Given the high similarity in genome content and organization between multiple nucleopolyhedrovirus (SeMNPV) and nucleopolyhedrovirus (AgseNPV), as well as the high percentages of similarity found between their 30 core genes, the specificity of these NPVs was analysed for the respective insect hosts, and . The LD for AgseNPV in second-instar larvae was 83 occlusion bodies per larva and the LT was 8.1 days. AgseNPV was orally infectious for , but the LD was 10 000-fold higher than for SeMNPV. SeMNPV was not infectious for larvae when administered orally, but an infection was established by injection into the haemocoel. Bypassing midgut entry by intrahaemocoelic inoculation suggested that the midgut is the major barrier in larvae for infection by SeMNPV. Delayed-early genes of SeMNPV are expressed in the midgut of larvae after oral infections, indicating that the virus is able to enter midgut epithelial cells and that it proceeds through the first phases of the infection process. The possible mechanisms of resistance to SeMNPV in infections are discussed.


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vol. , part 4, pp. 898–906

Sequences of the specific primer sets used in this study [ PDF] (63 KB)


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