Identification of sequence elements regulating promoter activity and replication of a monopartite begomovirus-associated DNA satellite Free

Abstract

DNA is a circular single-stranded satellite DNA associated with certain monopartite begomoviruses (family ) which causes economically important diseases such as cotton leaf curl disease. DNA contains a single gene, , which encodes a pathogenicity protein responsible for symptom production. Transient expression studies in using the -glucuronidase reporter gene driven by a promoter-deletion series of the DNA associated with cotton leaf curl Multan virus identified a 68 nt region (between −139 and −207) which is important for transcription. This 68 nt region contains a G-box (CACGTG) located 143 nt upstream of the start codon. Mutation of the G-box resulted in a significant reduction in promoter activity and DNA replication efficiency. In addition, the G-box motif was found to bind specifically to a protein(s) in nuclear extracts prepared from tobacco leaf tissues. Our results indicate that interaction of the G-box motif with host nuclear factors is important for efficient gene expression and replication of DNA .

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2009-01-01
2024-03-29
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References

  1. Arguello-Astorga, G. R., Guevara-Gonzalez, R. G., Herrera-Estrella, L. R. & Rivera-Bustamante, R. F.(1994). Geminivirus replication origins have a group-specific organization of iterative elements: a model for replication. Virology 203, 90–100.[CrossRef] [Google Scholar]
  2. Armentero, M. T., Horwitz, M. & Mermod, N.(1994). Targeting of DNA polymerase to the adenovirus origin of DNA replication by interaction with nuclear factor I. Proc Natl Acad Sci U S A 91, 11537–11541.[CrossRef] [Google Scholar]
  3. Briddon, R. W. & Stanley, J.(2006). Subviral agents associated with plant single-stranded DNA viruses. Virology 344, 198–210.[CrossRef] [Google Scholar]
  4. Briddon, R. W., Mansoor, S., Bedford, I. D., Pinner, M. S., Saunders, K., Stanley, J., Zafar, Y., Malik, K. A. & Markham, P. G.(2001). Identification of DNA components required for induction of cotton leaf curl disease. Virology 285, 234–243.[CrossRef] [Google Scholar]
  5. Briddon, R. W., Bull, S. E., Amin, I., Idris, A. M., Mansoor, S., Bedford, I. D., Dhawan, P., Rishi, N., Siwatch, S. S. & other authors(2003). Diversity of DNA β, a satellite molecule associated with some monopartite begomoviruses. Virology 312, 106–121.[CrossRef] [Google Scholar]
  6. Brown, J. K.(1992). Whitefly-transmitted geminiviruses and associated disorders in the Americas and the Caribbean basin. Plant Dis 7, 220–225. [Google Scholar]
  7. Chen, P. Y., Wang, C. K., Soong, S. C. & To, K. Y.(2003). Complete sequence of the binary vector pBI121 and its application in cloning T-DNA insertion from transgenic plants. Mol Breed 11, 287–293.[CrossRef] [Google Scholar]
  8. Davidson, B. P., Dogra, S. C. & May, B. K.(2001). A duplicated HNF-3 binding site in the CYP2H2 promoter underlies the weak phenobarbital induction response. Int J Biochem Cell Biol 33, 1080–1093.[CrossRef] [Google Scholar]
  9. DePamphilis, M. L.(1993). How transcription factors regulate origins of DNA replication in eukaryotic cells. Trends Cell Biol 3, 161–167.[CrossRef] [Google Scholar]
  10. Donald, R. G. & Cashmore, A. R.(1990). Mutation of either G box or I box sequences profoundly affects expression from the ArabidopsisrbcS-1A promoter. EMBO J 9, 1717–1726. [Google Scholar]
  11. Dry, I. B., Krake, L. R., Rigden, J. E. & Rezaian, M. A.(1997). A novel subviral agent associated with a geminivirus: the first report of a DNA satellite. Proc Natl Acad Sci U S A 94, 7088–7093.[CrossRef] [Google Scholar]
  12. Eagle, P. A. & Hanley-Bowdoin, L.(1997).Cis elements that contribute to geminivirus transcriptional regulation and the efficiency of DNA replication. J Virol 71, 6947–6955. [Google Scholar]
  13. Eagle, P. A., Orozco, B. M. & Hanley-Bowdoin, L.(1994). A DNA sequence required for geminivirus replication also mediates transcriptional regulation. Plant Cell 6, 1157–1170.[CrossRef] [Google Scholar]
  14. Escobar, C., Aristizéabal, F., Navas, A., Del Campo, F. F. & Fenoll, C.(2001). Isolation of active DNA-binding nuclear proteins from tomato galls induced by root-knot nematodes. Plant Mol Biol Rep 19, 375–376.[CrossRef] [Google Scholar]
  15. Fenoll, C., Schwarz, J. J., Black, D. M., Schneider, M. & Howell, S. H.(1990). The intergenic region of Maize streak virus contains a GC-rich element that activates rightward transcription and binds maize nuclear factors. Plant Mol Biol 15, 865–877.[CrossRef] [Google Scholar]
  16. Guan, C. & Zhou, X.(2006). Phloem specific promoter from a satellite associated with a DNA virus. Virus Res 115, 150–157.[CrossRef] [Google Scholar]
  17. Haley, A., Zhan, X., Richardson, K., Head, K. & Morris, B.(1992). Regulation of the activities of African cassava mosaic virus promoters by the AC1, AC2, and AC3 gene products. Virology 188, 905–909.[CrossRef] [Google Scholar]
  18. Hanley-Bowdoin, L., Settlage, S. B., Orozco, B. M., Nagar, S. & Robertson, D.(1999). Geminiviruses: models for plant DNA replication, transcription, and cell cycle regulation. Crit Rev Biochem Mol Biol 35, 105–140. [Google Scholar]
  19. Harrison, B. D. & Robinson, D. J.(2002). Green shoots of geminivirology. Physiol Mol Plant Pathol 60, 215–218.[CrossRef] [Google Scholar]
  20. Hennegan, K. P. & Danna, K. J.(1998). pBIN20: an improved binary vector for Agrobacterium-mediated transformation. Plant Mol Biol Rep 16, 129–131.[CrossRef] [Google Scholar]
  21. Jefferson, R. A., Kavanagh, T. A. & Bevan, M. W.(1987). GUS fusions: β-glucuronidase as a sensitive and versatile gene fusion marker in higher plants. EMBO J 6, 3901–3907. [Google Scholar]
  22. Jose, J. & Ramakrishnan, U.(2003). Bhendi yellow vein mosaic disease in India is caused by association of a DNA β satellite with a begomovirus. Virology 305, 310–317.[CrossRef] [Google Scholar]
  23. Khan, S. M., Jiang, W., Borner, C., O'Driscoll, K. & Weinstein, I. B.(1990). Construction of defined deletion mutants by thermal cycled fusion: applications to protein kinase C. Technique (PHILA) 2, 27–30. [Google Scholar]
  24. Lam, E., Benfey, P. N., Gilmartin, P. M., Fang, R.-X. & Chua, N.-H.(1989). Site-specific mutations alter in vitro factor binding and change promoter expression pattern in transgenic plants. Proc Natl Acad Sci U S A 86, 7890–7894.[CrossRef] [Google Scholar]
  25. Lescot, M., Dehais, P., Thijs, G., Marchal, K., Moreau, Y., Van de Peer, Y., Rouze, P. & Rombauts, S.(2002). PlantCARE, a database of plant cis-acting regulatory elements and a portal to tools for in silico analysis of promoter sequences. Nucleic Acids Res 30, 325–327.[CrossRef] [Google Scholar]
  26. Lue, N. F. & Kornberg, R. D.(1993). A possible role for the yeast TATA element-binding protein in DNA replication. Proc Natl Acad Sci U S A 90, 8018–8022.[CrossRef] [Google Scholar]
  27. Mansoor, S., Briddon, W., Bull, S. E., Bedford, I. D., Bashir, A., Hussain, M., Saeed, M., Zafar, Y., Malik, K. A. & other authors(2003). Cotton leaf curl disease is associated with multiple monopartite begomoviruses supported by single DNA β. Arch Virol 148, 1969–1986.[CrossRef] [Google Scholar]
  28. McKendree, W. L. & Ferl, R. J.(1992). Functional elements of the ArabidopsisAdh promoter include the G-box. Plant Mol Biol 19, 859–862.[CrossRef] [Google Scholar]
  29. Moriones, E. & Navas-Castillo, J.(2000).Tomato yellow leaf curl virus, an emerging virus complex causing epidemics worldwide. Virus Res 71, 123–134.[CrossRef] [Google Scholar]
  30. Polston, J. E., Bois, D., Serra, C. A. & Concepcion, S.(1994). First report of a tomato yellow leaf curl-like geminivirus in the western hemisphere. Plant Dis 78, 831 [Google Scholar]
  31. Rigden, J. E., Dry, I. B., Krake, L. R. & Rezaian, M. A.(1996). Plant virus DNA replication processes in Agrobacterium: insight into the origins of geminiviruses? Proc Natl Acad Sci U S A 93, 10280–10284.[CrossRef] [Google Scholar]
  32. Ruiz-Medrano, R., Guevara-Gonzalez, R. G., Arguello-Astorga, G. R., Monsalve-Fonnegra, Z., Herrera-Estrella, L. R. & Rivera-Bustamante, R. F.(1999). Identification of a sequence element involved in AC2-mediated transactivation of the Pepper huasteco virus coat protein gene. Virology 253, 162–169.[CrossRef] [Google Scholar]
  33. Saeed, M., Behjatnia, S. A. A., Shahid, M., Yusuf, Z., Shahida, H. & Rezaian, M. A.(2005). A single complementary-sense transcript of a geminiviral DNA β satellite is determinant of pathogenicity. Mol Plant Microbe Interact 18, 7–14.[CrossRef] [Google Scholar]
  34. Saunders, K., Bedford, I. D., Briddon, R. W., Markham, P. G., Wong, S. M. & Stanley, J.(2000). A unique virus complex causes Ageratum yellow vein disease. Proc Natl Acad Sci U S A 97, 6890–6895.[CrossRef] [Google Scholar]
  35. Schindler, U., Beckmann, H. & Cashmore, A. R.(1992). TGA1 and G-box binding factors: two distinct classes of Arabidopsis leucine zipper proteins compete for the G-box-like element TGACGTGG. Plant Cell 4, 1309–1319.[CrossRef] [Google Scholar]
  36. Shivaprasad, P. V., Akbergenov, R., Trinks, D., Rajeswaran, R., Veluthambi, K., Hohn, T. & Pooggin, M. M.(2005). Promoters, transcripts, and regulatory proteins of Mungbean yellow mosaic geminivirus. J Virol 79, 8149–8163.[CrossRef] [Google Scholar]
  37. Shung, C. Y., Sunter, J., Sirasanagandla, S. S. & Sunter, G.(2006). Distinct viral sequence elements are necessary for expression of Tomato golden mosaic virus complementary sense transcripts. Mol Plant Microbe Interact 19, 1394–1405.[CrossRef] [Google Scholar]
  38. Sunter, G. & Bisaro, D. M.(1991). Transactivation in a geminivirus: AL2 gene product is needed for coat protein expression. Virology 180, 416–419.[CrossRef] [Google Scholar]
  39. Sunter, G. & Bisaro, D. M.(1992). Transactivation of geminivirus AR1 and BR1 gene expression by the viral AL2 gene product occurs at the level of transcription. Plant Cell 4, 1321–1331.[CrossRef] [Google Scholar]
  40. Sunter, G. & Bisaro, D. M.(2003). Identification of a minimal sequence required for activation of the Tomato golden mosaic virus coat protein promoter in protoplasts. Virology 305, 452–462.[CrossRef] [Google Scholar]
  41. Takos, A. M., Ubi, B. E., Robinson, S. P. & Walker, A. R.(2006). Condensed tannin biosynthesis genes are regulated separately from other flavonoid biosynthesis genes in apple fruit skin. Plant Sci 170, 487–499.[CrossRef] [Google Scholar]
  42. Tu, J. & Sunter, G.(2007). A conserved binding site within the Tomato golden mosaic virusAL-1629 promoter is necessary for expression of viral genes important for pathogenesis. Virology 367, 117–125.[CrossRef] [Google Scholar]
  43. Usharani, K. S., Periasamy, M. & Malathi, V. G.(2006). Studies on the activity of a bidirectional promoter of Mungbean yellow mosaic India virus by agroinfiltration. Virus Res 119, 154–162.[CrossRef] [Google Scholar]
  44. Xie, Y., Liu, Y., Meng, M., Chen, L. & Zhu, Z.(2003). Isolation and identification of a super strong plant promoter from Cotton leaf curl Multan virus. Plant Mol Biol 53, 1–14.[CrossRef] [Google Scholar]
  45. Yang, Y., Li, R. & Qi, M.(2000).In vivo analysis of plant promoters and transcription factors by agroinfiltration of tobacco leaves. Plant J 22, 543–551.[CrossRef] [Google Scholar]
  46. Yin, Y., Chen, L. & Beachy, R.(1997). Promoter elements required for phloem-specific gene expression from the RTBV promoter in rice. Plant J 12, 1179–1188.[CrossRef] [Google Scholar]
  47. Zhan, X. C., Haley, A., Richardson, K. & Morris, B.(1991). Analysis of the potential promoter sequences of African cassava mosaic virus by transient expression of the β-glucuronidase gene. J Gen Virol 72, 2849–2852.[CrossRef] [Google Scholar]
  48. Zhou, X., Xie, Y., Tao, X., Zhang, Z., Li, Z. & Fauquet, C. M.(2003). Characterization of DNA β associated with begomoviruses in China and evidence for co-evolution with their cognate viral DNA-A. J Gen Virol 84, 237–247.[CrossRef] [Google Scholar]
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