1887

Abstract

The anti-lipopolysaccharide factor (ALF) from the black tiger shrimp, , has been shown previously to exhibit a broad spectrum of activity against various strains of bacteria and fungi. Herein, the recombinant ALF3 (rALF3) protein was examined for its role in the defence against white spot syndrome virus (WSSV) infection in haematopoietic (Hpt) cell cultures of the freshwater crayfish, , as well as in live shrimps. Incubation of Hpt cell cultures with a mixture of WSSV and rALF3 resulted in a dose-dependent decrease in VP28 gene expression levels, compared with those incubated with WSSV alone, with an rALF3 IC value lower than 2.5 μM. However, pre-treatment of Hpt cells with 5 μM rALF3 showed no induced protection against subsequent WSSV infection, whereas the synthetic crayfish ALF peptide could protect cells at a higher concentration (10 μM). The role of ALF3 was examined by injection of with WSSV pre-treated with rALF3 protein. The results clearly showed that rALF3 was able to reduce WSSV propagation and prolong the survival of shrimps.

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2009-06-01
2019-11-15
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