1887

Abstract

Previous resistance analyses of mutants knocked out for eukaryotic translation initiation factors showed that disruption of the or both the and genes resulted in resistance against turnip mosaic virus (TuMV). This study selected TuMV virulent variants that overcame this resistance and showed that two independent mutations in the region coding for the viral genome-linked protein (VPg) were sufficient to restore TuMV virulence in - and × knockout plants. As a VPg–eIF(iso)4E interaction has been shown previously to be critical for TuMV infection, a systematic analysis of the interactions between eIF4Es and VPgs of virulent and avirulent TuMVs was performed. The results suggest that virulent TuMV variants may use an eIF4F-independent pathway.

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2010-01-01
2019-11-21
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vol. , part 1, pp. 288 - 293

Oligonucleotides used for PCR fusion-based mutagenesis experiments

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