361–1372.
    [Google Scholar]
  12. Yamashita, T., Sakae, K., Ishihara, Y., Isomura, S. & Utagawa, E. ( 1993; ). Prevalence of newly isolated, cytopathic small round virus (Aichi strain) in Japan. J Clin Microbiol 31, 2938–2943.
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/vir.0.016014-0
Loading
/content/journal/jgv/10.1099/vir.0.016014-0
Loading

Data & Media loading...

Supplements

Neighbour-joining phylogenetic trees for different genome fragments of the HEV-B strains studied [ PDF] (22 kb) Enterovirus strains used in Fig. S1 [ PDF] (9 kb) Prevalence rates for antibody to newly isolated enteroviruses in different age groups [ PDF] (19 kb)

PDF

Neighbour-joining phylogenetic trees for different genome fragments of the HEV-B strains studied [ PDF] (22 kb) Enterovirus strains used in Fig. S1 [ PDF] (9 kb) Prevalence rates for antibody to newly isolated enteroviruses in different age groups [ PDF] (19 kb)

PDF

Neighbour-joining phylogenetic trees for different genome fragments of the HEV-B strains studied [ PDF] (22 kb) Enterovirus strains used in Fig. S1 [ PDF] (9 kb) Prevalence rates for antibody to newly isolated enteroviruses in different age groups [ PDF] (19 kb)

PDF

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error