1887

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Volume 91, Issue 2

Parvovirus PARV4 visualization and detection Free

Abstract

The parvovirus PARV4 is the most recently described member of the family that has a human host. To investigate the prevalence of PARV4 in blood, a quantitative TaqMan PCR was developed and plasma, sera or whole blood from a variety of population groups were examined. Eight samples were positive for PARV4, one at high copy number. The high-titre-positive plasma had an approximate viral load of 5×10 genome equivalents ml. Two human sera, identified as PARV4 antibody-positive by indirect immunofluorescence, were used in immune electron microscopy to try to visualize native PARV4 within the high-titre human plasma. PARV4 particles were observed using one of these two sera. To our knowledge, this is the first time that native PARV4 has been visualized.

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  • Accepted:
  • Published Online:
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2010-02-01
2023-09-27
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Parvovirus PARV4 visualization and detection
J. Gen. Virol. 91, 541 (2010); https://doi.org/10.1099/vir.0.014852-0
/content/journal/jgv/10.1099/vir.0.014852-0
/content/journal/jgv/10.1099/vir.0.014852-0
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