1887

Abstract

Deformed wing virus (DWV) normally causes covert infections but can have devastating effects on bees by inducing morphological deformity or even death when transmitted by the ectoparasitic mite . In order to determine the role of in the development of crippled wings, we analysed individual mites for the presence and replication of DWV. The results supported the correlation between viral replication in mites and morphologically deformed bees. Quantification of viral genome equivalents revealed that mites capable of inducing an overt DWV infection contained 10–10 genome equivalents per mite. In contrast, mites which could not induce crippled wings contained a maximum of only 10 viral genome equivalents per mite. We conclude that the development of crippled wings not only depends on DWV transmission by but also on viral replication in and on the DWV titre in the parasitizing mites.

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2009-02-01
2019-11-21
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References

  1. Ball, B. V. ( 1983; ). The association of Varroa jacobsoni with virus diseases of honey bees. In Proceedings of a Meeting of the EC Experts’ Group, Wageningen, 7–9 February 1983. Rotterdam: A. A. Balkema.
  2. Ball, B. V. ( 1985; ). Acute paralysis virus isolates from honeybee, Apis mellifera, colonies infested with Varroa jacobsoni. J Apic Res 24, 115–119.
    [Google Scholar]
  3. Ball, B. V. & Allen, M. E. ( 1988; ). The prevalence of pathogens in honey bee (Apis mellifera) colonies infested with the parasitic mite Varroa jacobsoni. Ann Appl Biol 113, 237–244.[CrossRef]
    [Google Scholar]
  4. Bowen-Walker, P. L., Martin, S. J. & Gunn, A. ( 1999; ). The transmission of deformed wing virus between honeybees (Apis mellifera L.) by the ectoparasitic mite Varroa jacobsoni Oud. J Invertebr Pathol 73, 101–106.[CrossRef]
    [Google Scholar]
  5. Celle, O., Blanchard, P., Olivier, V., Schurr, F., Cougoule, N., Faucon, J.-P. & Ribiere, M. ( 2008; ). Detection of Chronic bee paralysis virus (CBPV) genome and its replicative RNA form in various hosts and possible ways of spread. Virus Res 133, 280–284.[CrossRef]
    [Google Scholar]
  6. Chen, Y. P., Higgins, J. A. & Feldlaufer, M. F. ( 2005; ). Quantitative real-time reverse transcription-PCR analysis of Deformed wing virus infection in the honeybee (Apis mellifera L.). Appl Environ Microbiol 71, 436–441.[CrossRef]
    [Google Scholar]
  7. Cox-Foster, D. L., Conlan, S., Holmes, E. C., Palacios, G., Evans, J. D., Moran, N. A., Quan, P.-L., Briese, S., Hornig, M. & other authors ( 2007; ). A metagenomic survey of microbes in honey bee colony collapse disorder. Science 318, 283–287.[CrossRef]
    [Google Scholar]
  8. Craggs, J. K., Ball, J. K., Thomson, B. J., Irving, W. L. & Grabowska, A. M. ( 2001; ). Development of a strand-specific RT-PCR based assay to detect the replicative form of hepatitis C virus RNA. J Virol Methods 94, 111–120.[CrossRef]
    [Google Scholar]
  9. Domingo, E. ( 2002; ). Quasispecies theory in virology. J Virol 76, 463–465.[CrossRef]
    [Google Scholar]
  10. Eigen, M. ( 1996; ). On the nature of virus quasispecies. Trends Microbiol 4, 216–218.[CrossRef]
    [Google Scholar]
  11. Genersch, E. ( 2005; ). Development of a rapid and sensitive RT-PCR method for the detection of deformed wing virus, a pathogen of the honeybee (Apis mellifera). Vet J 169, 121–123.[CrossRef]
    [Google Scholar]
  12. Holmes, E. C. & Moya, A. ( 2002; ). Is the quasispecies concept relevant to RNA viruses? J Virol 76, 460–462.[CrossRef]
    [Google Scholar]
  13. Hung, A. C. F., Adams, J. R. & Shimanuki, H. ( 1995; ). Bee parasitic mite syndrome (II): the role of Varroa mite and viruses. Am Bee J 135, 702
    [Google Scholar]
  14. Hung, A. C., Shimanuki, H. & Knox, D. V. ( 1996; ). The role of viruses in bee parasitic mite syndrome. Am Bee J 136, 731–732.
    [Google Scholar]
  15. Lanzi, G., De Miranda, J. R., Boniotti, M. B., Cameron, C. E., Lavazza, A., Capucci, L., Camazine, S. M. & Rossi, C. ( 2006; ). Molecular and biological characterization of Deformed wing virus of honeybees (Apis mellifera L.). J Virol 80, 4998–5009.[CrossRef]
    [Google Scholar]
  16. Laskus, T., Radkowski, M., Wang, L.-F., Vargas, H. & Rakela, J. ( 1998; ). Detection of hepatitis G virus replication sites by using highly strand-specific Tth-based reverse transcriptase PCR. J Virol 72, 3072–3075.
    [Google Scholar]
  17. Martin, S. J. ( 2001; ). The role of Varroa and viral pathogens in the collapse of honeybee colonies: a modelling approach. J Appl Ecol 38, 1082–1093.
    [Google Scholar]
  18. Martin, S. J., Hogarth, A., van Breda, J. & Perrett, J. ( 1998; ). A scientific note on Varroa jacobsoni Oudemans and the collapse of Apis mellifera L. colonies in the United Kingdom. Apidologie (Celle) 29, 369–370.[CrossRef]
    [Google Scholar]
  19. Ongus, J. R., Peters, D., Bonmatin, J.-M., Bengsch, E., Vlak, J. M. & van Oers, M. M. ( 2004; ). Complete sequence of a picorna-like virus of the genus Iflavirus replicating in the mite Varroa destructor. J Gen Virol 85, 3747–3755.[CrossRef]
    [Google Scholar]
  20. Shen, M., Cui, L., Ostiguy, N. & Cox-Foster, D. ( 2005a; ). Intricate transmission routes and interaction between picorna-like viruses (Kashmir bee virus and sacbrood virus) with the honey bee host and the parasitic varroa mite. J Gen Virol 86, 2281–2289.[CrossRef]
    [Google Scholar]
  21. Shen, M., Yang, X., Cox-Foster, D. & Cui, L. ( 2005b; ). The role of varroa mites in infections of Kashmir bee virus (KBV) and deformed wing virus (DWV) in honey bees. Virology 342, 141–149.[CrossRef]
    [Google Scholar]
  22. Shimanuki, H., Calderone, N. W. & Knox, D. A. ( 1994; ). Parasitic mite syndrome: the symptoms. Am Bee J 134, 827–828.
    [Google Scholar]
  23. Sumpter, D. J. T. & Martin, S. J. ( 2004; ). The dynamics of virus epidemics in Varroa-infested honey bee colonies. J Anim Ecol 73, 51–63.[CrossRef]
    [Google Scholar]
  24. Tentcheva, D., Gauthier, L., Bagny, L., Fievet, J., Dainat, B., Cousserans, F., Colin, M. E. & Bergoin, M. ( 2006; ). Comparative analysis of deformed wing virus (DWV) RNA in Apis mellifera and Varroa destructor. Apidologie (Celle) 37, 41–50.[CrossRef]
    [Google Scholar]
  25. Yang, X. ( 2004; ). Effects of varroa mites on the immune system and pathology of honey bees. PhD thesis. The Pennsylvania State University, University Park, Pennsylvania, USA.
  26. Yang, X. & Cox-Foster, D. L. ( 2005; ). Impact of an ectoparasite on the immunity and pathology of an invertebrate: evidence for host immunosuppression and viral amplification. Proc Natl Acad Sci U S A 102, 7470–7475.[CrossRef]
    [Google Scholar]
  27. Yang, X. & Cox-Foster, D. ( 2007; ). Effects of parasitization by Varroa destructor on survivorship and physiological traits of Apis mellifera in correlation with viral incidence and microbial challenge. Parasitology 134, 405–412.[CrossRef]
    [Google Scholar]
  28. Yue, C. & Genersch, E. ( 2005; ). RT-PCR analysis of Deformed wing virus in honeybees (Apis mellifera) and mites (Varroa destructor). J Gen Virol 86, 3419–3424.[CrossRef]
    [Google Scholar]
  29. Yue, C., Schröder, M., Gisder, S. & Genersch, E. ( 2007; ). Vertical transmission routes for Deformed wing virus of honeybees (Apis mellifera). J Gen Virol 88, 2329–2336.[CrossRef]
    [Google Scholar]
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