1887

Journal of General Virology header logo

Volume 90, Issue 7

A novel hepatitis B virus subgenotype, D7, in Tunisian blood donors Free

Abstract

Tunisia is a medium-level epidemic country for hepatitis B virus (HBV). This study characterizes, for the first time, full genome HBV strains from Tunisia. Viral load quantification and phylogenetic analyses of full genome or pre-S/S sequences were performed on 196 hepatitis B surface antigen (HBsAg)-positive plasma samples from Tunisian blood donors. The median viral load was 64.65 IU ml (range <5–7.7×10 IU ml) and 89 % of samples had viral loads below 10 000 IU ml. Fifty-nine strains formed a novel subgenotype D7, 41 strains clustered in subgenotype D1, seven strains in subgenotype A2 and one strain in genotype C. The novel subgenotype D7 was defined by maximum Bayesian posterior probability, a genetic divergence from other HBV/D subgenotypes by >4 % and a stronger HBV/E signal in the X to core genes than subgenotype D1. In conclusion, HBV/D is dominant in asymptomatic Tunisian HBsAg carriers and a novel subgenotype, D7, was the most common subgenotype found in this population.

  • Received:
  • Accepted:
  • Published Online:
SGM
Loading

Article metrics loading...

/content/journal/jgv/10.1099/vir.0.009738-0
2009-07-01
2024-04-25
Loading full text...

Full text loading...

/deliver/fulltext/jgv/90/7/1622.html?itemId=/content/journal/jgv/10.1099/vir.0.009738-0&mimeType=html&fmt=ahah

References

  1. Arauz-Ruiz P., Norder H., Robertson B. H., Magnius L. O. 2002; Genotype H: a new Amerindian genotype of hepatitis B virus revealed in Central America. J Gen Virol 83:2059–2073
     [Google Scholar]
  2. Ayed K., Gorgi Y., Ayed-Jendoubi S., Aouadi H., Sfar I., Najjar T., Ben Abdallah T. 2007; Hepatitis B virus genotypes and precore/core-promoter mutations in Tunisian patients with chronic hepatitis B virus infection. J Infect 54:291–297 [CrossRef]
     [Google Scholar]
  3. Bahri O., Cheikh I., Hajji N., Djebbi A., Maamouri N., Sadraoui A., Mami N. B., Triki H. 2006; Hepatitis B genotypes, precore and core promoter mutants circulating in Tunisia. J Med Virol 78:353–357 [CrossRef]
     [Google Scholar]
  4. Bartholomeusz A., Schaefer S. 2004; Hepatitis B virus genotypes: comparison of genotyping methods. Rev Med Virol 14:3–16 [CrossRef]
     [Google Scholar]
  5. Bekondi C., Olinger C. M., Boua N., Talarmin A., Muller C. P., Le Faou A., Venard V. 2007; Central African Republic is part of the West-African hepatitis B virus genotype E crescent. J Clin Virol 40:31–37 [CrossRef]
     [Google Scholar]
  6. Bekondi C., Olinger C. M., Boua N., Talarmin A., Venard V., Muller C. P., Le Faou A. 2008; Characterization of hepatitis B virus strains from the Central African Republic: preliminary results. Pathol Biol (Paris) 56:310–313 [CrossRef]
     [Google Scholar]
  7. Borchani-Chabchoub I., Gargouri A., Mokdad-Gargouri R. 2000; Genotyping of Tunisian hepatitis B virus isolates based on the sequencing of preS2 and S regions. Microbes Infect 2:607–612 [CrossRef]
     [Google Scholar]
  8. Candotti D., Opare-Sem O., Rezvan H., Sarkodie F., Allain J. P. 2006; Molecular and serological characterization of hepatitis B virus in deferred Ghanaian blood donors with and without elevated alanine aminotransferase. J Viral Hepat 13:715–724 [CrossRef]
     [Google Scholar]
  9. Candotti D., Danso K., Allain J. P. 2007; Maternofetal transmission of hepatitis B virus genotype E in Ghana, west Africa. J Gen Virol 88:2686–2695 [CrossRef]
     [Google Scholar]
  10. Candotti D., Grabarczyk P., Ghiazza P., Roig R., Casamitjana N., Iudicone P., Schmidt M., Bird A., Crookes R. other authors 2008; Characterization of occult hepatitis B virus from blood donors carrying genotype A2 or genotype D strains. J Hepatol 49:537–547 [CrossRef]
     [Google Scholar]
  11. Coursaget P., Gharbi Y., Khrouf N., Depril N., Boukhris N., Fritzell B., Kastally R. 1994; Familial clustering of hepatitis B virus infections and prevention of perinatal transmission by immunization with a reduced number of doses in an area of intermediate endemicity (Tunisia. Vaccine 12:275–278 [CrossRef]
     [Google Scholar]
  12. Hannachi N., Bahri O., Mhalla S., Marzouk M., Sadraoui A., Belguith A., Triki H., Boukadida J. 2009; Hepatitis B virus infection in Tunisian pregnant women: risk factors and viral DNA levels in HBe antigen negative women. Pathol Biol (Paris) 57:e43–e47 (in French) [CrossRef]
     [Google Scholar]
  13. Huelsenbeck J. P., Ronquist F. 2001; MrBayes: Bayesian inference of phylogeny. Bioinformatics 17:754–755 [CrossRef]
     [Google Scholar]
  14. Khelifa F., Thibault V. 2008; Characteristics of hepatitis B viral strains in chronic carrier patients from North-East Algeria. Pathol Biol (Paris) 57:107–113 (in French)
    [Google Scholar]
  15. Kimura M. 1980; A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16:111–120 [CrossRef]
     [Google Scholar]
  16. Kramvis A., Kew M. C. 2007; Epidemiology of hepatitis B virus in Africa, its genotypes and clinical associations of genotypes. Hepatol Res 37:S9–S19 [CrossRef]
     [Google Scholar]
  17. Kramvis A., Arakawa K., Yu M. C., Nogueira R., Stram D. O., Kew M. C. 2008; Relationship of serological subtype, basic core promoter and precore mutations to genotypes/subgenotypes of hepatitis B virus. J Med Virol 80:27–46 [CrossRef]
     [Google Scholar]
  18. Laoi B. N., Crowley B. 2008; Molecular characterization of hepatitis B virus (HBV) isolates, including identification of a novel recombinant, in patients with acute HBV infection attending an Irish hospital. J Med Virol 80:1554–1564 [CrossRef]
     [Google Scholar]
  19. Lole K. S., Bollinger R. C., Paranjape R. S., Gadkari D., Kulkarni S. S., Novak N. G., Ingersoll R., Sheppard H. W., Ray S. C. 1999; Full-length human immunodeficiency virus type 1 genomes from subtype C-infected seroconverters in India, with evidence of intersubtype recombination. J Virol 73:152–160
     [Google Scholar]
  20. Norder H., Courouce A. M., Magnius L. O. 1992; Molecular basis of hepatitis B virus serotype variations within the four major subtypes. J Gen Virol 73:3141–3145 [CrossRef]
     [Google Scholar]
  21. Norder H., Courouce A. M., Magnius L. O. 1994; Complete genomes, phylogenetic relatedness, and structural proteins of six strains of the hepatitis B virus, four of which represent two new genotypes. Virology 198:489–503 [CrossRef]
     [Google Scholar]
  22. Norder H., Courouce A. M., Coursaget P., Echevarria J. M., Lee S. D., Mushahwar I. K., Robertson B. H., Locarnini S., Magnius L. O. 2004; Genetic diversity of hepatitis B virus strains derived worldwide: genotypes, subgenotypes, and HBsAg subtypes. Intervirology 47:289–309 [CrossRef]
     [Google Scholar]
  23. Ronquist F., Huelsenbeck J. P. 2003; MrBayes 3: Bayesian phylogenetic inference under mixed models. Bioinformatics 19:1572–1574 [CrossRef]
     [Google Scholar]
  24. Saudy N., Sugauchi F., Tanaka Y., Suzuki S., Aal A. A., Zaid M. A., Agha S., Mizokami M. 2003; Genotypes and phylogenetic characterization of hepatitis B and delta viruses in Egypt. J Med Virol 70:529–536 [CrossRef]
     [Google Scholar]
  25. Simmonds P., Midgley S. 2005; Recombination in the genesis and evolution of hepatitis B virus genotypes. J Virol 79:15467–15476 [CrossRef]
     [Google Scholar]
  26. Stuyver L., De Gendt S., Van Geyt C., Zoulim F., Fried M., Schinazi R. F., Rossau R. 2000; A new genotype of hepatitis B virus: complete genome and phylogenetic relatedness. J Gen Virol 81:67–74
     [Google Scholar]
  27. Swofford D. L. 2002 paup*: phylogenetic analysis using parsimony (and other methods), version 4.0b10 Sunderland, MA: Sinauer Associates;
     [Google Scholar]
  28. Triki H., Said N., Ben Salah A., Arrouji A., Ben Ahmed F., Bouguerra A., Hmida S., Dhahri R., Dellagi K. 1997; Seroepidemiology of hepatitis B, C and delta viruses in Tunisia. Trans R Soc Trop Med Hyg 91:11–14 [CrossRef]
     [Google Scholar]
  29. Triki H., Ben Slimane S., Ben Mami N., Sakka T., Ben Ammar A., Dellagi K. 2000; High circulation of hepatitis B virus (HBV) precore mutants in Tunisia, North Africa. Epidemiol Infect 125:169–174 [CrossRef]
     [Google Scholar]
  30. Zahn A., Li C., Danso K., Candotti D., Owusu-Ofori S., Temple J., Allain J. P. 2008; Molecular characterization of occult hepatitis B virus in genotype E-infected subjects. J Gen Virol 89:409–418 [CrossRef]
     [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/vir.0.009738-0
Loading
A novel hepatitis B virus subgenotype, D7, in Tunisian blood donors
J. Gen. Virol. 90, 1622 (2009); https://doi.org/10.1099/vir.0.009738-0
/content/journal/jgv/10.1099/vir.0.009738-0
/content/journal/jgv/10.1099/vir.0.009738-0
Loading

Data & Media loading...

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error