1887

Abstract

Some pigeon paramyxovirus type 1 (PPMV-1) strains exhibit low virulence in chickens, despite their fusion (F) protein's multi-basic cleavage site. To elucidate the molecular basis of the low pathogenicity of these strains, we constructed an infectious full-length cDNA clone of PPMV-1 strain AV324. This strain is non-virulent for chickens, although its F protein contains the typical virulence motif RRKKRF. By using reverse genetics, we exchanged the F genes of AV324 and a virulent Newcastle disease virus (NDV) strain (Herts) and evaluated the recovered chimeric viruses for their pathogenicity in 1-day-old chickens and in embryonated eggs. Our results show that the F protein of AV324, and probably those of similar PPMV-1 strains, are functionally not different from those of virulent NDV strains and that the difference in pathogenicity must be determined by other factors.

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2009-11-01
2020-10-29
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References

  1. Alexander D. J., Gough R. E. 2003; Newcastle disease, other avian paramyxoviruses and pneumovirus infections. In Diseases of Poultry , 11th edn. pp 63–92Edited by Saif Y. M., Barnes H. J., Fadly A. M., Glisson J. R., McDougald L. R., Swayne D. E. Ames, IA: Iowa State University Press;
    [Google Scholar]
  2. Antin P. B., Ordahl C. P. 1991; Isolation and characterization of an avian myogenic cell line. Dev Biol 143:111–121 [CrossRef]
    [Google Scholar]
  3. Britton P., Green P., Kottier S., Mawditt K. L., Penzes Z., Cavanagh D., Skinner M. A. 1996; Expression of bacteriophage T7 RNA polymerase in avian and mammalian cells by a recombinant fowlpox virus. J Gen Virol 77:963–967 [CrossRef]
    [Google Scholar]
  4. Collins M. S., Strong I., Alexander D. J. 1996; Pathogenicity and phylogenetic evaluation of the variant Newcastle disease viruses termed “pigeon PMV-1 viruses” based on the nucleotide sequence of the fusion protein gene. Arch Virol 141:635–647 [CrossRef]
    [Google Scholar]
  5. Council of the European Communities 1992; Council directive 92/66/EEC of; 14 July 1992; introducing community measures for the control of Newcastle disease. Offic J Eur Communities L260:1–20
    [Google Scholar]
  6. de Leeuw O., Peeters B. 1999; Complete nucleotide sequence of Newcastle disease virus: evidence for the existence of a new genus within the subfamily Paramyxovirinae . J Gen Virol 80:131–136
    [Google Scholar]
  7. de Leeuw O. S., Koch G., Hartog L., Ravenshorst N., Peeters B. P. 2005; Virulence of Newcastle disease virus is determined by the cleavage site of the fusion protein and by both the stem region and globular head of the haemagglutinin–neuraminidase protein. J Gen Virol 86:1759–1769 [CrossRef]
    [Google Scholar]
  8. Fuller C. M., Collins M. S., Easton A. J., Alexander D. J. 2007; Partial characterisation of five cloned viruses differing in pathogenicity, obtained from a single isolate of pigeon paramyxovirus type 1 (PPMV-1) following passage in fowls' eggs. Arch Virol 152:1575–1582 [CrossRef]
    [Google Scholar]
  9. Garten W., Berk W., Nagai Y., Rott R., Klenk H. D. 1980; Mutational changes of the protease susceptibility of glycoprotein F of Newcastle disease virus: effects on pathogenicity. J Gen Virol 50:135–147 [CrossRef]
    [Google Scholar]
  10. Hanson R. P. 1975; Newcastle disease. In Isolation and Identification of Avian Pathogens . pp 160–173Edited by Hitchner S. B., Domermuth C. H., Purchase H. G., Williams J. E. Kennett Square, PA: American Association of Avian Pathologists;
  11. Mayo M. A. 2002; A summary of taxonomic changes recently approved by ICTV. Arch Virol 147:1655–1663 [CrossRef]
    [Google Scholar]
  12. Meulemans G., van den Berg T. P., Decaesstecker M., Boschmans M. 2002; Evolution of pigeon Newcastle disease virus strains. Avian Pathol 31:515–519 [CrossRef]
    [Google Scholar]
  13. Nagai Y., Klenk H. D., Rott R. 1976; Proteolytic cleavage of the viral glycoproteins and its significance for the virulence of Newcastle disease virus. Virology 72:494–508 [CrossRef]
    [Google Scholar]
  14. Ogasawara T., Gotoh B., Suzuki H., Asaka J., Shimokata K., Rott R., Nagai Y. 1992; Expression of factor X and its significance for the determination of paramyxovirus tropism in the chick embryo. EMBO J 11:467–472
    [Google Scholar]
  15. Pedersen J. C., Senne D. A., Woolcock P. R., Kinde H., King D. J., Wise M. G., Panigrahy B., Seal B. S. 2004; Phylogenetic relationships among virulent Newcastle disease virus isolates from the 2002–2003 outbreak in California and other recent outbreaks in North America. J Clin Microbiol 42:2329–2334 [CrossRef]
    [Google Scholar]
  16. Peeters B. P., de Leeuw O. S., Koch G., Gielkens A. L. 1999; Rescue of Newcastle disease virus from cloned cDNA: evidence that cleavability of the fusion protein is a major determinant for virulence. J Virol 73:5001–5009
    [Google Scholar]
  17. Reed L. J., Muench H. A. 1938; A simple method of estimating fifty percent endpoints. Am J Hyg 27:493–497
    [Google Scholar]
  18. Rott R., Klenk H. D. 1988; Molecular basis of infectivity and pathogenicity of Newcastle disease virus. In Newcastle Disease pp 98–112Edited by Alexander D. J. Boston: Kluwer Academic;
    [Google Scholar]
  19. Terregino C., Cattoli G., Grossele B., Bertoli E., Tisato E., Capua I. 2003; Characterization of Newcastle disease virus isolates obtained from Eurasian collared doves ( Streptopelia decaocto ) in Italy. Avian Pathol 32:63–68 [CrossRef]
    [Google Scholar]
  20. Ujvari D. 2006; Complete nucleotide sequence of IT-227/82, an avian paramyxovirus type-1 strain of pigeons ( Columba livia ). Virus Genes 32:49–57 [CrossRef]
    [Google Scholar]
  21. Wensvoort G., Terpstra C., Boonstra J., Bloemraad M., Van Zaane D. 1986; Production of monoclonal antibodies against swine fever virus and their use in laboratory diagnosis. Vet Microbiol 12:101–108 [CrossRef]
    [Google Scholar]
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