1887

Abstract

Infectious bursal disease virus (IBDV), an important avian pathogen, exhibits a specific tropism for immature B-lymphocyte populations. We have investigated the ability of IBDV to replicate in chicken B-lymphoid DT40 cells, a tumour cell line derived from the bursa of Fabricius of a chicken infected with avian leukosis virus. Our results show that IBDV persistently infects DT40 cells. Establishment of the persistent infection is associated with an extensive remodelling of the hypervariable region of the VP2 capsid polypeptide, accumulating 14 amino acid changes during the first 60 days of the persistent infection. The amino acid sequence of the non-structural VP5 polypeptide, involved in virus dissemination, is not altered during the persistent infection. Results described in this report constitute the first demonstration of the ability of IBDV to establish a persistent infection .

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2009-05-01
2019-11-14
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References

  1. Ahmed, R., Morrison, L. A. & Knipe, D. M. ( 1997; ). Viral persistence. In Viral Pathogenesis, pp. 181–205. Edited by N. Nathanson, R. Ahmed, F. Gonzalez-Scarano, D. E. Griffin, K. V. Holmes, F. A. Murphy & H. L. Robinson. Philadelphia, PA: Lippincott–Raven.
  2. Baba, T. W., Giroir, B. P. & Humphries, E. H. ( 1985; ). Cell lines derived from avian lymphomas exhibit two distinct phenotypes. Virology 144, 139–151.[CrossRef]
    [Google Scholar]
  3. Bayliss, C. D., Spies, U., Shaw, K., Peters, R. W., Papageorgiou, A., Müller, H. & Boursnell, M. E. G. ( 1990; ). A comparison of the sequences of segment A of four infectious bursal disease virus strains and identification of a variable region in VP2. J Gen Virol 71, 1303–1312.[CrossRef]
    [Google Scholar]
  4. Becht, H. ( 1980; ). Infectious bursal disease virus. Curr Top Microbiol Immunol 90, 107–121.
    [Google Scholar]
  5. Borzakian, S., Couderc, T., Barbier, Y., Attal, G., Pelletier, I. & Colbere-Garapin, F. ( 1992; ). Persistent poliovirus infection: establishment and maintenance involve distinct mechanisms. Virology 186, 398–408.[CrossRef]
    [Google Scholar]
  6. Buerstedde, J. M. & Takeda, S. ( 1991; ). Increased ratio of targeted to random integration after transfection of chicken B cell lines. Cell 67, 179–188.[CrossRef]
    [Google Scholar]
  7. Cursiefen, D., Käufer, I. & Becht, H. ( 1979; ). Loss of virulence in a small plaque mutant of the infectious bursal disease virus. Arch Virol 59, 39–46.[CrossRef]
    [Google Scholar]
  8. Dermody, T. S., Nibert, M. L., Wetzel, J. D., Tong, X. & Fields, B. N. ( 1993; ). Cells and viruses with mutations affecting viral entry are selected during persistent infections of L cells with mammalian reoviruses. J Virol 67, 2055–2063.
    [Google Scholar]
  9. Dobos, P., Hill, B. J., Hallett, R., Kells, D. T., Becht, H. & Teninges, D. ( 1979; ). Biophysical and biochemical characterization of five animal viruses with bisegmented double-stranded RNA genomes. J Virol 32, 593–605.
    [Google Scholar]
  10. Fernandez-Arias, A., Risco, C., Martinez, S., Albar, J. P. & Rodriguez, J. F. ( 1998; ). Expression of ORF A1 of infectious bursal disease virus results in the formation of virus-like particles. J Gen Virol 79, 1047–1054.
    [Google Scholar]
  11. Garriga, D., Querol-Audí, J., Abaitua, F., Saugar, I., Pous, J., Verdaguer, N., Castón, J. R. & Rodriguez, J. F. ( 2006; ). The 2.6-angstrom structure of infectious bursal disease virus-derived T=1 particles reveals new stabilizing elements of the virus capsid. J Virol 80, 6895–6905.[CrossRef]
    [Google Scholar]
  12. Hassan, M. K., Nielsen, C. K., Ward, L. A., Jackwood, D. J. & Saif, Y. M. ( 1996; ). Antigenicity, pathogenicity, and immunogenicity of small and large plaque infectious bursal disease virus clones. Avian Dis 40, 832–836.[CrossRef]
    [Google Scholar]
  13. Hofmann, M. A., Senanayake, S. D. & Brian, D. A. ( 1993; ). A translation-attenuating intraleader open reading frame is selected on coronavirus mRNAs during persistent infection. Proc Natl Acad Sci U S A 90, 11733–11737.[CrossRef]
    [Google Scholar]
  14. Kibenge, F. S., Dhillon, A. S. & Russell, R. G. ( 1988; ). Biochemistry and immunology of infectious bursal disease virus. J Gen Virol 69, 1757–1775.[CrossRef]
    [Google Scholar]
  15. Lange, H., Müller, H., Käufer, I. & Becht, H. ( 1987; ). Pathogenic and structural properties of wild type infectious bursal disease virus (IBDV) and virus grown in vitro. Arch Virol 92, 187–196.[CrossRef]
    [Google Scholar]
  16. Lin, T.-W., Lo, C.-W., Lai, S.-Y., Fan, R.-J., Lo, C.-J., Chou, Y.-m., Thiruvengadam, R., Wang, A. H. J. & Wang, M.-Y. ( 2007; ). Chicken heat shock protein 90 is a component of the putative cellular receptor complex of infectious bursal disease virus. J Virol 81, 8730–8741.[CrossRef]
    [Google Scholar]
  17. Lombardo, E., Maraver, A., Caston, J. R., Rivera, J., Fernandez-Arias, A., Serrano, A., Carrascosa, J. L. & Rodriguez, J. F. ( 1999; ). VP1, the putative RNA-dependent RNA polymerase of infectious bursal disease virus, forms complexes with the capsid protein VP3, leading to efficient encapsidation into virus-like particles. J Virol 73, 6973–6983.
    [Google Scholar]
  18. Lombardo, E., Maraver, A., Espinosa, I., Fernandez-Arias, A. & Rodriguez, J. F. ( 2000; ). VP5, the nonstructural polypeptide of infectious bursal disease virus, accumulates within the host plasma membrane and induces cell lysis. Virology 277, 345–357.[CrossRef]
    [Google Scholar]
  19. Luque, D., Saugar, I., Rodríguez, J. F., Verdaguer, N., Garriga, D., Martín, C. S., Velázquez-Muriel, J. A., Trus, B. L., Carrascosa, J. L. & Castón, J. R. ( 2007; ). Infectious bursal disease virus capsid assembly and maturation by structural rearrangements of a transient molecular switch. J Virol 81, 6869–6878.[CrossRef]
    [Google Scholar]
  20. Mundt, E., Kollner, B. & Kretzschmar, D. ( 1997; ). VP5 of infectious bursal disease virus is not essential for viral replication in cell culture. J Virol 71, 5647–5651.
    [Google Scholar]
  21. Nieper, H. & Muller, H. ( 1996; ). Susceptibility of chicken lymphoid cells to infectious bursal disease virus does not correlate with the presence of specific binding sites. J Gen Virol 77, 1229–1237.[CrossRef]
    [Google Scholar]
  22. Ogawa, M., Yamaguchi, T., Setiyono, A., Ho, T., Matsuda, H., Furusawa, S., Fukushi, H. & Hirai, K. ( 1998; ). Some characteristics of a cellular receptor for virulent infectious bursal disease virus by using flow cytometry. Arch Virol 143, 2327–2341.[CrossRef]
    [Google Scholar]
  23. Schnitzler, D., Bernstein, F., Muller, H. & Becht, H. ( 1993; ). The genetic basis for the antigenicity of the VP2 protein of the infectious bursal disease virus. J Gen Virol 74, 1563–1571.[CrossRef]
    [Google Scholar]
  24. Setiyono, A., Hayashi, T., Yamaguchi, T., Fukushi, H. & Hirai, K. ( 2001; ). Detection of cell membrane proteins that interact with virulent infectious bursal disease virus. J Vet Med Sci 63, 219–221.[CrossRef]
    [Google Scholar]
  25. Terasaki, K., Hirayama, H., Kasanga, C. J., Maw, M. T., Ohya, K., Yamaguchi, T. & Fukushi, H. ( 2008; ). Chicken B lymphoma DT40 cells as a useful tool for in vitro analysis of pathogenic infectious bursal disease virus. J Vet Med Sci 70, 407–410.[CrossRef]
    [Google Scholar]
  26. Winding, P. & Berchtold, M. W. ( 2001; ). The chicken B cell line DT40: a novel tool for gene disruption experiments. J Immunol Methods 249, 1–16.[CrossRef]
    [Google Scholar]
  27. Yamaguchi, T., Kondo, T., Inoshima, Y., Ogawa, M., Miyoshi, M., Yanai, T., Masegi, T., Fukushi, H. & Hirai, K. ( 1996a; ). In vitro attenuation of highly virulent infectious bursal disease virus: some characteristics of attenuated strains. Avian Dis 40, 501–509.[CrossRef]
    [Google Scholar]
  28. Yamaguchi, T., Ogawa, M., Inoshima, Y., Miyoshi, M., Fukushi, H. & Hirai, K. ( 1996b; ). Identification of sequence changes responsible for the attenuation of highly virulent infectious bursal disease virus. Virology 223, 219–223.[CrossRef]
    [Google Scholar]
  29. Yao, K., Goodwin, M. A. & Vakharia, V. N. ( 1998; ). Generation of a mutant infectious bursal disease virus that does not cause bursal lesions. J Virol 72, 2647–2654.
    [Google Scholar]
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