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Volume 91, Issue 2

Identification of a dominant endoplasmic reticulum-retention signal in yellow fever virus pre-membrane protein Free

Abstract

Yellow fever virus (YFV) encodes two envelope proteins, pre-membrane (prM) and envelope (E), that accumulate in the endoplasmic reticulum (ER). The C termini of prM and E form two antiparallel transmembrane -helices that contain ER-retention signals. To understand further the ER retention of the prME heterodimer, we characterized the subcellular localization of chimeric proteins made of a reporter protein fused to the transmembrane segments of YFV envelope proteins. We showed that at least three of the transmembrane segments of the prME heterodimer are ER-retention signals. Interestingly, increasing the length of these -helices led to the export of the chimeric proteins out of the ER. Furthermore, adding a diacidic export signal at the C terminus of the first transmembrane segment of the E protein also induced export to the cell surface. However, adding this export signal at the C terminus of the first transmembrane segment of E in the context of prME did not change the subcellular localization of the prME heterodimer, suggesting the presence of a stronger ER-retention signal outside the first transmembrane segment of E. Importantly, the diacidic export motif added to the C terminus of the first transmembrane segment of the prM protein was not sufficient to export a chimeric protein out of the ER, indicating that this sequence is a dominant ER-retention signal. Together, these data indicate that a combination of several signals of different strengths contributes to the ER retention of the YFV envelope protein heterodimer.

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2010-02-01
2024-04-25
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Identification of a dominant endoplasmic reticulum-retention signal in yellow fever virus pre-membrane protein
J. Gen. Virol. 91, 404 (2010); https://doi.org/10.1099/vir.0.015339-0
/content/journal/jgv/10.1099/vir.0.015339-0
/content/journal/jgv/10.1099/vir.0.015339-0
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