RT Journal Article SR Electronic(1) A1 Gallois, Jean-Luc A1 Charron, Carine A1 Sánchez, Flora A1 Pagny, Gaëlle A1 Houvenaghel, Marie-Christine A1 Moretti, André A1 Ponz, Fernando A1 Revers, Frédéric A1 Caranta, Carole A1 German-Retana, SylvieYR 2010 T1 Single amino acid changes in the turnip mosaic virus viral genome-linked protein (VPg) confer virulence towards Arabidopsis thaliana mutants knocked out for eukaryotic initiation factors eIF(iso)4E and eIF(iso)4G JF Journal of General Virology, VO 91 IS 1 SP 288 OP 293 DO https://doi.org/10.1099/vir.0.015321-0 PB Microbiology Society, SN 1465-2099, AB Previous resistance analyses of Arabidopsis thaliana mutants knocked out for eukaryotic translation initiation factors showed that disruption of the At-eIF(iso)4E or both the At-eIF(iso)4G1 and At-eIF(iso)4G2 genes resulted in resistance against turnip mosaic virus (TuMV). This study selected TuMV virulent variants that overcame this resistance and showed that two independent mutations in the region coding for the viral genome-linked protein (VPg) were sufficient to restore TuMV virulence in At-eIF(iso)4E and At-eIF(iso)4G1×At-eIF(iso)4G2 knockout plants. As a VPg–eIF(iso)4E interaction has been shown previously to be critical for TuMV infection, a systematic analysis of the interactions between A. thaliana eIF4Es and VPgs of virulent and avirulent TuMVs was performed. The results suggest that virulent TuMV variants may use an eIF4F-independent pathway., UL https://www.microbiologyresearch.org/content/journal/jgv/10.1099/vir.0.015321-0