Transduction of vertebrate cells with multiple nucleopolyhedrovirus F protein-pseudotyped -null multiple nucleopolyhedrovirus Free

Abstract

Budded virions of the baculovirus multiple nucleopolyhedrovirus (AcMNPV) can enter a variety of non-host cells. The capacity of GP64, AcMNPV's endogenous envelope fusion protein, and F, the fusion protein from a baculovirus, to mediate baculovirus entry into vertebrate cells was examined by comparing the transduction efficiencies of engineered AcMNPV variants with either of the two envelope proteins into 17 vertebrate cell lines. At an m.o.i. of 500, GP64-expressing viruses transduced all cell lines with varying efficiencies. Transduction efficiencies of F-pseudotyped -null AcMNPV into all cell lines were lower than those of GP64-expressing viruses, and were undetectable in seven cell lines. At an m.o.i. of 50, transduction of all mammalian cell lines transducible by the F-pseudotyped -null AcMNPV at an m.o.i. of 500 was no longer detectable. An amplifiable F-pseudotyped -null AcMNPV vector with greatly reduced tropism for vertebrate cells may have applications in engineering AcMNPV for targeted transduction.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/vir.0.012138-0
2009-09-01
2024-03-28
Loading full text...

Full text loading...

/deliver/fulltext/jgv/90/9/2282.html?itemId=/content/journal/jgv/10.1099/vir.0.012138-0&mimeType=html&fmt=ahah

References

  1. Airenne, K. J., Hiltunen, M. O., Turunen, M. P., Turunen, A. M., Laitinen, O. H., Kulomaa, M. S. & Ylä-Herttuala, S.(2000). Baculovirus-mediated periadventitial gene transfer to rabbit carotid artery. Gene Ther 7, 1499–1504.[CrossRef] [Google Scholar]
  2. Barsoum, J., Brown, R., McKee, M. & Boyce, F. M.(1997). Efficient transduction of mammalian cells by a recombinant baculovirus having the vesicular stomatitis virus G glycoprotein. Hum Gene Ther 8, 2011–2018.[CrossRef] [Google Scholar]
  3. Blissard, G. W. & Wenz, J. R.(1992). Baculovirus gp64 envelope glycoprotein is sufficient to mediate pH-dependent membrane fusion. J Virol 66, 6829–6835. [Google Scholar]
  4. Boyce, F. M. & Bucher, N. L. R.(1996). Baculovirus-mediated gene transfer into mammalian cells. Proc Natl Acad Sci U S A 93, 2348–2352.[CrossRef] [Google Scholar]
  5. Chuang, C. K., Sung, L. Y., Hwang, S. M., Lo, W. H., Chen, H. C. & Hu, Y. C.(2007). Baculovirus as a new gene delivery vector for stem cell engineering and bone tissue engineering. Gene Ther 14, 1417–1424.[CrossRef] [Google Scholar]
  6. Condreay, J. P., Witherspoon, S. M., Clay, W. C. & Kost, T. A.(1999). Transient and stable gene expression in mammalian cells transduced with a recombinant baculovirus vector. Proc Natl Acad Sci U S A 96, 127–132.[CrossRef] [Google Scholar]
  7. Hefferon, K. L., Oomens, A. G. P., Monsma, S. A., Finnerty, C. M. & Blissard, G. W.(1999). Host cell receptor binding by baculovirus GP64 and kinetics of virion entry. Virology 258, 455–468.[CrossRef] [Google Scholar]
  8. Ho, Y. C., Chung, Y. C., Hwang, S. M., Wang, K. C. & Hu, Y. C.(2005). Transgene expression and differentiation of baculovirus-transduced human mesenchymal stem cells. J Gene Med 7, 860–868.[CrossRef] [Google Scholar]
  9. Hofmann, C., Sandig, V., Jennings, G., Rudolph, M., Schlag, P. & Strauss, M.(1995). Efficient gene transfer into human hepatocytes by baculovirus vectors. Proc Natl Acad Sci U S A 92, 10099–10103.[CrossRef] [Google Scholar]
  10. Hsu, C.-S., Ho, Y.-C., Wang, K.-C. & Hu, Y.-C.(2004). Investigation of optimal transduction conditions for baculovirus-mediated gene delivery into mammalian cells. Biotechnol Bioeng 88, 42–51.[CrossRef] [Google Scholar]
  11. Hu, Y.-C.(2006). Baculovirus vectors for gene therapy. Adv Virus Res 68, 287–320. [Google Scholar]
  12. Kitagawa, Y., Tani, H., Limn, C. K., Matsunaga, T. M., Moriishi, K. & Matsuura, Y.(2005). Ligand-directed gene targeting to mammalian cells by pseudotype baculoviruses. J Virol 79, 3639–3652.[CrossRef] [Google Scholar]
  13. Kost, T. A., Condreay, J. P. & Jarvis, D. L.(2005). Baculovirus as versatile vectors for protein expression in insect and mammalian cells. Nat Biotechnol 23, 567–575.[CrossRef] [Google Scholar]
  14. Leisy, D. J., Lewis, T. D., Jo-Ann, C. & Rohrmann, G. F.(2003). Transduction of cultured fish cells with recombinant baculoviruses. J Gen Virol 84, 1173–1178.[CrossRef] [Google Scholar]
  15. Liang, C. Y., Wang, H. Z., Li, T. X., Hu, Z. H. & Chen, X. W.(2004). High efficiency gene transfer into mammalian kidney cells using baculovirus vectors. Arch Virol 149, 51–60. [Google Scholar]
  16. Liang, C., Song, J. & Chen, X.(2005). The GP64 protein of Autographa californica multiple nucleopolyhedrovirus rescues Helicoverpa armigera nucleopolyhedrovirus transduction in mammalian cells. J Gen Virol 86, 1629–1635.[CrossRef] [Google Scholar]
  17. Long, G., Pan, X., Kormelink, R. & Vlak, J. M.(2006). Functional entry of baculovirus into insect and mammalian cells is dependent on clathrin-mediated endocytosis. J Virol 80, 8830–8833.[CrossRef] [Google Scholar]
  18. Lu, L., Yu, L. & Kwang, J.(2007). Baculovirus surface-displayed hemagglutinin of H5N1 influenza virus sustains its authentic cleavage, hemagglutination activity, and antigenicity. Biochem Biophys Res Commun 358, 404–409.[CrossRef] [Google Scholar]
  19. Lung, O., Westenberg, M., Vlak, J. M., Zuidema, D. & Blissard, G. W.(2002). Pseudotyping Autographa californica multicapsid nucleopolyhedrovirus (AcMNPV): F proteins from group II NPVs are functionally analogous to AcMNPV GP64. J Virol 76, 5729–5736.[CrossRef] [Google Scholar]
  20. Mäkelä, A. R., Matilainen, H., White, D. J., Ruoslahti, E. & Oker-Blom, C.(2006). Enhanced baculovirus-mediated transduction of human cancer cells by tumor-homing peptides. J Virol 80, 6603–6611.[CrossRef] [Google Scholar]
  21. Mäkelä, A. R., Enbäck, J., Vihinen-Ranta, M., Laakkonen, P. & Oker-Blom, C.(2008). Tumor targeting of baculovirus displaying a lymphatic homing peptide. J Gene Med 10, 1019–1031.[CrossRef] [Google Scholar]
  22. Mangor, J. T., Monsma, S. A., Johnson, M. C. & Blissard, G. W.(2001). A GP64-null baculovirus pseudotyped with vesicular stomatitis virus G protein. J Virol 75, 2544–2556.[CrossRef] [Google Scholar]
  23. Matilainen, H., Rinne, J., Gilbert, L., Marjomäki, V., Reunanen, H. & Oker-Blom, C.(2005). Baculovirus entry into human hepatoma cells. J Virol 79, 15452–15459.[CrossRef] [Google Scholar]
  24. Oomens, A. G. P. & Blissard, G. W.(1999). Requirement for GP64 to drive efficient budding of Autographa californica multicapsid nucleopolyhedrovirus. Virology 254, 297–314.[CrossRef] [Google Scholar]
  25. Pearson, M. N. & Rohrmann, G. F.(2002). Transfer, incorporation, and substitution of envelope fusion proteins among members of the Baculoviridae, Orthomyxoviridae, and Metaviridae (insect retrovirus) families. J Virol 76, 5301–5304.[CrossRef] [Google Scholar]
  26. Ping, W., Ge, J., Li, S., Zhou, H., Wang, K., Feng, Y. & Lou, Z.(2006). Baculovirus-mediated gene expression in chicken primary cells. Avian Dis 50, 59–63.[CrossRef] [Google Scholar]
  27. Song, J., Liang, C. & Chen, X.(2006). Transduction of avian cells with recombinant baculovirus. J Virol Methods 135, 157–162.[CrossRef] [Google Scholar]
  28. Tani, H., Nishijima, M., Ushijima, H., Miyamura, T. & Matsuura, Y.(2001). Characterization of cell-surface determinants important for baculovirus infection. Virology 279, 343–353.[CrossRef] [Google Scholar]
  29. van Oers, M. M.(2006). Vaccines for viral and parasitic diseases produced with baculovirus vectors. Adv Virus Res 68, 193–253. [Google Scholar]
  30. Volkman, L. E. & Goldsmith, P. A.(1983).In vitro survey of Autographa californica nuclear polyhedrosis virus interaction with nontarget vertebrate host cells. Appl Environ Microbiol 45, 1085–1093. [Google Scholar]
  31. Wang, C. Y., Li, F., Yang, Y., Guo, H. Y., Wu, C. X. & Wang, S.(2006). Recombinant baculovirus containing the diptheria toxin A gene for malignant glioma therapy. Cancer Res 66, 5798–5806.[CrossRef] [Google Scholar]
  32. Westenberg, M., Wang, H., IJkel, W. F. J., Goldbach, R. W., Vlak, J. M. & Zuidema, D.(2002). Furin is involved in baculovirus envelope fusion protein activation. J Virol 76, 178–184.[CrossRef] [Google Scholar]
  33. Westenberg, M., Uijtdewilligen, P. & Vlak, J. M.(2007). Baculovirus envelope fusion proteins F and GP64 exploit distinct receptors to gain entry into cultured insect cells. J Gen Virol 88, 3302–3306.[CrossRef] [Google Scholar]
  34. Yin, F., Wang, M., Tan, Y., Deng, F., Vlak, J. M., Hu, Z. & Wang, H.(2008). A functional F analogue of Autographa californica nucleopolyhedrovirus GP64 from the Agrotis segetum granulovirus. J Virol 82, 8922–8926.[CrossRef] [Google Scholar]
  35. Zhou, J. & Blissard, G. W.(2008). Display of heterologous proteins on gp64null baculovirus virions and enhanced budding mediated by a vesicular stomatitis virus G-stem construct. J Virol 82, 1368–1377.[CrossRef] [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/vir.0.012138-0
Loading
/content/journal/jgv/10.1099/vir.0.012138-0
Loading

Data & Media loading...

Most cited Most Cited RSS feed