1887

Abstract

It has been shown previously that suppressive virus-specific FoxP3 regulatory CD8 T cells can be expanded from human peripheral blood mononuclear cells after antigen-specific stimulation. This study extended this finding by analysing the mechanisms of virus-specific FoxP3 regulatory CD8 T-cell generation during peptide-specific expansion . It was shown that hepatitis C virus (HCV)-, influenza virus (FLU)-, Epstein–Barr virus (EBV)- and cytomegalovirus (HCMV)-specific FoxP3 regulatory CD8 T cells could be expanded differentially from the blood of chronically HCV-infected patients following peptide-specific stimulation. The different ability of virus-specific CD8 T-cell populations to express FoxP3 after continuous antigen stimulation correlated significantly with the differentiation status. Indeed, CD27 CD28 CD57 HCV-, FLU- and EBV-specific CD8 T cells displayed a significantly higher ability to give rise to FoxP3 regulatory CD8 T cells compared with CD27 CD28 CD57 HCMV-specific CD8 T cells. Similar T-cell receptor expression patterns of FoxP3 versus FoxP3 CD8 T cells of the same antigen specificity indicated that both cell populations were probably expanded from the same virus-specific CD8 T-cell precursor. In addition, no specific antigen-presenting cell populations were required for the generation of FoxP3 CD8 T cells, as CD8-selected virus-specific FoxP3 CD8 T cells could be expanded by peptide presentation in the absence of antigen-presenting cells. Taken together, these results suggest that the ability to expand FoxP3 regulatory CD8 T cells from virus-specific CD8 T cells differs among distinct virus-specific CD8 T-cell populations depending on the differentiation status.

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2009-07-01
2020-04-02
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