1887

Abstract

Nucleopolyhedroviruses (NPVs), family , are insect-specific viruses with the potential to control insect pests in agriculture and forestry. NPVs are occluded in polyhedral occlusion bodies. Polyhedra protect virions from inactivation in the environment as well as assisting virions in horizontal transmission in the insect population. The process of virion occlusion in the polyhedra is undefined and the genes that regulate the virion occlusion process have not been well investigated yet. An multiple nucleopolyhedrovirus (AcMNPV) mutant (AcDef) that has a 2136 bp DNA deletion, including , and genes, has been isolated. No virions were detected in the polyhedra of AcDef. Restoration of all the missing sequences into AcDef led to proper virion occlusion. Individual gene deletion of either or could not abolish virion occlusion in the polyhedra of AcMNPV, but deletion reduced virion occlusion efficiency more than threefold compared with the wild-type AcMNPV. Previous studies by other research groups on deletion of AcMNPV gene suggested that is a infectivity factor, and deletion of the gene did not eliminate virion occlusion. Collectively, the three genes (, and may act in concert to regulate the virion occlusion process. Therefore, and are all required for proper virion occlusion in the polyhedra of AcMNPV.

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2009-07-01
2019-10-14
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