DNA is a circular single-stranded satellite DNA associated with certain monopartite begomoviruses (family ) which causes economically important diseases such as cotton leaf curl disease. DNA contains a single gene, , which encodes a pathogenicity protein responsible for symptom production. Transient expression studies in using the -glucuronidase reporter gene driven by a promoter-deletion series of the DNA associated with cotton leaf curl Multan virus identified a 68 nt region (between −139 and −207) which is important for transcription. This 68 nt region contains a G-box (CACGTG) located 143 nt upstream of the start codon. Mutation of the G-box resulted in a significant reduction in promoter activity and DNA replication efficiency. In addition, the G-box motif was found to bind specifically to a protein(s) in nuclear extracts prepared from tobacco leaf tissues. Our results indicate that interaction of the G-box motif with host nuclear factors is important for efficient gene expression and replication of DNA .


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