1887

Abstract

Poxviruses encode a large family of ankyrin-repeat (ANK) proteins, most of which contain an F-box-like motif necessary for the interaction of the ANK proteins with SCF1 (Skp1–Cullin1–F-box) complexes. The viral motif is generally truncated compared with the three--helix cellular F-box. Cellular F-box -helices 1–3 and regions C-terminal to them have been shown to contribute to Skp1 binding. We report that the poxvirus F-boxes generally contain only two -helices, corresponding to cellular F-box -helices 1 and 2. A third -helix was detected in some poxvirus F-boxes, but was not predicted to interact with Skp1. All but one of the poxvirus ANK/F-box proteins examined terminated directly after the F-box, excluding any contribution by C-terminal regions to the binding of Skp1. Here we show that, despite this truncation, the F-box of a prototypical poxvirus ANK protein, containing two -helices, is not only necessary but also sufficient for interaction with SCF1.

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2009-05-01
2021-10-19
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References

  1. Arnold K., Bordoli L., Kopp J., Schwede T. 2006; The swiss-model workspace: a web-based environment for protein structure homology modelling. Bioinformatics 22:195–201 [CrossRef]
    [Google Scholar]
  2. Bai C., Sen P., Hofmann K., Ma L., Goebl M., Harper J. W., Elledge S. J. 1996; SKP1 connects cell cycle regulators to the ubiquitin proteolysis machinery through a novel motif, the F-box. Cell 86:263–274 [CrossRef]
    [Google Scholar]
  3. Bosu D. R., Kipreos E. T. 2008; Cullin-RING ubiquitin ligases: global regulation and activation cycles. Cell Div 3: 7 [CrossRef]
    [Google Scholar]
  4. Cole C., Barber J. D., Barton G. J. 2008; The Jpred 3 secondary structure prediction server. Nucleic Acids Res 36:W197–W201 [CrossRef]
    [Google Scholar]
  5. DeLano W. L. 2007 MacPyMOL: a PyMOL-based molecular graphics application for Mac OS X Palo Alto, FL: DeLano Scientific LLC;
    [Google Scholar]
  6. Galan J. M., Peter M. 1999; Ubiquitin-dependent degradation of multiple F-box proteins by an autocatalytic mechanism. Proc Natl Acad Sci U S A 96:9124–9129 [CrossRef]
    [Google Scholar]
  7. Guex N., Peitsch M. C. 1997; swiss-model and the Swiss-PdbViewer: an environment for comparative protein modeling. Electrophoresis 18:2714–2723 [CrossRef]
    [Google Scholar]
  8. Hao B., Zheng N., Schulman B. A., Wu G., Miller J. J., Pagano M., Pavletich N. P. 2005; Structural basis of the Cks1-dependent recognition of p27(Kip1) by the SCF(Skp2) ubiquitin ligase. Mol Cell 20:9–19 [CrossRef]
    [Google Scholar]
  9. Hao B., Oehlmann S., Sowa M. E., Harper J. W., Pavletich N. P. 2007; Structure of a Fbw7–Skp1–cyclin E complex: multisite-phosphorylated substrate recognition by SCF ubiquitin ligases. Mol Cell 26:131–143 [CrossRef]
    [Google Scholar]
  10. Johnston J. B., Wang G., Barrett J. W., Nazarian S. H., Colwill K., Moran M., McFadden G. 2005; Myxoma virus M-T5 protects infected cells from the stress of cell cycle arrest through its interaction with host cell cullin-1. J Virol 79:10750–10763 [CrossRef]
    [Google Scholar]
  11. Kipreos E. T., Pagano M. 2000; The F-box protein family. Genome Biol 1:REVIEWS3002
    [Google Scholar]
  12. Mercer A. A., Fleming S. B., Ueda N. 2005; F-box-like domains are present in most poxvirus ankyrin repeat proteins. Virus Genes 31:127–133 [CrossRef]
    [Google Scholar]
  13. Orlicky S., Tang X., Willems A., Tyers M., Sicheri F. 2003; Structural basis for phosphodependent substrate selection and orientation by he SCFCdc4 ubiquitin ligase. Cell 112:243–256 [CrossRef]
    [Google Scholar]
  14. Schulman B. A., Carrano A. C., Jeffrey P. D., Bowen Z., Kinnucan E. R., Finnin M. S., Elledge S. J., Harper J. W., Pagano M., Pavletich N. P. 2000; Insights into SCF ubiquitin ligases from the structure of the Skp1–Skp2 complex. Nature 408:381–386 [CrossRef]
    [Google Scholar]
  15. Seet B. T., Johnston J. B., Brunetti C. R., Barrett J. W., Everett H., Cameron C., Sypula J., Nazarian S. H., Lucas A., McFadden G. 2003; Poxviruses and immune evasion. Annu Rev Immunol 21:377–423 [CrossRef]
    [Google Scholar]
  16. Skowyra D., Craig K. L., Tyers M., Elledge S. J., Harper J. W. 1997; F-box proteins are receptors that recruit phosphorylated substrates to the SCF ubiquitin-ligase complex. Cell 91:209–219 [CrossRef]
    [Google Scholar]
  17. Sonnberg S., Seet B. T., Pawson T., Fleming S. B., Mercer A. A. 2008; Poxvirus ankyrin repeat proteins are a unique class of F-box proteins that associate with cellular SCF1 ubiquitin ligase complexes. Proc Natl Acad Sci U S A 105:10955–10960 [CrossRef]
    [Google Scholar]
  18. Sperling K. M., Schwantes A., Schnierle B. S., Sutter G. 2008; The highly conserved orthopoxvirus 68k ankyrin-like protein is part of a cellular SCF ubiquitin ligase complex. Virology 374:234–239 [CrossRef]
    [Google Scholar]
  19. Tan X., Calderon-Villalobos L. I., Sharon M., Zheng C., Robinson C. V., Estelle M., Zheng N. 2007; Mechanism of auxin perception by the TIR1 ubiquitin ligase. Nature 446:640–645 [CrossRef]
    [Google Scholar]
  20. van Buuren N., Couturier B., Xiong Y., Barry M. 2008; Ectromelia virus encodes a novel family of F-box proteins that interact with the Scf complex. J Virol 82:9917–9927 [CrossRef]
    [Google Scholar]
  21. Willems A. R., Schwab M., Tyers M. 2004; A hitchhiker's guide to the cullin ubiquitin ligases: SCF and its kin. Biochim Biophys Acta 1695133–170 [CrossRef]
    [Google Scholar]
  22. Wu G., Xu G., Schulman B. A., Jeffrey P. D., Harper J. W., Pavletich N. P. 2003; Structure of a β -TrCP1–Skp1– β -catenin complex: destruction motif binding and lysine specificity of the SCF β -TrCP1 ubiquitin ligase. Mol Cell 11:1445–1456 [CrossRef]
    [Google Scholar]
  23. Zheng N., Schulman B. A., Song L., Miller J. J., Jeffrey P. D., Wang P., Chu C., Koepp D. M., Elledge S. J. other authors 2002; Structure of the Cul1–Rbx1–Skp1-F boxSkp2 SCF ubiquitin ligase complex. Nature 416:703–709 [CrossRef]
    [Google Scholar]
  24. Zhou P., Howley P. M. 1998; Ubiquitination and degradation of the substrate recognition subunits of SCF ubiquitin-protein ligases. Mol Cell 2:571–580 [CrossRef]
    [Google Scholar]
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