1887

Abstract

Poxviruses encode a large family of ankyrin-repeat (ANK) proteins, most of which contain an F-box-like motif necessary for the interaction of the ANK proteins with SCF1 (Skp1–Cullin1–F-box) complexes. The viral motif is generally truncated compared with the three--helix cellular F-box. Cellular F-box -helices 1–3 and regions C-terminal to them have been shown to contribute to Skp1 binding. We report that the poxvirus F-boxes generally contain only two -helices, corresponding to cellular F-box -helices 1 and 2. A third -helix was detected in some poxvirus F-boxes, but was not predicted to interact with Skp1. All but one of the poxvirus ANK/F-box proteins examined terminated directly after the F-box, excluding any contribution by C-terminal regions to the binding of Skp1. Here we show that, despite this truncation, the F-box of a prototypical poxvirus ANK protein, containing two -helices, is not only necessary but also sufficient for interaction with SCF1.

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2009-05-01
2019-11-13
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vol. , part 5, pp. 1224 – 1228

-Helix predictions of poxvirus F-boxes generated through structure modelling. Alignments of three F-box consensus sequences, three cellular F-boxes and ten poxvirus F-boxes are shown as in Fig. 1. Black boxes indicate the presence of -helices either determined from crystal structures (Skp2, Fbw7, TIR1) or predicted by SWISS-MODEL structure predictions using Skp2 as template. Green boxes indicate -sheet prediction using the First Approach Mode of SWISS-MODEL.



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