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Journal of General Virology header logo Journal of General Virology

Volume 90, Issue 4

multiple nucleopolyhedrovirus and multiple nucleopolyhedrovirus genes are expressed as pre-proenzymes No Access

Abstract

Intracellular processing and trafficking of the baculovirus expressed cathepsin (V-CATH), which lacks canonical targeting signals, are poorly understood. The cathepsins of multiple nucleopolyhedrovirus (AcMNPV), multiple nucleopolyhedrovirus (CfMNPV) and most other alphabaculovirus group I nucleopolyhedroviruses have well-conserved N-termini containing overlapping chymotrypsin-cleavage (Y) and myristoylation (G) motifs, which are suggestive of proteolytic signal-peptide cleavage to generate proV-CATH and subsequent acylation. To determine proteolytic N-terminal processing of V-CATH, haemagglutinin epitope-coding tags were fused to the 5′ and/or 3′ ends of AcMNPV and CfMNPV . Immunoblot analysis suggested that a small N-terminal peptide is cleaved for both viruses, indicating that is expressed as a pre-proenzyme. The two viral homologues undergo similar proteolytic processing, but have different glycosylation or other post-translational modifications. An AcMNPV V-CATH–DsRED fusion protein co-localized to the endoplasmic reticulum with an HDEL motif-containing green fluorescent protein. Based on these findings, pre-proV-CATH processing and trafficking mechanisms are postulated.

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/content/journal/jgv/10.1099/vir.0.007740-0
2009-04-01
2025-01-16
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Autographa californica multiple nucleopolyhedrovirus and Choristoneura fumiferana multiple nucleopolyhedrovirus v-cath genes are expressed as pre-proenzymes
J. Gen. Virol. 90, 995 (2009); https://doi.org/10.1099/vir.0.007740-0
/content/journal/jgv/10.1099/vir.0.007740-0
/content/journal/jgv/10.1099/vir.0.007740-0
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