The family Coronaviridae represents a diverse group of vertebrate RNA viruses, all with genomes greater than 26 000 nt. Here, we report the discovery and genetic characterization of a novel virus present in cattle with respiratory disease. Phylogenetic characterization of this virus revealed that it clusters within the subfamily Torovirinae, in the family Coronaviridae. The complete genome consists of only 20 261 nt and represents the smallest reported coronavirus genome. We identified seven ORFs, including the canonical nidovirus ORF1a and ORF1b. Analysis of polyprotein 1ab revealed that this virus, tentatively named bovine nidovirus (BoNV), shares the highest homology with the recently described python-borne nidoviruses and contains several conserved nidovirus motifs, but does not encode the NendoU or O-MT domains that are present in other viruses within the family Coronaviridae. In concert with its reduced genome, the atypical domain architecture indicates that this virus represents a unique lineage within the order Nidovirales.
BeardsG.M., GreenJ., HallC., FlewettT.H., LamouliatteF., Du PasquierP.1984; An enveloped virus in stools of children and adults with gastroenteritis that resembles the Breda virus of calves. Lancet 323:1050–1052 [View Article][PubMed]
BodewesR., LemppC., SchürchA.C., HabierskiA., HahnK., LamersM., von DörnbergK., WohlseinP., DrexlerJ.F., other authors. 2014; Novel divergent nidovirus in a python with pneumonia. J Gen Virol 95:2480–2485 [View Article][PubMed]
BurbeloP.D., ChingK.H., KlimaviczC.M., IadarolaM.J.2009; Antibody profiling by luciferase immunoprecipitation systems (LIPS). J Vis Exp 32:1549[PubMed]
deGrootR.J., CowleyJ.A., EnjuanesL., FaabergK.S., PerlmanS., RottierP.J.M., SnijderE.J., ZiebuhrJ., GorbalenyaA.E.2011a; Order Nidovirales
. In Virus Taxonomy; Ninth Report of the International Committee on Taxonomy of Viruses pp. 782–795 Edited by KingA. M. Q., AdamsM. J., CarstensE. B., LefkowitzE. J. Oxford: Elsevier Academic Press;
deGrootR.J., BakerS.C., BaricR., EnjuanesL., GorbalenyaA.E., HolmesK.V., PerlmanS., PoonL., RottierP.J.M., other authors. 2011b; Family Coronaviridae
. In Virus Taxonomy; Ninth Report of the International Committee on Taxonomy of Viruses pp. 806–828 Edited by KingA. M. Q., AdamsM. J., CarstensE. B., LefkowitzE. J. Oxford: Elsevier Academic Press;
LauberC., ZiebuhrJ., JunglenS., DrostenC., ZirkelF., NgaP.T., MoritaK., SnijderE.J., GorbalenyaA.E.2012; Mesoniviridae: a proposed new family in the order Nidovirales formed by a single species of mosquito-borne viruses. Arch Virol 157:1623–1628 [View Article][PubMed]
LauberC., GoemanJ.J., ParquetM.C., NgaP.T., SnijderE.J., MoritaK., GorbalenyaA.E.2013; The footprint of genome architecture in the largest genome expansion in RNA viruses. PLoS Pathog 9:e1003500 [View Article][PubMed]
NgaP.T., ParquetM.C., LauberC., ParidaM., NabeshimaT., YuF., ThuyN.T., InoueS., ItoT., other authors. 2011; Discovery of the first insect nidovirus, a missing evolutionary link in the emergence of the largest RNA virus genomes. PLoS Pathog 7:e1002215 [View Article][PubMed]
UccelliniL., OssiboffR.J., de MatosR.E., MorriseyJ.K., PetrosovA., Navarrete-MaciasI., JainK., HicksA.L., BucklesE.L., other authors. 2014; Identification of a novel nidovirus in an outbreak of fatal respiratory disease in ball pythons (Python regius). Virol J 11:144 [View Article][PubMed]
WeissM., SteckF., HorzinekM.C.1983; Purification and partial characterization of a new enveloped RNA virus (Berne virus). J Gen Virol 64:1849–1858 [View Article][PubMed]