1887

Abstract

The E3 ligase WSSV222 of white spot syndrome virus (WSSV) is involved in anti-apoptosis regulation by ubiquitin-mediated degradation of tumour suppressor-like protein (TSL), a shrimp tumour suppressor. In the present study, gene expression was silenced by using specific small interfering RNA (siRNA) in Sf9 and BHK cells. Based on the results of the silencing, WSSV-challenged shrimp were treated with anti- siRNA to knock down WSSV222 protein expression. The survival rate of shrimp and the efficiency of WSSV replication were assessed to evaluate the efficacy of anti- siRNA in regulating WSSV infection in shrimp. The anti- siRNA reduced the cumulative mortality in shrimp challenged with 10 copies of WSSV and delayed the mean time to death in shrimp challenged with the higher dose of 10 copies. The results of real-time quantitative PCR showed that virus replication was delayed and reduced in WSSV-challenged shrimp treated with anti- siRNA in comparison with challenged shrimp treated with random-control siRNA. Co-immunoprecipitation assays revealed that silencing inhibited the degradation of TSL in WSSV-challenged shrimp, indicating the requirement for WSSV222 for efficient replication of WSSV in shrimp.

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2009-06-01
2019-11-13
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