RT Journal Article SR Electronic(1) A1 Sanders, Barbara P. A1 Liu, Ying A1 Brandjes, Alies A1 van Hoek, Vladimir A1 de los Rios Oakes, Isabel A1 Lewis, John A1 Wimmer, Eckard A1 Custers, Jerome H. H. V. A1 Schuitemaker, Hanneke A1 Cello, Jeronimo A1 Edo-Matas, DianaYR 2015 T1 Brunenders: a partially attenuated historic poliovirus type I vaccine strain JF Journal of General Virology, VO 96 IS 9 SP 2614 OP 2622 DO https://doi.org/10.1099/vir.0.000197 PB Microbiology Society, SN 1465-2099, AB Brunenders, a type I poliovirus (PV) strain, was developed in 1952 by J. F. Enders and colleagues through serial in vitro passaging of the parental Brunhilde strain, and was reported to display partial neuroattenuation in monkeys. This phenotype of attenuation encouraged two vaccine manufacturers to adopt Brunenders as the type I component for their inactivated poliovirus vaccines (IPVs) in the 1950s, although today no licensed IPV vaccine contains Brunenders. Here we confirmed, in a transgenic mouse model, the report of Enders on the reduced neurovirulence of Brunenders. Although dramatically neuroattenuated relative to WT PV strains, Brunenders remains more virulent than the attenuated oral vaccine strain, Sabin 1. Importantly, the neuroattenuation of Brunenders does not affect in vitro growth kinetics and in vitro antigenicity, which were similar to those of Mahoney, the conventional type I IPV vaccine strain. We showed, by full nucleotide sequencing, that Brunhilde and Brunenders differ at 31 nucleotides, eight of which lead to amino acid changes, all located in the capsid. Upon exchanging the Brunenders capsid sequence with that of the Mahoney capsid, WT neurovirulence was regained in vivo, suggesting a role for the capsid mutations in Brunenders attenuation. To date, as polio eradication draws closer, the switch to using attenuated strains for IPV is actively being pursued. Brunenders preceded this novel strategy as a partially attenuated IPV strain, accompanied by decades of successful use in the field. Providing data on the attenuation of Brunenders may be of value in the further construction of attenuated PV strains to support the grand pursuit of the global eradication of poliomyelitis., UL https://www.microbiologyresearch.org/content/journal/jgv/10.1099/vir.0.000197