1887

Abstract

Two sets of infectious cDNA clones of raspberry bushy dwarf virus (RBDV) have been constructed, enabling either the synthesis of infectious RNA transcripts or the delivery of infectious binary plasmid DNA by infiltration of . In whole plants and in protoplasts, inoculation of RBDV RNA1 and RNA2 transcripts led to a low level of infection, which was greatly increased by the addition of RNA3, a subgenomic RNA coding for the RBDV coat protein (CP). Agroinfiltration of RNA1 and RNA2 constructs did not produce a detectable infection but, again, inclusion of a construct encoding the CP led to high levels of infection. Thus, RBDV replication is greatly stimulated by the presence of the CP, a mechanism that also operates with ilarviruses and alfalfa mosaic virus, where it is referred to as genome activation. Mutation to remove amino acids from the N terminus of the CP showed that the first 15 RBDV CP residues are not required for genome activation. Other experiments, in which overlapping regions at the CP N terminus were fused to the monomeric red fluorescent protein, showed that sequences downstream of the first 48 aa are not absolutely required for genome activation.

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2009-03-01
2024-11-14
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References

  1. Barbara D. J., Jones A. T., Henderson S. J., Wilson S. C., Knight V. H. 1984; Isolates of raspberry bushy dwarf virus differing in Rubus host range. Ann Appl Biol 105:49–54 [CrossRef]
    [Google Scholar]
  2. Barnett O. W., Murant A. F. 1970; Host range, properties and purification of raspberry bushy dwarf virus. Ann Appl Biol 65:435–449 [CrossRef]
    [Google Scholar]
  3. Bol J. F. 2005; Replication of Alfamo- and Ilarviruses: role of the coat protein. Annu Rev Phytopathol 43:39–62 [CrossRef]
    [Google Scholar]
  4. Bol J. F., van Vloten-Doting L., Jaspars E. M. J. 1971; A functional equivalence of top component α RNA and coat protein in the initiation of infection by alfalfa mosaic virus. Virology 46:73–85 [CrossRef]
    [Google Scholar]
  5. Curtis M. D., Grossniklaus U. 2003; A gateway cloning vector set for high-throughput functional analysis of genes in planta. Plant Physiol 133:462–469 [CrossRef]
    [Google Scholar]
  6. Houwing C. J., Jaspars E. M. J. 2000; Activation of the alfalfa mosaic virus genome by viral coat protein in non-transgenic plants and protoplasts. The protection model biochemically tested. Arch Virol 145:13–35 [CrossRef]
    [Google Scholar]
  7. Jones A. T. 2005; Idaeovirus. In Virus Taxonomy: Eighth Report of the International Committee on Taxonomy of Viruses pp 1063–1065Edited by Fauquet C. M., Mayo M. A., Maniloff J., Desselberger U., A L. Ball. Elsevier Academic Press;
    [Google Scholar]
  8. Jones A. T., Murant A. F., Jennings D. L. 1982; Association of raspberry bushy dwarf virus with raspberry yellows disease: reaction of Rubus species and cultivars, and the inheritance of resistance. Ann Appl Biol 100:135–147 [CrossRef]
    [Google Scholar]
  9. Jones A. T., Mayo M. A., Murant A. F. 1996; Raspberry Bushy Dwarf Idaeovirus. In The Plant Viruses, Polyhedral Virions and Bipartite Viral Genomes vol. 5 pp 283–301Edited by Harrison B. D., Murant A. F. New York: Plenum Press;
    [Google Scholar]
  10. Jones A. T., McGavin W. J., Mayo M. A., Angel-Diaz J. E., Karenlampi S. O., Kokko H. 2000; Comparisons of some properties of two laboratory variants of Raspberry Bushy Dwarf virus (RBDV) with those of three previously characterised RBDV isolates. Eur J Plant Pathol 106:623–632 [CrossRef]
    [Google Scholar]
  11. Jones A. T., McGavin W. J., Gepp V., Zimmerman M. T., Scott S. W. 2006; Purification and properties of blackberry chlorotic ringspot, a new virus species in subgroup 1 of the genus Ilarvirus found naturally infecting blackberry in the UK. Ann Appl Biol 149:125–136 [CrossRef]
    [Google Scholar]
  12. MacFarlane S. A., Wallis C. V., Taylor S. C., Goulden M. G., Wood K. R., Davies J. W. 1991; Construction and analysis of infectious transcripts synthesised from full-length cDNA clones of both genomic RNAs of pea early browning virus. Virology 182:124–129 [CrossRef]
    [Google Scholar]
  13. Mavrič I., Viršček Marn M., Koron D. 2003; First report of Raspberry bushy dwarf virus on red raspberry and grapevine in Slovenia. Plant Dis 87:1148
    [Google Scholar]
  14. Murant A. F., Chambers J., Jones A. T. 1974; Spread of raspberry bushy dwarf virus by pollination, its association with crumbly fruit, and problems of control. Ann Appl Biol 77:271–281 [CrossRef]
    [Google Scholar]
  15. Neeleman L., van der Vossen E. A. G., Bol J. F. 1993; Infection of tobacco with alfalfa mosaic virus cDNAs sheds light on the early function of the coat protein. Virology 196:883–887 [CrossRef]
    [Google Scholar]
  16. Neeleman L., Olsthoorn R. C. L., Linthorst H. J. M., Bol J. F. 2001; Translation of a non-polyadenylated viral RNA is enhanced by binding of viral coat protein or polyadenylation of the RNA. Proc Natl Acad Sci U S A 98:14286–14291 [CrossRef]
    [Google Scholar]
  17. Uhrig J. F., Canto T., Marshall D., MacFarlane S. A. 2004; Relocalization of nuclear ALY proteins to the cytoplasm by the tomato bushy stunt virus P19 pathogenicity protein. Plant Physiol 135:2411–2423 [CrossRef]
    [Google Scholar]
  18. Voinnet O., Rivas S., Mestre P., Baulcombe D. 2003; An enhanced transient expression system in plants based on suppression of gene silencing by the p19 protein of tomato bushy stunt virus. Plant J 33:949–956 [CrossRef]
    [Google Scholar]
  19. Zuidema D., Bierhuizen M. F. A., Jaspars E. M. J. 1983; Removal of the N-terminal part of the alfalfa mosaic virus coat protein interferes with the specific binding to RNA1 and genome activation. Virology 129:255–260 [CrossRef]
    [Google Scholar]
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