1887

Abstract

The GIF protein of orf virus (ORFV) binds and inhibits the ovine cytokines granulocyte–macrophage colony-stimulating factor (GM-CSF) and interleukin-2 (IL-2). An equivalent protein has so far not been found in any of the other poxvirus genera and we therefore investigated whether it was conserved in the parapoxviruses. The corresponding genes from both the bovine-specific pseudocowpox virus (PCPV) and bovine papular stomatitis virus (BPSV) were cloned and sequenced. The predicted amino acid sequences of the PCPV and BPSV proteins shared 88 and 37 % identity, respectively, with the ORFV protein. Both retained the six cysteine residues and the WSXWS-like motif that are required for biological activity of the ORFV protein. However, an analysis of the biological activity of the two recombinant proteins revealed that, whilst the PCPV GIF protein bound to both ovine and bovine GM-CSF and IL-2 with very similar binding affinities to the ORFV GIF protein, no GM-CSF- or IL-2-binding activity was found for the BPSV protein.

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2009-04-01
2019-11-22
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vol. , part 4, pp. 970 – 977

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A range of concentrations of an affinity-purified IgG fraction from a rabbit anti-GIF serum (horizontally hatched bars) was incubated with partially purified rGIF-BPSV pre-bound to 96-well plates. Specific binding was demonstrated by a reduced level of binding when the antiserum was pre-incubated with conditioned medium containing 20–50 µg ml of rGIF PCPV (shaded bars) or rGIF ORFV (filled bars). No significant reduction in binding was observed when the antiserum was pre-incubated with medium taken from untransfected CHO cells (empty bars). * <0.001 compared with the rGIF-ORFV control.

IMAGE

Northern blots of RNA isolated from PCPV-infected (lane 3) and BPSV-infected (lane 1, V660; lane 2, A599) cells 24 h post-infection. Panel (a) was probed with P-labelled PCPV GIF cDNA, whilst panel (b) was probed with P-labelled BPSV (V660) GIF cDNA. A smear representative of poxvirus late mRNA transcripts is visible in lane 3, panel (a), and lanes 1 and 2, panel (b).

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