1887

Abstract

In a previous study, we cloned 27 discrete genome segments of bracovirus (CpBV) and provided the complete nucleotide sequences and annotation. Seven putative coding regions were predicted from one of the largest segments, CpBV-S30. The activity of promoters associated with six predicted ORFs from this segment were investigated using both transient and baculovirus expression assays with enhanced green fluorescent protein as a reporter gene. CpBV promoters showed activity earlier than the promoter and the activity of some of these promoters was superior to that of the multiple nucleopolyhedrovirus (AcMNPV) promoter in the baculovirus expression assays. The promoter of ORF3004 showed the highest level of activity in insect cells, exhibiting 24 % of the activity obtained with the AcMNPV promoter in Sf9 cells. In larvae, the ORF3006 promoter showed the highest activity, with about 35 % of the activity measured with the promoter. In addition, analysis of the ORF3006 promoter revealed that the region between −382 and −422 from the translation start point was critical for activity of this promoter. These results suggest that the CpBV-S30 promoters characterized here could be useful tools in a variety of biotechnological applications, such as gene expression analyses and insecticide development.

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2009-05-01
2019-11-21
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References

  1. Asgari, S. & Schmidt, O. ( 2001; ). Promoter studies of a polydnavirus gene from Cotesia rubecula (Hym: Braconidae). Arch Virol 146, 1979–1989.[CrossRef]
    [Google Scholar]
  2. Asgari, S., Hellers, M. & Schmidt, O. ( 1996; ). Host haemocyte inactivation by an insect parasitoid: transient expression of a polydnavirus gene. J Gen Virol 77, 2653–2662.[CrossRef]
    [Google Scholar]
  3. Asgari, S., Schmidt, O. & Theopold, U. ( 1997; ). A polydnavirus-encoded protein of an endoparasitoid wasp is an immune suppressor. J Gen Virol 78, 3061–3070.
    [Google Scholar]
  4. Bae, S. & Kim, Y. ( 2004; ). Host physiological changes due to parasitism of a braconid wasp, Cotesia plutellae, on diamondback moth, Plutella xylostella. Comp Biochem Physiol A Mol Integr Physiol 138, 39–44.[CrossRef]
    [Google Scholar]
  5. Beckage, N. E. ( 1993; ). Games parasites play: the dynamic roles of peptides and proteins in the host–parasite interaction. In Parasites and Pathogens of Insects, vol. 1, Parasites, pp. 25–58. Edited by N. E. Beckage, S. N. Thompson & B. A. Federici. New York: Academic Press.
  6. Beckage, N. E. & Gelman, D. B. ( 2004; ). Wasp parasitoid disruption of host development: implications for new biologically based strategies for insect control. Annu Rev Entomol 49, 299–330.[CrossRef]
    [Google Scholar]
  7. Beckage, N. E., Templeton, T. J., Nielsen, B. D., Cook, D. I. & Stoltz, D. B. ( 1987; ). Parasitism-induced hemolymph polypeptides in Manduca sexta (L.) larvae parasitized by the braconid wasp Cotesia congregata (Say). Insect Biochem 17, 439–455.[CrossRef]
    [Google Scholar]
  8. Choi, J. Y., Roh, J. Y., Kang, J. N., Shim, H. J., Woo, S. D., Jin, B. R., Li, M. S. & Je, Y. H. ( 2005; ). Genomic segments cloning and analysis of Cotesia plutellae polydnavirus using plasmid capture system. Biochem Biophys Res Commun 332, 487–493.[CrossRef]
    [Google Scholar]
  9. Choi, J. Y., Wang, Y., Kim, Y.-S., Kang, J. N., Roh, J. Y., Woo, S.-D., Jin, B. R. & Je, Y. H. ( 2008; ). Insecticidal activities of recombinant Autographa californica nucleopolyhedrovirus containing a scorpion neurotoxin gene using promoters from Cotesia plutellae bracovirus. J Asia Pac Entomol 11, 155–159.[CrossRef]
    [Google Scholar]
  10. Choudary, P. V., Kamita, S. G. & Maeda, S. ( 1995; ). Expression of foreign genes in Bombyx mori larvae using baculovirus vectors. In Baculovirus Expression Protocols, pp. 243–264. Edited by C. D. Richardson. Totowa, NJ: Humana Press.
  11. Doucet, D., Levasseur, A., Béliveau, C., Lapointe, R., Stoltz, D. & Cusson, M. ( 2007; ). In vitro integration of an ichnovirus genome segment into the genomic DNA of lepidopteran cells. J Gen Virol 88, 105–113.[CrossRef]
    [Google Scholar]
  12. Fleming, J. A. ( 1992; ). Polydnaviruses: mutualists and pathogens. Annu Rev Entomol 37, 401–425.[CrossRef]
    [Google Scholar]
  13. Gad, W. & Kim, Y. ( 2008; ). A viral histone H4 encoded by Cotesia plutellae bracovirus inhibits haemocyte-spreading behaviour of the diamondback moth, Plutella xylostella. J Gen Virol 89, 931–938.[CrossRef]
    [Google Scholar]
  14. Gundersen-Rindal, D. & Dougherty, E. M. ( 2000; ). Evidence for integration of Glyptapanteles indiensis polydnavirus DNA into the chromosome of Lymantria dispar in vitro. Virus Res 66, 27–37.[CrossRef]
    [Google Scholar]
  15. Gundersen-Rindal, D. E. & Lynn, D. E. ( 2003; ). Polydnavirus integration in lepidopteran host cells in vitro. J Insect Physiol 49, 453–462.[CrossRef]
    [Google Scholar]
  16. Gundersen-Rindal, D., Lynn, D. E. & Dougherty, E. M. ( 1999; ). Transformation of lepidopteran and coleopteran insect cell lines by Glyptapanteles indiensis polydnavirus DNA. In Vitro Cell Dev Biol Anim 35, 111–114.[CrossRef]
    [Google Scholar]
  17. Harwood, S. H. & Beckage, N. E. ( 1994; ). Purification and characterization of an early-expressed polydnavirus-induced protein from the hemolymph of Manduca sexta larvae parasitized by Cotesia congregate. Insect Biochem Mol Biol 24, 685–698.[CrossRef]
    [Google Scholar]
  18. Harwood, S. H., Grosovsky, A. J., Cowles, E. A., Davis, J. W. & Beckage, N. E. ( 1994; ). An abundantly expressed hemolymph glycoprotein isolated from newly parasitized Manduca sexta larvae is a polydnavirus gene product. Virology 205, 381–392.[CrossRef]
    [Google Scholar]
  19. Hayakawa, Y. ( 1990; ). Juvenile hormone esterase activity repressive factor in the plasma of parasitized insect larvae. J Biol Chem 265, 10813–10816.
    [Google Scholar]
  20. Hayakawa, Y. ( 1994; ). Cellular immunosuppressive protein in the plasma of parasitized insect larvae. J Biol Chem 269, 14536–14540.
    [Google Scholar]
  21. Hayakawa, Y. ( 1995; ). Growth-blocking peptide: an insect biogenic peptide that prevents the onset of metamorphosis. J Insect Physiol 41, 1–6.[CrossRef]
    [Google Scholar]
  22. Hayakawa, Y., Yazaki, K., Yamanaka, A. & Tanaka, T. ( 1994; ). Expression of polydnavirus genes from the parasitoid wasp Cotesia kariyai in two noctuid hosts. Insect Mol Biol 3, 97–103.[CrossRef]
    [Google Scholar]
  23. Je, Y. H., Chang, J. H., Roh, J. Y. & Jin, B. R. ( 2001; ). Generation of baculovirus expression vector using defective Autographa californica nuclear polyhedrosis virus genome maintained in Escherichia coli for Occ+ virus production. Int J Indust Entomol 2, 155–160.
    [Google Scholar]
  24. Kim, M. K., Sisson, G. & Stoltz, D. ( 1996; ). Ichnovirus infection of an established gypsy moth cell line. J Gen Virol 77, 2321–2328.[CrossRef]
    [Google Scholar]
  25. Kim, Y., Bae, S. & Lee, S. ( 2004; ). Polydnavirus replication and ovipositional habit of Cotesia plutellae. Korean J Appl Entomol 43, 225–231.
    [Google Scholar]
  26. Kovacs, G. R., Guarino, L. A. & Summers, M. D. ( 1991; ). Novel regulatory properties of the IE1 and IE0 transactivators encoded by the baculovirus Autographa californica multicapsid nuclear polyhedrosis virus. J Virol 65, 5281–5288.
    [Google Scholar]
  27. Lavine, M. D. & Beckage, N. E. ( 1995; ). Polydnaviruses: potent mediators of host insect immune dysfunction. Parasitol Today 11, 368–378.[CrossRef]
    [Google Scholar]
  28. Lawrence, P. O. & Lanzrein, B. ( 1993; ). Hormonal interactions between insect endoparasite and their host insects. In Parasites and Pathogens of Insects, vol. 1, Parasites, pp. 59–86. Edited by N. E. Beckage, S. N. Thompson & B. A. Federici. New York: Academic Press.
  29. Le, N. T., Asgari, S., Amaya, K., Tan, F. F. & Beckage, N. E. ( 2003; ). Persistence and expression of Cotesia congregata polydnavirus in host larvae of the tobacco hornworm, Manduca sexta. J Insect Physiol 49, 533–543.[CrossRef]
    [Google Scholar]
  30. Lee, S. & Kim, Y. ( 2004; ). Juvenile hormone esterase of diamondback moth, Plutella xylostella, and parasitism of Cotesia plutellae. J Asia Pac Entomol 7, 283–287.[CrossRef]
    [Google Scholar]
  31. Lee, S., Basio, N. A., Kim, D. S. & Kim, Y. ( 2005; ). Proteomic analysis of parasitization by Cotesia plutellae against diamondback moth, Plutella xylostella. J Asia Pac Entomol 8, 53–60.[CrossRef]
    [Google Scholar]
  32. McKelvey, T. A., Lynn, D. E., Gundersen-Rindal, D., Guzo, D., Stoltz, D. A., Guthrie, K. P., Taylor, P. B. & Dougherty, E. M. ( 1996; ). Transformation of gypsy moth (Lymantria dispar) cell lines by infection with Glyptapanteles indiensis polydnavirus. Biochem Biophys Res Commun 225, 764–770.[CrossRef]
    [Google Scholar]
  33. Olson, V. A., Wetter, J. A. & Friesen, P. D. ( 2002; ). Baculovirus transregulator IE1 requires a dimeric nuclear localization element for nuclear import and promoter activation. J Virol 76, 9505–9515.[CrossRef]
    [Google Scholar]
  34. O'Reilly, D. R., Miller, L. K. & Luckow, V. A. ( 1992; ). Baculovirus Expression Vectors: A Laboratory Manual. New York: Oxford University Press.
  35. Schuler, T. H., Denholm, I., Clark, S. J., Stewart, C. N. & Poppy, G. M. ( 2004; ). Effects of Bt plants on the development and survival of the parasitoid Cotesia plutellae (Hymenoptera: Braconidae) in susceptible and Bt-resistant larvae of the diamondback moth, Plutella xylostella (Lepidoptera: Plutellidae). J Insect Physiol 50, 435–443.[CrossRef]
    [Google Scholar]
  36. Shippam-Brett, C. E., Willis, L. G. & Theilmann, D. A. ( 2001; ). Analysis of sequences involved in IE2 transactivation of a baculovirus immediate-early gene promoter and identification of a new regulatory motif. Virus Res 75, 13–28.[CrossRef]
    [Google Scholar]
  37. Stettler, P., Trenczek, T., Wyler, T., Pfister-Wilhelm, R. & Lanzrein, B. ( 1998; ). Overview of parasitism associated effects on host haemocytes in larval parasitoids and comparison with effects of the egg-larval parasitoid Chelonus inanitus on its host Spodoptera littoralis. J Insect Physiol 44, 817–831.[CrossRef]
    [Google Scholar]
  38. Stoltz, D. B. ( 1993; ). Polydnavirus life cycle. In Parasites and Pathogens of Insects, vol. 1, Parasites, pp. 167–187. Edited by N. E. Beckage, S. N. Thompson & B. A. Federici. New York: Academic Press.
  39. Stoltz, D. B. & Vinson, S. B. ( 1979; ). Viruses and parasitism in insects. Adv Virus Res 24, 125–171.
    [Google Scholar]
  40. Strand, M. R. & Pech, L. L. ( 1995; ). Microplitis demolitor polydnavirus induces apoptosis of a specific haemocyte morphotype in Pseudoplusia includens. J Gen Virol 76, 283–291.[CrossRef]
    [Google Scholar]
  41. Strand, M. R., McKenzie, D. I., Grassl, V., Dover, B. A. & Aiken, J. M. ( 1992; ). Persistence and expression of Microplitis demolitor polydnavirus in Pseudoplusia includens. J Gen Virol 73, 1627–1635.[CrossRef]
    [Google Scholar]
  42. Summers, M. D. & Dib-Hajj, S. D. ( 1995; ). Polydnavirus-facilitated endoparasite protection against host immune defenses. Proc Natl Acad Sci U S A 92, 29–36.[CrossRef]
    [Google Scholar]
  43. Theilmann, D. A. & Summers, M. D. ( 1986; ). Molecular analysis of Campoletis sonorensis virus DNA in the lepidopteran host Heliothis virescens. J Gen Virol 67, 1961–1969.[CrossRef]
    [Google Scholar]
  44. Turner, B. M. ( 1995; ). Histone H4, the cell cycle and a question of integrity. Bioessays 17, 1013–1015.[CrossRef]
    [Google Scholar]
  45. Vinson, S. B. ( 1990; ). How parasitoids deal with the immune system of their host: an overview. Arch Insect Biochem Physiol 13, 3–27.[CrossRef]
    [Google Scholar]
  46. Volkoff, A. N., Cérutti, P., Rocher, J., Ohresser, M. C., Devauchelle, G. & Duonor-Cérutti, M. ( 1999; ). Related RNAs in lepidopteran cells after in vitro infection with Hyposoter didymator virus define a new polydnavirus gene family. Virology 263, 349–363.[CrossRef]
    [Google Scholar]
  47. Volkoff, A. N., Rocher, J., Cérutti, P., Ohresser, M. C., d'Aubenton-Carafa, Y., Devauchelle, G. & Duonor-Cérutti, M. ( 2001; ). Persistent expression of a newly characterized Hyposoter didymator polydnavirus gene in long-term infected lepidopteran cell lines. J Gen Virol 82, 963–969.
    [Google Scholar]
  48. Webb, B. A. ( 1998; ). Polydnavirus biology, genome structure, and evolution. In The Insect Viruses, pp. 105–139. Edited by L. K. Miller & L. A. Ball. New York: Plenum.
  49. Wyler-Duda, P., Bernard, V., Stadler, M., Suter, D. & Schümperli, D. ( 1997; ). Histone H4 mRNA from the nematode Ascaris lumbricoides is cis-spliced and polyadenylated. Biochim Biophys Acta 1350, 259–261.[CrossRef]
    [Google Scholar]
  50. Yamanaka, A., Hayakawa, Y., Noda, H., Nakashima, N. & Watanabe, H. ( 1996; ). Characterization of polydnavirus-encoded mRNA in parasitized armyworm larvae. Insect Biochem Mol Biol 26, 529–536.[CrossRef]
    [Google Scholar]
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