1887

Abstract

For a better understanding of the functionality and pathogenicity of beet soil-borne virus (BSBV), full-length cDNA clones have been constructed for the three genomic RNAs. With the aim of assessing their effectiveness and relative contribution to the virus housekeeping functions, transcripts were inoculated on and leaves using five genome combinations. Both RNAs-1 (putative replicase) and -3 (putative movement proteins) proved to be essential for virus replication and symptom production on , whereas RNA-2 (putative coat protein, CP, and a read-through domain, RT) was not. No symptoms were recorded on , but viral RNAs were detected. In both host plants, the 19 kDa CP was detected by Western blotting as well as a 115 kDa protein corresponding to the CP–RT.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/vir.0.014548-0
2009-12-01
2019-11-22
Loading full text...

Full text loading...

/deliver/fulltext/jgv/90/12/3051.html?itemId=/content/journal/jgv/10.1099/vir.0.014548-0&mimeType=html&fmt=ahah

References

  1. Adams, M. J., Antoniw, J. F. & Mullins, J. G. L. ( 2001; ). Plant virus transmission by plasmodiophorid fungi is associated with distinctive transmembrane regions of virus-encoded proteins. Arch Virol 146, 1139–1153.[CrossRef]
    [Google Scholar]
  2. Akamatsu, N., Takeda, A., Kishimoto, M., Kaido, M., Okuno, T. & Mise, K. ( 2007; ). Phosphorylation and interaction of the movement and coat proteins of Brome mosaic virus in infected barley protoplasts. Arch Virol 152, 2087–2093.[CrossRef]
    [Google Scholar]
  3. Bouzoubaa, S., Ziegler, V., Beck, D., Guilley, H., Richards, K. & Jonard, G. ( 1986; ). Nucleotide sequence of beet necrotic yellow vein virus RNA-2. J Gen Virol 67, 1689–1700.[CrossRef]
    [Google Scholar]
  4. Bouzoubaa, S., Quillet, L., Guilley, H., Jonard, G. & Richards, K. ( 1987; ). Nucleotide sequence of beet necrotic yellow vein virus RNA-1. J Gen Virol 68, 615–626.[CrossRef]
    [Google Scholar]
  5. Canova, A. ( 1959; ). On the pathology of sugar beet. Inf Fitopatol 9, 390–396.
    [Google Scholar]
  6. Crutzen, F., Kreit, M. & Bragard, C. ( 2009; ). The Beet virus Q coat protein readthrough domain is longer than previously reported, with two transmembrane domains. J Gen Virol 90, 754–758.[CrossRef]
    [Google Scholar]
  7. Diao, A., Chen, J., Gitton, F., Antoniw, J. F., Mullins, J., Hall, A. M. & Adams, M. J. ( 1999; ). Sequences of European wheat mosaic virus and Oat golden stripe virus and genome analysis of the genus Furovirus. Virology 261, 331–339.[CrossRef]
    [Google Scholar]
  8. Duggal, R., Lahser, F. C. & Hall, T. C. ( 1994; ). cis-Acting sequences in the replication of plant viruses with plus-sense RNA genomes. Annu Rev Phytopathol 32, 287–309.[CrossRef]
    [Google Scholar]
  9. Dunoyer, P., Pfeffer, S., Fritsch, C., Hemmer, O., Voinnet, O. & Richards, K. E. ( 2002; ). Identification, subcellular localization and some properties of a cysteine-rich suppressor of gene silencing encoded by Peanut clump virus. Plant J 29, 555–567.[CrossRef]
    [Google Scholar]
  10. Hafrén, A. & Mäkinen, K. ( 2008; ). Purification of viral genome-linked protein VPg from Potato virus A-infected plants reveals several post-translationally modified forms of the protein. J Gen Virol 89, 1509–1518.[CrossRef]
    [Google Scholar]
  11. Hahn, P. & Shepherd, R. J. ( 1980; ). Phosphorylated proteins in Cauliflower mosaic virus. Virology 107, 295–297.[CrossRef]
    [Google Scholar]
  12. Henry, C. M., Jones, R. A. C. & Coutts, R. H. A. ( 1986; ). Occurrence of a soil-borne virus of sugar beet in England. Plant Pathol 35, 585–591.[CrossRef]
    [Google Scholar]
  13. Hu, C.-C. & Ghabrial, S. A. ( 1995; ). The conserved, hydrophilic and arginine-rich N-terminal domain of cucumovirus coat proteins contributes to their anomalous electrophoretic mobilities in sodium dodecylsulfate-polyacrylamide gels. J Virol Methods 55, 367–379.[CrossRef]
    [Google Scholar]
  14. Hull, R. ( 2009; ). How do plant virus work? Virus–plant interactions: molecular level. In Comparative Plant Virology, 2nd edn, pp. 191–206. London: Academic Press.
  15. Ivanović, M., Macfarlane, I. & Woods, R. D. ( 1983; ). Viruses of sugar beet associated with Polymyxa betae. In Annual Report of Rothamsted Experimental Station for 1982, pp. 189–190. Harpenden, UK: IARC Rothamsted.
  16. Jupin, I., Richards, K., Jonard, G., Guilley, H. & Pleij, C. W. ( 1990; ). Mapping sequences required for productive replication of Beet necrotic yellow vein virus RNA 3. Virology 178, 273–280.[CrossRef]
    [Google Scholar]
  17. Kaufmann, A., Koenig, R. & Rohloff, H. ( 1993; ). Influence of beet soil-borne virus on mechanically inoculated sugar beet. Plant Pathol 42, 413–417.[CrossRef]
    [Google Scholar]
  18. Koenig, R. & Loss, S. ( 1997; ). Beet soil-borne virus RNA 1: genetic analysis enabled by a starting sequence generated with primers to highly conserved helicase-encoding domains. J Gen Virol 78, 3161–3165.
    [Google Scholar]
  19. Koenig, R., Beier, C., Commandeur, U., Lüth, U., Kaufmann, A. & Lüddecke, P. ( 1996; ). Beet soil-borne virus RNA 3 – a further example of the heterogeneity of the gene content of furovirus genomes and of triple gene block-carrying RNAs. Virology 216, 202–207.[CrossRef]
    [Google Scholar]
  20. Koenig, R., Commandeur, U., Loss, S., Beier, C., Kaufmann, A. & Lesemann, D.-E. ( 1997; ). Beet soil-borne virus RNA 2: similarities and dissimilarities to the coat protein gene-carrying RNAs of other furoviruses. J Gen Virol 78, 469–477.
    [Google Scholar]
  21. Koenig, R., Pleij, C. W. A. & Büttner, G. ( 2000; ). Structure and variability of the 3′ end of RNA 3 of Beet soil-borne pomovirus – a virus with uncertain pathogenic effects. Arch Virol 145, 1173–1181.[CrossRef]
    [Google Scholar]
  22. Lauber, E., Guilley, H., Tamada, T., Richards, K. E. & Jonard, G. ( 1998; ). Vascular movement of beet necrotic yellow vein virus in Beta macrocarpa is probably dependent on an RNA 3 sequence domain rather than a gene product. J Gen Virol 79, 385–393.
    [Google Scholar]
  23. Lindsten, K. ( 1993; ). Rhizomania – are both Beet necrotic yellow vein virus (BNYVV) and Beet soil-borne virus (BSBV) involved? In Proceedings of the Second Symposium of the International Working Group on Plant Viruses with Fungal Vectors, 2nd edn, pp. 67–70. Edited by C. Hiruki, American Society of Sugar Beet Technologists, Denver.
  24. McGeachy, K. D. & Barker, H. ( 2000; ). Potato mop-top virus RNA can move long distance in the absence of coat protein: evidence from resistant, transgenic plants. Mol Plant Microbe Interact 13, 125–128.[CrossRef]
    [Google Scholar]
  25. McGrann, G. R., Grimmer, M. K., Mutasa-Göttgens, E. S. & Stevens, M. ( 2009; ). Progress towards the understanding and control of sugar beet rhizomania disease. Mol Plant Pathol 10, 129–141.[CrossRef]
    [Google Scholar]
  26. Meunier, A., Schmit, J.-F., Stas, A., Kutluk, N. & Bragard, C. ( 2003; ). Multiplex reverse transcription-PCR for simultaneous detection of Beet necrotic yellow vein virus, Beet soilborne virus and Beet virus Q and their vector Polymyxa betae KESKIN on sugar beet. Appl Environ Microbiol 69, 2356–2360.[CrossRef]
    [Google Scholar]
  27. Prillwitz, H. & Schlösser, E. ( 1992; ).. Beet soil-borne virus: occurrence, symptoms and effect on plant development. In 44th International Symposium on Crop Protection. Ghent, Belgium, May 1992, pp. 295–302.
  28. Rahim, M. D., Andika, I. B., Han, C., Kondo, H. & Tamada, T. ( 2007; ). RNA4-encoded p31 of beet necrotic yellow vein virus is involved in efficient vector transmission, symptom severity and silencing suppression in roots. J Gen Virol 88, 1611–1619.[CrossRef]
    [Google Scholar]
  29. Rath, A., Glibowicka, M., Nadeau, V. G., Chen, G. & Deber, C. M. ( 2009; ). Detergent binding explains anomalous SDS-PAGE migration of membrane proteins. Proc Natl Acad Sci U S A 106, 1760–1765.[CrossRef]
    [Google Scholar]
  30. Rush, C. M. & Heidel, G. B. ( 1995; ). Furovirus diseases of sugar beets in the United States. Plant Dis 79, 868–875.[CrossRef]
    [Google Scholar]
  31. Savenkov, E. I., Germundsson, A., Zamyatnin, A. A., Jr, Sandgren, M. & Valkonen, J. P. ( 2003; ). Potato mop-top virus: the coat protein-encoding RNA and the gene for cysteine-rich protein are dispensable for systemic virus movement in Nicotiana benthamiana. J Gen Virol 84, 1001–1005.[CrossRef]
    [Google Scholar]
  32. Seddas, P. & Boissinot, S. ( 2006; ). Glycosylation of Beet western yellows virus proteins is implicated in the aphid transmission of the virus. Arch Virol 151, 967–984.[CrossRef]
    [Google Scholar]
  33. Siegel, A., Zaitlin, M. & Sehgal, O. P. ( 1962; ). The isolation of defective Tobacco mosaic virus strains. Proc Natl Acad Sci USA 48, 1845–1851.[CrossRef]
    [Google Scholar]
  34. Tamada, T. & Baba, T. ( 1973; ). Beet necrotic yellow vein virus from rhizomania-affected sugar beet in Japan. Ann Phytopathol Soc Jpn 39, 325–332.[CrossRef]
    [Google Scholar]
  35. Tamada, T., Shirako, Y., Abe, H., Saito, M., Kiguchi, T. & Harada, T. ( 1989; ). Production and pathogenicity of isolates of Beet necrotic yellow vein virus with different numbers of RNA components. J Gen Virol 70, 3399–3409.[CrossRef]
    [Google Scholar]
  36. Zayakina, O., Arkhipenko, M., Kozlovsky, S., Nikitin, N., Smirnov, A., Susi, P., Rodionova, N., Karpova, O. & Atabekov, J. ( 2008; ). Mutagenic analysis of potato virus X movement protein (TGBp1) and the coat protein (CP): in vitro TGBp1-CP binding and viral RNA translation activation. Mol Plant Pathol 9, 37–44.
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/vir.0.014548-0
Loading
/content/journal/jgv/10.1099/vir.0.014548-0
Loading

Data & Media loading...

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error