@article{mbs:/content/journal/jgv/10.1099/vir.0.013334-0, author = "Weng, Qingbei and Yang, Kai and Xiao, Wei and Yuan, Meijin and Zhang, Wenjun and Pang, Yi", title = "Establishment of an insect cell clone that harbours a partial baculoviral genome and is resistant to homologous virus infection", journal= "Journal of General Virology", year = "2009", volume = "90", number = "12", pages = "2871-2876", doi = "https://doi.org/10.1099/vir.0.013334-0", url = "https://www.microbiologyresearch.org/content/journal/jgv/10.1099/vir.0.013334-0", publisher = "Microbiology Society", issn = "1465-2099", type = "Journal Article", abstract = "After serially undiluted passage of Spodoptera exigua multiple nucleopolyhedrovirus (SeMNPV), persistently infected Se301 cells were established. A cell strain, in which no polyhedra or viral particles were observed, was cloned and designated P8-Se301-C1. The P8-Se301-C1 cells are morphologically similar to but grow slower than Se301 cells and they can homologously interfere with SeMNPV. PCR analysis showed that SeMNPV ie-0 and polyhedrin genes were present but DNA polymerase and orf67 genes were absent in P8-Se301-C1, suggesting that the cells harbour incomplete SeMNPV genomes. Dot-blot analysis demonstrated that 0.32±0.16 ng SeMNPV DNA was present in 1.25×105 P8-Se301-C1 cells. A quantitative real-time PCR assay showed that there were 13.2±4.3 copies of the SeMNPV polyhedrin gene in each cell. Nested RT-PCR demonstrated the presence of SeMNPV polyhedrin transcripts in P8-Se301-C1 cells. The fact that P8-Se301-C1 cells carry low levels of partial viral genome but do not produce viral progeny suggests a latent-like viral infection in the cells.", }