1887

Abstract

During the 1980s, bovine spongiform encephalopathy (BSE)-contaminated meat and bonemeal were probably fed to sheep, raising concerns that BSE may have been transmitted to sheep in the UK. The human disease, variant Creutzfeldt–Jakob disease, arose during the BSE epidemic, and oral exposure of humans to BSE-infected tissues has been implicated in its aetiology. The concern is that sheep BSE could provide another source of BSE exposure to humans via sheep products. Two immunological techniques, Western immunoblotting (WB) and immunohistochemistry (IHC), have been developed to distinguish scrapie from cases of experimental sheep BSE by the characteristics of their respective abnormal, disease-associated prion proteins (PrP). This study compares the WB and IHC characteristics of PrP from brains of primary, secondary and tertiary experimental ovine BSE cases with those of cattle BSE and natural sheep scrapie. Discrimination between experimental sheep BSE and scrapie remained possible by both methods, regardless of the route of challenge.

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2009-03-01
2019-11-17
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References

  1. Baylis, M., Houston, F., Kao, R. R., McLean, A. R., Hunter, N. & Gravenor, M. B. ( 2002; ). BSE – a wolf in sheep's clothing? Trends Microbiol 10, 563–570.[CrossRef]
    [Google Scholar]
  2. Bellworthy, S. J., Dexter, G., Stack, M., Chaplin, M., Hawkins, S. A. C. & Simmons, M. M. ( 2005; ). Natural transmission of BSE in sheep. Vet Rec 157, 206 [CrossRef]
    [Google Scholar]
  3. Bruce, M. E. & Dickinson, A. G. ( 1987; ). Biological evidence that scrapie agent has an independent genome. J Gen Virol 68, 79–89.[CrossRef]
    [Google Scholar]
  4. Bruce, M. E., Will, R. G., Ironside, J. W., McConnell, I., Drummond, D., Suttie, A, McCardle, L., Chree, A., Hope, J. & other authors ( 1997; ). Transmissions to mice indicate that ‘new variant’ CJD is caused by the BSE agent. Nature 389, 498–501.[CrossRef]
    [Google Scholar]
  5. Eloit, M., Adjou, K., Coulpier, M., Fontaine, J. J., Hamel, R., Lilin, T., Messiaen, S., Andreoletti, O., Baron, T. & other authors ( 2005; ). BSE agent signatures in a goat. Vet Rec 156, 523–524.
    [Google Scholar]
  6. Foster, J. D., Hope, J. & Fraser, H. ( 1993; ). Transmission of bovine spongiform encephalopathy to sheep and goats. Vet Rec 133, 339–341.[CrossRef]
    [Google Scholar]
  7. González, L., Martin, S., Begara-McGorum, I., Hunter, N., Houston, F., Simmons, M. & Jeffrey, M. ( 2002; ). Effects of agent strain and host genotype on PrP accumulation in the brain of sheep naturally and experimentally affected with scrapie. J Comp Pathol 126, 17–29.[CrossRef]
    [Google Scholar]
  8. González, L., Martin, S., Houston, F. E., Hunter, N., Reid, H. W., Bellworthy, S. J. & Jeffrey, M. ( 2005; ). Phenotype of disease-associated PrP accumulation in the brain of bovine spongiform encephalopathy experimentally infected sheep. J Gen Virol 86, 827–838.[CrossRef]
    [Google Scholar]
  9. Hill, A. F., Sidle, K. C. L., Joiner, S., Keyes, P., Martin, T. C., Dawson, M. & Collinge, J. ( 1998; ). Molecular screening of sheep for bovine spongiform encephalopathy. Neurosci Lett 255, 159–162.[CrossRef]
    [Google Scholar]
  10. Hope, J., Wood, S. C. E. R., Birkett, C. R., Chong, A., Bruce, M. E., Cairns, D., Goldmann, W., Hunter, N. & Bostock, C. J. ( 1999; ). Molecular analysis of ovine prion protein identifies similarities between BSE and an experimental isolate of natural scrapie, CH1641. J Gen Virol 80, 1–4.
    [Google Scholar]
  11. Hunter, N., Foster, J., Chong, A., McCutcheon, S., Parnham, D., Eaton, S., MacKenzie, C. & Houston, F. ( 2002; ). Transmission of prion diseases by blood transfusion. J Gen Virol 83, 2897–2905.
    [Google Scholar]
  12. Jeffrey, M., Martin, S., Gonzalez, L., Ryder, S. J., Bellworthy, S. J. & Jackman, R. ( 2001a; ). Differential diagnosis of infections with the bovine spongiform encephalopathy (BSE) and scrapie agents in sheep. J Comp Pathol 125, 271–284.[CrossRef]
    [Google Scholar]
  13. Jeffrey, M., Ryder, S., Martin, S., Hawkins, S. A. C., Terry, L., Berthelin-Baker, C. & Bellworthy, S. J. ( 2001b; ). Oral inoculation of sheep with the agent of bovine spongiform encephalopathy (BSE). 1: Onset and distribution of disease specific PrP accumulation in brain and viscera. J Comp Pathol 124, 280–289.[CrossRef]
    [Google Scholar]
  14. Legname, G., Baskakov, I. V., Nguyen, H. O., Riesner, D., Cohen, F. E., DeArmond, S. J. & Prusiner, S. B. ( 2004; ). Synthetic mammalian prions. Science 305, 673–676.[CrossRef]
    [Google Scholar]
  15. Martin, S., González, L., Chong, A., Houston, F., Hunter, N. & Jeffrey, M. ( 2005; ). Immunohistochemical characteristics of disease-associated PrP are not altered by host genotype or route of inoculation following infection of sheep with bovine spongiform encephalopathy. J Gen Virol 86, 839–848.[CrossRef]
    [Google Scholar]
  16. Nonno, R., Esposito, E., Vaccari, G., Conte, M., Marcon, S., Di Bari, M., Ligios, C., Di Guardo, G. & Agrimi, U. ( 2003; ). Molecular analysis of cases of Italian sheep scrapie and comparison with cases of bovine spongiform encephalopathy (BSE) and experimental BSE in sheep. J Clin Microbiol 41, 4127–4133.[CrossRef]
    [Google Scholar]
  17. Prusiner, S. B. ( 1982; ). Novel proteinaceous infectious particles cause scrapie. Science 216, 136–144.[CrossRef]
    [Google Scholar]
  18. Sisó, S., González, L., Houston, F., Hunter, N., Martin, S. & Jeffrey, M. ( 2006; ). The neuropathologic phenotype of experimental ovine BSE is maintained after blood transfusion. Blood 108, 745–748.[CrossRef]
    [Google Scholar]
  19. Stack, M. J., Chaplin, M. J. & Clark, J. ( 2002; ). Differentiation of prion protein glycoforms from naturally occurring sheep scrapie, sheep-passaged scrapie strains (CH1641 and SSBP1). bovine spongiform encephalopathy (BSE) cases and Romney and Cheviot breed sheep experimentally inoculated with BSE using two monoclonal antibodies. Acta Neuropathol 104, 279–286.
    [Google Scholar]
  20. Stack, M. J., Jeffrey, M., Gubbins, S., Grimmer, S., González, L., Martin, S., Chaplin, M., Webb, P., Simmons, M. & other authors ( 2006; ). Monitoring for BSE in sheep in Great Britain, 1998–2004. J Gen Virol 87, 2099–2107.[CrossRef]
    [Google Scholar]
  21. Wilesmith, J. W., Ryan, J. B. M. & Atkinson, M. J. ( 1991; ). Bovine spongiform encephalopathy; epidemiological studies on the origin. Vet Rec 128, 199–203.[CrossRef]
    [Google Scholar]
  22. Will, R. G., Ironside, J. W., Zeidler, M., Cousens, S. N., Estibeiro, K., Alperovitch, A., Poser, S., Pocchiari, M., Hofman, A. & Smith, P. G. ( 1996; ). A new variant of Creutzfeldt–Jakob disease in the UK. Lancet 347, 921–925.[CrossRef]
    [Google Scholar]
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