1887

Abstract

Previous studies revealed that rotavirus A (RVA) is present in not only the small intestine but also various organs. It was reported that RVA persisted in mesenteric lymph nodes (MLNs) in experimental models. However, there have been no reports focused on RVA in MLNs of animals under natural conditions. In this study, in order to investigate the persistence of the RVA genome in MLNs in cattle under natural conditions, reverse transcription-semi-nested PCR was carried out to detect RVA genomes in the MLNs from 17 calves that had been subjected to autopsy examinations. RVA genomes were detected in MLNs from 10 (˜60  %) of the 17 autopsied calves. MLNs from 170 healthy adult cattle that had been slaughtered were also examined; 15 (∼10  %) of the 170 cattle had RVA genomes in their MLNs, indicating that RNA genomes are found frequently in MLNs of cattle under natural conditions. Genetic analyses revealed that RVAs in MLNs were classified as G and/or P genotypes generally prevalent in bovines. Basically, the strains in intestinal contents were genetically identical to those in MLNs from individual cattle, suggesting that bovine RVAs have the ability to spread from the intestine to MLNs. Furthermore, amongst RVA-positive cattle, six of 10 autopsied calves and 12 of 15 healthy adult cattle were negative for the virus in the intestinal contents, indicating that bovine RVA genomes can persist in MLNs after viral clearance in the digestive tract.

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2015-09-01
2019-11-13
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References

  1. Abe M., Ito N., Morikawa S., Takasu M., Murase T., Kawashima T., Kawai Y., Kohara J., Sugiyama M.. ( 2009;). Molecular epidemiology of rotaviruses among healthy calves in Japan: isolation of a novel bovine rotavirus bearing new P and G genotypes. Virus Res 144: 250–257 [CrossRef] [PubMed].
    [Google Scholar]
  2. Blutt S.E., Kirkwood C.D., Parreño V., Warfield K.L., Ciarlet M., Estes M.K., Bok K., Bishop R.F., Conner M.E.. ( 2003;). Rotavirus antigenaemia and viraemia: a common event?. Lancet 362: 1445–1449 [CrossRef] [PubMed].
    [Google Scholar]
  3. Brown K.A., Offit P.A.. ( 1998;). Rotavirus-specific proteins are detected in murine macrophages in both intestinal and extraintestinal lymphoid tissues. Microb Pathog 24: 327–331 [CrossRef] [PubMed].
    [Google Scholar]
  4. Crawford S.E., Patel D.G., Cheng E., Berkova Z., Hyser J.M., Ciarlet M., Finegold M.J., Conner M.E., Estes M.K.. ( 2006;). Rotavirus viremia and extraintestinal viral infection in the neonatal rat model. J Virol 80: 4820–4832 [CrossRef] [PubMed].
    [Google Scholar]
  5. Dhama K., Chauhan R.S., Mahendran M., Malik S.V.. ( 2009;). Rotavirus diarrhea in bovines and other domestic animals. Vet Res Commun 33: 1–23 [CrossRef] [PubMed].
    [Google Scholar]
  6. Dharakul T., Riepenhoff-Talty M., Albini B., Ogra P.L.. ( 1988;). Distribution of rotavirus antigen in intestinal lymphoid tissues: potential role in development of the mucosal immune response to rotavirus. Clin Exp Immunol 74: 14–19 [PubMed].
    [Google Scholar]
  7. Estes M.K., Greenberg H.B.. ( 2013;). Rotaviruses. . In Fields Virology, vol. 2, 6th edn.., pp. 1347–1401. Edited by Knipe D. M., Howley P. M.. Philadelphia, PA: Lippincott Williams & Wilkins;.
    [Google Scholar]
  8. Fenaux M., Cuadras M.A., Feng N., Jaimes M., Greenberg H.B.. ( 2006;). Extraintestinal spread and replication of a homologous EC rotavirus strain and a heterologous rhesus rotavirus in BALB/c mice. J Virol 80: 5219–5232 [CrossRef] [PubMed].
    [Google Scholar]
  9. Ghosh S., Samajdar S., Sinha M., Kobayashi N., Taniguchi K., Naik T.N.. ( 2008;). Molecular characterization of rare bovine group A rotavirus G15P[11] and G15P[21] strains from eastern India: identification of simian SA11-like VP6 genes in G15P[21] strains. Virus Genes 37: 241–249 [CrossRef] [PubMed].
    [Google Scholar]
  10. Goto Y., Kurogi H., Inaba Y., Matumoto M.. ( 1986;). Sequential isolation of rotavirus from individual calves. Vet Microbiol 11: 177–184 [CrossRef] [PubMed].
    [Google Scholar]
  11. Kim H.-J., Park J.-G., Matthijnssens J., Lee J.-H., Bae Y.-C., Alfajaro M.M., Park S.-I., Kang M.-I., Cho K.-O.. ( 2011;). Intestinal and extra-intestinal pathogenicity of a bovine reassortant rotavirus in calves and piglets. Vet Microbiol 152: 291–303 [CrossRef] [PubMed].
    [Google Scholar]
  12. Lynch M., Shieh W.J., Tatti K., Gentsch J.R., Ferebee-Harris T., Jiang B., Guarner J., Bresee J.S., Greenwald M., other authors. ( 2003;). The pathology of rotavirus-associated deaths, using new molecular diagnostics. Clin Infect Dis 37: 1327–1333 [CrossRef] [PubMed].
    [Google Scholar]
  13. Martella V., Bányai K., Matthijnssens J., Buonavoglia C., Ciarlet M.. ( 2010;). Zoonotic aspects of rotaviruses. Vet Microbiol 140: 246–255 [CrossRef] [PubMed].
    [Google Scholar]
  14. Matthijnssens J., Ciarlet M., Rahman M., Attoui H., Bányai K., Estes M.K., Gentsch J.R., Iturriza-Gómara M., Kirkwood C.D., other authors. ( 2008;). Recommendations for the classification of group A rotaviruses using all 11 genomic RNA segments. Arch Virol 153: 1621–1629 [CrossRef] [PubMed].
    [Google Scholar]
  15. Matthijnssens J., Potgieter C.A., Ciarlet M., Parreño V., Martella V., Bányai K., Garaicoechea L., Palombo E.A., Novo L., other authors. ( 2009;). Are human P[14] rotavirus strains the result of interspecies transmissions from sheep or other ungulates that belong to the mammalian order Artiodactyla?. J Virol 83: 2917–2929 [CrossRef] [PubMed].
    [Google Scholar]
  16. Matthijnssens J., Ciarlet M., McDonald S.M., Attoui H., Bányai K., Brister J.R., Buesa J., Esona M.D., Estes M.K., other authors. ( 2011;). Uniformity of rotavirus strain nomenclature proposed by the Rotavirus Classification Working Group (RCWG). Arch Virol 156: 1397–1413 [CrossRef] [PubMed].
    [Google Scholar]
  17. Matthijnssens J., Otto P.H., Ciarlet M., Desselberger U., Van Ranst M., Johne R.. ( 2012;). VP6-sequence-based cutoff values as a criterion for rotavirus species demarcation. Arch Virol 157: 1177–1182 [CrossRef] [PubMed].
    [Google Scholar]
  18. Miyazaki A., Kuga K., Suzuki T., Tsunemitsu H.. ( 2012;). Analysis of the excretion dynamics and genotypic characteristics of rotavirus A during the lives of pigs raised on farms for meat production. J Clin Microbiol 50: 2009–2017 [CrossRef] [PubMed].
    [Google Scholar]
  19. Mossel E.C., Ramig R.F.. ( 2003;). A lymphatic mechanism of rotavirus extraintestinal spread in the neonatal mouse. J Virol 77: 12352–12356 [CrossRef] [PubMed].
    [Google Scholar]
  20. Mukhopadhya I., Sarkar R., Menon V.K., Babji S., Paul A., Rajendran P., Sowmyanarayanan T.V., Moses P.D., Iturriza-Gomara M., other authors. ( 2013;). Rotavirus shedding in symptomatic and asymptomatic children using reverse transcription-quantitative PCR. J Med Virol 85: 1661–1668 [CrossRef] [PubMed].
    [Google Scholar]
  21. Park J.-G.G., Kim H.-J.J., Matthijnssens J., Alfajaro M.M., Kim D.-S.S., Son K.-Y.Y., Kwon H.-J.J., Hosmillo M., Ryu E.-H.H., other authors. ( 2013;). Different virulence of porcine and porcine-like bovine rotavirus strains with genetically nearly identical genomes in piglets and calves. Vet Res 44: 88 [CrossRef] [PubMed].
    [Google Scholar]
  22. Park J.-G.G., Kim D.-S.S., Matthijnssens J., Kwon H.-J.J., Zeller M., Alfajaro M.M., Son K.-Y.Y., Hosmillo M., Ryu E.-H.H., other authors. ( 2014;). Comparison of pathogenicities and nucleotide changes between porcine and bovine reassortant rotavirus strains possessing the same genotype constellation in piglets and calves. Vet Microbiol 172: 51–62 [CrossRef] [PubMed].
    [Google Scholar]
  23. Richardson S., Grimwood K., Gorrell R., Palombo E., Barnes G., Bishop R.. ( 1998;). Extended excretion of rotavirus after severe diarrhoea in young children. Lancet 351: 1844–1848 [CrossRef] [PubMed].
    [Google Scholar]
  24. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S.. ( 2011;). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28: 2731–2739 [CrossRef] [PubMed].
    [Google Scholar]
  25. Tate J.E., Burton A.H., Boschi-Pinto C., Steele A.D., Duque J., Parashar U.D., WHO-coordinated Global Rotavirus Surveillance Network. ( 2012;). 2008 estimate of worldwide rotavirus-associated mortality in children younger than 5 years before the introduction of universal rotavirus vaccination programmes: a systematic review and meta-analysis. Lancet Infect Dis 12: 136–141 [CrossRef] [PubMed].
    [Google Scholar]
  26. Trojnar E., Sachsenröder J., Twardziok S., Reetz J., Otto P.H., Johne R.. ( 2013;). Identification of an avian group A rotavirus containing a novel VP4 gene with a close relationship to those of mammalian rotaviruses. J Gen Virol 94: 136–142 [CrossRef] [PubMed].
    [Google Scholar]
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