Ferrets have become the model animal of choice for influenza pathology and transmission experiments as they are permissive and susceptible to human influenza A viruses. However, inoculation of ferrets with mumps virus (MuV) did not lead to successful infections. We evaluated the use of highly differentiated ferret tracheal epithelium cell cultures, FTE, for predicting the potential of ferrets to support respiratory viral infections. FTE cultures supported productive replication of human influenza A and B viruses but not of MuV, whereas analogous cells generated from human airways supported replication of all three viruses. We propose that in vitro strategies using these cultures might serve as a method of triaging viruses and potentially reducing the use of ferrets in viral studies.
BelserJ.A.,
KatzJ.M.,
TumpeyT.M.2011; The ferret as a model organism to study influenza A virus infection. Dis Model Mech 4:575–579 [View Article][PubMed]
BodewesR.,
MorickD.,
de MutsertG.,
OsingaN.,
BestebroerT.,
van der VlietS.,
SmitsS.L.,
KuikenT.,
RimmelzwaanG.F.,
other authors.
2013; Recurring influenza B virus infections in seals. Emerg Infect Dis 19:511–512 [View Article][PubMed]
BossartK.N.,
ZhuZ.,
MiddletonD.,
KlippelJ.,
CrameriG.,
BinghamJ.,
McEachernJ.A.,
GreenD.,
HancockT.J.,
other authors.
2009; A neutralizing human monoclonal antibody protects against lethal disease in a new ferret model of acute nipah virus infection. PLoS Pathog 5:e1000642[CrossRef]
BrookesD.W.,
MiahS.,
LackenbyA.,
HartgrovesL.,
BarclayW.S.2010; Pandemic H1N1 2009 influenza virus with the H275Y oseltamivir resistance neuraminidase mutation shows a small compromise in enzyme activity and viral fitness. J Antimicrob Chemother
ElderfieldR.A.,
WatsonS.J.,
GodleeA.,
AdamsonW.E.,
ThompsonC.I.,
DunningJ.,
Fernandez-AlonsoM.,
BlumenkrantzD.,
HussellT.other authors2014; Accumulation of human-adapting mutations during circulation of A(H1N1)pdm09 influenza in humans in the UK. J Virol
HerlocherM.L.,
EliasS.,
TrusconR.,
HarrisonS.,
MindellD.,
SimonC.,
MontoaS.2001; Ferrets as a transmission model for influenza: sequence changes in HA1 of type A (H3N2) virus. J Infect Dis 184:542–546[CrossRef]
HuangS.S.H.,
BannerD.,
PaquetteS.G.,
LeonA.J.,
KelvinA.A.,
KelvinD.J.2014; Pathogenic influenza B virus in the ferret model establishes lower respiratory tract infection. J Gen Virol 95:2127–2139 [View Article][PubMed]
JacksonD.,
CadmanA.,
ZurcherT.,
BarclayW.S.2002; A reverse genetics approach for recovery of recombinant influenza B viruses entirely from cDNA. J Virol 76:11744–11747[CrossRef]
JiaN.,
BarclayW.S.,
RobertsK.,
YenH.-L.,
ChanR.W.Y.,
LamA.K.Y.,
AirG.,
PeirisJ.S.M.,
DellA.,
other authors.
2014; Glycomic characterization of respiratory tract tissues of ferrets: implications for its use in influenza virus infection studies. J Biol Chem 289:28489–28504 [View Article][PubMed]
KimY.H.,
KimH.S.,
ChoS.H.,
SeoS.H.2009; Influenza B virus causes milder pathogenesis and weaker inflammatory responses in ferrets than influenza A virus. Viral Immunol 22:423–430 [View Article][PubMed]
MarkwellM.A.K.1991; New Frontiers Opened by the Exploration of Host Cell Receptors. In The Paramyxoviruses pp. 407–425 Edited by
KingsburyD. W.
Boston, MA: Springer US; [View Article]
MatrosovichM.N.,
MatrosovichT.Y.,
GrayT.,
RobertsN.A.,
KlenkH.-D.2004; Human and avian influenza viruses target different cell types in cultures of human airway epithelium. Proc Natl Acad Sci U S A 101:4620–4624 [View Article][PubMed]
OhishiK.,
NinomiyaA.,
KidaH.,
ParkC.-H.,
MaruyamaT.,
AraiT.,
KatsumataE.,
TobayamaT.,
BoltunovA.N.,
other authors.
2002; Serological evidence of transmission of human influenza A and B viruses to Caspian seals (Phoca caspica). Microbiol Immunol 46:639–644 [View Article][PubMed]
ParkerL.,
GillilandS.M.,
MinorP.,
SchepelmannS.2013; Assessment of the ferret as an in vivo model for mumps virus infection. J Gen Virol 94:1200–1205 [View Article][PubMed]
PilletS.,
SvitekN.,
von MesslingV.2009; Ferrets as a model for morbillivirus pathogenesis, complications, and vaccines. Curr Top Microbiol Immunol 330:73–87[PubMed]
RobertsK.L.,
SheltonH.,
ScullM.,
PicklesR.,
BarclayW.S.2011; Lack of transmission of a human influenza virus with avian receptor specificity between ferrets is not due to decreased virus shedding but rather a lower infectivity in vivo. J Gen Virol 92:1822–1831 [View Article][PubMed]
RobertsK.L.,
SheltonH.,
StilwellP.,
BarclayW.S.2012; Transmission of a 2009 H1N1 pandemic influenza virus occurs before fever is detected, in the ferret model. PLoS One 7:e43303[CrossRef]
ThompsonC.I.,
BarclayW.S.,
ZambonM.C.,
PicklesR.J.2006; Infection of human airway epithelium by human and avian strains of influenza a virus. J Virol 80:8060–8068 [View Article][PubMed]
van RielD.,
MunsterV.J.,
de WitE.,
RimmelzwaanG.F.,
FouchierR.A.M.,
OsterhausA.D.M.E.,
KuikenT.2007; Human and avian influenza viruses target different cells in the lower respiratory tract of humans and other mammals. Am J Pathol 171:1215–1223 [View Article][PubMed]
WangY.F.,
ChangC.F.,
ChiC.Y.,
WangH.C.,
WangJ.R.,
SuI.J.2012; Characterization of glycan binding specificities of influenza B viruses with correlation with hemagglutinin genotypes and clinical features. J Med Virol 84:679–685 [View Article][PubMed]