The regions at the left and right ends of fowl adenovirus (FAdV) genomes are not well-characterized in comparison to those of human adenoviruses. Using a series of deletion mutants, we analysed a 2.4 kb region near the left end of the FAdV-9 genome (nt 400–2782) that contains packaging-signal motifs VI and VII and open reading frames (ORFs) 0, 1, 1A, 1B, 1C and 2. Viable viruses with specific deletions in this region had wild-type characteristics , as measured by cytopathic effect, plaque morphology, virus titres and growth kinetics. However, one mutant (FAdV-9Δ4), which lacked these ORFs and retained the packaging motifs, did not replicate at wild-type levels , as judged in infected eggs by virus titres in allantoic fluid and in infected chickens by antibody responses, virus titres in faeces and virus genome copy numbers in tissues. These findings indicate that some of the ORFs in this region, although dispensable , are important for replication of FAdV-9.


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Generation of deletions [ PDF] (60 KB)


List of primers and their positions within the FAdV-9 genome [ PDF] (96 KB)


Generation of FAdV-9 genomes with deletions [ PDF] (336 KB)


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